Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 398.5 1
0107-0404
 622.35 404.81 425 77.26 0.895
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.4 1
0108-0408
 927.12 408.71 622 92.91 0.905
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2562.7 1
0109-0408
 202.68 407.87 460 82.08 0.858
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 390.2 1
0110-0410
 447.45 410.02 447 79.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 726.1 1
0111-0410
 524.60 409.87 428 77.26 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2110.2 1
0112-0412
 230.55 412.57 450 80.08 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 931.1 1
0113-0421
 62.27 421.92 824 114.71 0.787
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 269.1 1
0114-0414
 389.83 414.33 420 76.91 0.892
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 890.3 1
0115-0418
 264.20 418.41 416 76.67 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1331.0 1
0116-0419
 884.01 419.03 418 77.26 0.880
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 190.6 1
0117-0420
 149.68 421.22 460 85.50 0.791
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 295.9 1
0118-0424
 786.24 424.27 462 82.18 0.860
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 281.9 1
0119-0424
 585.75 424.81 484 82.08 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 230.3 1
0120-0424
 113.98 424.14 456 78.67 0.926
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3868.8 1
0121-0430
 738.05 430.83 419 75.84 0.915
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 372.6 1
0122-0437
 548.25 437.22 430 77.26 0.905
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1096.8 1
0123-0443
 679.62 443.50 452 78.43 0.923
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1604.2 1
0124-0452
 452.68 451.69 956 125.68 0.761
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 891.9 1
0125-0455
 21.35 455.63 811 114.33 0.780
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 392.5 1
0126-0448
 861.22 449.47 388 74.04 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 410.6 1
0127-0452
 942.36 452.57 419 78.43 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 603.2 1
0128-0455
 97.13 455.50 413 79.84 0.814
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 444.6 1
0129-0458
 378.27 458.43 435 76.67 0.930
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 605.5 1
0130-0461
 192.39 459.84 436 78.67 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2152.3 1
0131-0460
 249.49 459.97 406 74.77 0.913