Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2212.3 5
0022-0063
305.30 62.56 282 66.67 0.797
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 706.8 5
0023-0062
186.19 63.10 290 65.60 0.847
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2822.9 5
0024-0064
806.78 64.54 201 56.43 0.793
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 657.2 5
0025-0067
26.26 66.14 274 64.43 0.830
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 247.9 5
0026-0066
117.58 65.66 227 59.60 0.803
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 912.8 5
0027-0071
231.30 71.55 281 65.60 0.821
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1116.1 5
0028-0071
753.60 71.94 276 61.36 0.921
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 846.1 5
0029-0072
559.09 72.36 265 60.77 0.902
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 219.6 5
0030-0076
379.29 75.97 313 67.01 0.876
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 254.1 5
0031-0080
123.83 80.49 366 76.18 0.792
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 692.1 5
0032-0075
165.25 74.41 288 65.70 0.838
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 176.5 5
0033-0078
613.99 77.76 256 58.53 0.939
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 176.0 5
0034-0079
923.97 79.38 259 59.70 0.913
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 228.4 5
0035-0081
60.39 81.99 265 64.18 0.808
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1496.2 5
0036-0084
505.04 84.26 310 65.94 0.896
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 749.8 5
0037-0090
693.12 90.15 316 68.18 0.854
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1315.5 5
0038-0088
724.22 87.61 299 63.60 0.929
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1779.2 5
0039-0087
254.08 87.36 238 58.77 0.866
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 291.0 5
0040-0089
87.63 89.57 252 58.53 0.924
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 228.8 5
0041-0090
184.69 90.96 419 81.26 0.797
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 335.4 5
0042-0094
281.23 94.20 346 70.18 0.883
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 204.3 5
0043-0093
162.46 93.63 217 59.70 0.765
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 452.5 5
0044-0097
316.17 98.03 272 60.77 0.926
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 776.4 5
0045-0105
858.99 106.45 309 76.77 0.659
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 835.1 5
0046-0101
651.89 101.10 345 69.84 0.889