Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1228.9 9
0074-0223
 739.20 223.72 392 76.08 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1201.2 9
0075-0227
 205.50 226.76 434 80.77 0.836
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 276.5 9
0076-0233
 522.04 232.65 722 103.40 0.849
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 375.7 9
0077-0233
 717.89 233.06 548 93.40 0.789
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1658.7 9
0078-0232
 882.86 231.82 374 71.60 0.917
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 805.8 9
0079-0242
 370.93 242.04 567 91.74 0.847
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 285.6 9
0080-0244
 793.57 244.69 404 75.26 0.896
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1187.1 9
0081-0247
 296.20 247.12 377 73.01 0.889
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 864.0 9
0082-0251
 259.12 251.10 369 72.43 0.884
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 284.4 9
0083-0251
 549.31 251.59 444 78.67 0.902
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 373.1 9
0084-0250
 180.46 250.07 376 73.84 0.867
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1158.8 9
0085-0252
 587.08 251.82 386 75.84 0.843
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 709.3 9
0086-0255
 153.26 255.91 388 75.50 0.855
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1053.8 9
0087-0256
 77.61 256.50 386 74.43 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2925.0 9
0088-0258
 514.86 258.37 395 75.84 0.863
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 389.6 9
0089-0258
 846.26 258.51 396 73.01 0.933
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 318.1 9
0090-0264
 324.38 263.88 658 94.91 0.918
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 413.9 9
0091-0262
 671.84 262.19 432 80.91 0.829
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 672.4 9
0092-0257
 915.92 257.74 364 71.94 0.884
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 502.2 9
0093-0264
 887.34 264.27 349 75.36 0.772
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 10702.0 9
0094-0273
 410.97 273.28 409 75.01 0.913
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 384.4 9
0095-0274
 472.81 274.16 308 64.77 0.923
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1011.5 9
0096-0280
 581.72 280.94 420 76.08 0.912
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5334.0 9
0097-0281
 658.64 281.64 406 74.67 0.915
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 469.1 9
0098-0283
 791.94 282.76 373 73.01 0.879