Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 265.9 7
0260-0480
 758.16 480.43 318 69.60 0.825
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 339.4 7
0261-0489
 489.59 489.12 471 81.26 0.896
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 323.6 7
0262-0486
 905.96 486.58 296 64.18 0.903
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 423.7 7
0263-0490
 579.07 489.41 186 57.60 0.705
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 417.8 7
0264-0493
 259.07 493.34 275 60.77 0.936
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.8 7
0265-0497
 773.89 497.11 282 64.77 0.845
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 381.3 7
0266-0497
 234.03 496.95 259 58.53 0.950
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 304.8 7
0267-0502
 658.63 502.20 266 60.18 0.923
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 208.3 7
0268-0503
 846.58 503.79 333 68.18 0.900
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 247.2 7
0269-0506
 122.20 506.82 276 62.53 0.887
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 212.1 7
0270-0508
 532.58 508.24 317 65.60 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 549.8 7
0271-0513
 340.54 511.95 653 107.40 0.711
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.1 7
0272-0511
 201.18 511.35 298 66.18 0.855
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 272.3 7
0273-0509
 374.99 510.00 260 58.77 0.946
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 254.2 7
0274-0514
 779.70 514.47 297 65.36 0.874
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 250.2 7
0275-0514
 163.92 514.24 276 63.60 0.857
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.6 7
0276-0515
 474.02 515.23 306 64.43 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.6 7
0277-0520
 285.23 519.48 281 66.43 0.800
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.7 7
0278-0523
 554.66 522.46 299 65.60 0.873
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 254.7 7
0279-0527
 595.61 527.94 333 69.01 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 285.4 7
0280-0530
 462.19 530.53 273 65.26 0.806
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 200.5 7
0281-0531
 50.04 531.24 281 65.01 0.835
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 211.8 7
0282-0532
 83.20 532.26 287 62.18 0.933
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 393.9 7
0283-0538
 235.20 538.13 469 80.08 0.919
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 247.0 7
0284-0537
 720.99 537.06 278 66.43 0.792