Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0212 0.1292 0.0281 0.0120 0.0047 0.0084 0.0027 0.0043 0.0045 0.1389 0.0243 0.0478 0.0094 0.0045 0.0039 0.0040 0.0055 0.0041 0.0228 0.0445
WM1552C 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0273 0.1431 0.0285 0.0128 0.0048 0.0088 0.0027 0.0043 0.0042 0.1451 0.0322 0.0211 0.0614 0.0108 0.0042 0.0035 0.0046 0.0064 0.0042 0.0252 0.0583
WM1552C 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0358 0.2203 0.0409 0.0146 0.0068 0.0128 0.0034 0.0057 0.0069 0.1855 0.0303 0.0194 0.0647 0.0137 0.0067 0.0050 0.0058 0.0071 0.0046 0.0292 0.0921
WM1552C 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0407 0.1996 0.0389 0.0176 0.0063 0.0110 0.0029 0.0051 0.0062 0.1043 0.0307 0.0215 0.0953 0.0143 0.0065 0.0049 0.0056 0.0047 0.0324 0.1121
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0215 0.2439 0.0305 0.0214 0.0054 0.0056 0.0035 0.0076 0.0055 0.0182 0.0262 0.0217 0.0582 0.0179 0.0051 0.0073 0.0067 0.0075 0.0045 0.0429 0.0500
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0266 0.2890 0.0377 0.0276 0.0049 0.0052 0.0030 0.0052 0.0058 0.0203 0.0334 0.0248 0.0890 0.0209 0.0043 0.0095 0.0047 0.0082 0.0046 0.0527 0.0590
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0235 0.2004 0.0232 0.0157 0.0047 0.0037 0.0030 0.0035 0.0040 0.0274 0.0198 0.0163 0.0493 0.0165 0.0047 0.0074 0.0048 0.0051 0.0040 0.0434 0.0584
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0267 0.2710 0.0282 0.0188 0.0046 0.0043 0.0031 0.0044 0.0044 0.0265 0.0299 0.0191 0.0699 0.0178 0.0042 0.0096 0.0040 0.0056 0.0051 0.0505 0.0736
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0446 0.8403 0.1149 0.0667 0.0195 0.0184 0.0118 0.0177 0.0182 0.0378 0.0975 0.0858 0.1838 0.0645 0.0154 0.0200 0.0166 0.0221 0.0130 0.1437 0.1192
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0518 1.1364 0.0961 0.0806 0.0222 0.0174 0.0117 0.0187 0.0174 0.0432 0.1103 0.0890 0.2494 0.0642 0.0153 0.0178 0.0206 0.0238 0.0147 0.1575 0.1471
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0250 0.3831 0.0548 0.0407 0.0083 0.0057 0.0047 0.0062 0.0059 0.0137 0.0402 0.0284 0.0871 0.0291 0.0060 0.0116 0.0065 0.0078 0.0050 0.0593 0.0568
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0322 0.4808 0.0703 0.0510 0.0102 0.0102 0.0056 0.0048 0.0079 0.0143 0.0567 0.0333 0.1276 0.0309 0.0052 0.0108 0.0088 0.0124 0.0049 0.0643 0.0979
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0197 0.2494 0.0484 0.0305 0.0145 0.0051 0.0039 0.0052 0.0050 0.0286 0.0314 0.0223 0.0685 0.0218 0.0050 0.0082 0.0059 0.0058 0.0045 0.0401 0.0439
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0230 0.3185 0.0504 0.0372 0.0175 0.0064 0.0042 0.0036 0.0058 0.0304 0.0394 0.0237 0.0915 0.0219 0.0056 0.0081 0.0069 0.0070 0.0052 0.0448 0.0689
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0240 0.1758 0.0468 0.0132 0.0284 0.0037 0.0027 0.0045 0.0043 0.0205 0.0812 0.0542 0.0212 0.0047 0.0078 0.0050 0.0043 0.0042 0.0456 0.0677
WM1552C 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0318 0.2976 0.0578 0.0150 0.0438 0.0061 0.0032 0.0055 0.0068 0.0698 0.0298 0.0840 0.0881 0.0248 0.0073 0.0098 0.0051 0.0082 0.0056 0.0531 0.1103
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0361 0.6329 0.0796 0.0526 0.0139 0.0130 0.0079 0.0111 0.0124 0.0336 0.0767 0.0555 0.1383 0.0422 0.0118 0.0157 0.0172 0.0221 0.0099 0.1060 0.0968
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0491 0.7752 0.0812 0.0676 0.0139 0.0145 0.0085 0.0111 0.0117 0.0366 0.0840 0.0569 0.1890 0.0483 0.0123 0.0134 0.0139 0.0211 0.0109 0.1114 0.1379
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0219 0.3226 0.0433 0.0324 0.0065 0.0055 0.0041 0.0051 0.0052 0.0209 0.0353 0.0246 0.0650 0.0231 0.0056 0.0094 0.0062 0.0064 0.0048 0.0500 0.0533
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0308 0.4115 0.0549 0.0448 0.0087 0.0106 0.0046 0.0043 0.0064 0.0222 0.0473 0.0281 0.1017 0.0237 0.0049 0.0092 0.0079 0.0117 0.0060 0.0576 0.0862
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0312 0.5917 0.0944 0.0501 0.0457 0.0097 0.0074 0.0110 0.0106 0.0287 0.0611 0.0459 0.1250 0.0414 0.0092 0.0162 0.0117 0.0155 0.0087 0.0893 0.0814