Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 322.2 9
0297-0624
218.76 623.81 309 63.60 0.960
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 377.4 9
0298-0628
473.80 627.67 178 52.53 0.811
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 342.8 9
0299-0629
88.26 629.56 283 62.53 0.910
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 330.4 9
0300-0629
777.02 628.82 261 61.01 0.881
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 235.6 9
0301-0635
568.32 635.08 290 73.84 0.668
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 314.7 9
0302-0631
810.05 630.69 249 63.26 0.782
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.1 9
0303-0630
383.60 630.36 218 53.36 0.962
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 311.2 9
0304-0632
640.10 632.53 232 59.70 0.818
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 357.5 9
0305-0633
902.89 632.87 350 68.18 0.946
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.3 9
0306-0631
325.68 632.39 263 60.77 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 408.7 9
0307-0633
688.22 633.46 306 63.94 0.941
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 243.9 9
0308-0640
182.52 640.32 330 69.60 0.856
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 338.1 9
0309-0639
110.84 638.95 229 56.53 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 345.7 9
0310-0642
264.97 641.97 190 53.36 0.839
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 244.5 9
0311-0642
946.31 642.26 211 55.94 0.847
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 539.3 9
0312-0653
717.73 653.94 494 85.15 0.856
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 278.4 9
0313-0650
429.31 649.95 196 52.28 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.8 9
0314-0655
504.15 656.17 268 64.18 0.818
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.6 9
0315-0652
657.70 652.90 261 59.11 0.939
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 347.9 9
0316-0654
70.78 654.67 174 51.11 0.837
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 280.0 9
0317-0657
339.71 657.78 226 56.53 0.889
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.8 9
0318-0655
383.03 655.64 149 48.87 0.784
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.5 9
0319-0657
200.29 656.11 247 59.84 0.867
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 343.5 9
0320-0658
839.59 659.58 154 49.70 0.784
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.8 9
0321-0657
444.85 658.14 127 47.11 0.719