Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 284.1 4
0349-0701
733.39 701.49 303 67.36 0.839
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3597.4 4
0350-0705
877.22 705.20 108 42.04 0.768
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 165.4 4
0351-0707
930.89 707.77 266 65.01 0.791
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 388.6 4
0352-0710
358.48 709.73 291 63.94 0.894
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 306.0 5
0001-0025
207.22 24.73 281 63.36 0.880
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 203.3 5
0002-0023
628.36 23.18 228 56.28 0.904
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 291.4 5
0003-0027
757.57 26.69 263 60.43 0.905
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 345.0 5
0004-0025
245.80 25.00 263 60.53 0.902
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 416.1 5
0005-0035
174.04 35.25 268 60.77 0.912
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 373.5 5
0006-0036
402.63 35.76 271 61.36 0.905
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 827.2 5
0007-0037
66.40 37.22 253 59.11 0.910
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 758.6 5
0008-0039
746.47 39.01 243 59.84 0.853
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 210.0 5
0009-0041
937.37 41.10 272 64.18 0.830
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 662.6 5
0010-0045
583.63 46.23 377 80.18 0.737
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1466.4 5
0011-0041
602.48 40.47 210 54.87 0.877
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2194.2 5
0012-0046
510.44 45.61 319 65.01 0.948
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 254.5 5
0013-0047
417.06 47.14 262 70.67 0.659
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3079.2 5
0014-0048
325.88 48.14 252 59.70 0.889
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 308.1 5
0015-0048
864.11 48.79 362 71.01 0.902
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1188.7 5
0016-0051
538.31 51.75 284 63.94 0.873
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3468.0 5
0017-0049
663.18 49.26 245 56.77 0.955
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3294.9 5
0018-0051
47.74 51.33 240 58.18 0.891
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3092.6 5
0019-0051
887.77 51.56 252 57.94 0.943
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 395.4 5
0020-0056
264.29 55.56 497 91.74 0.742
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 4646.4 5
0021-0055
482.62 55.53 232 56.77 0.905