Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0251 0.3891 0.0544 0.0247 0.0104 0.0118 0.0046 0.0064 0.0063 0.0200 0.0468 0.0269 0.0970 0.0159 0.0065 0.0061 0.0081 0.0078 0.0059 0.0372 0.0611
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0330 0.5051 0.0648 0.0332 0.0143 0.0181 0.0062 0.0058 0.0087 0.0210 0.0668 0.0321 0.1468 0.0166 0.0058 0.0055 0.0089 0.0118 0.0074 0.0409 0.1111
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0368 0.5682 0.0825 0.0397 0.0160 0.0272 0.0091 0.0130 0.0132 0.0354 0.1306 0.0579 0.1707 0.0338 0.0144 0.0109 0.0125 0.0168 0.0142 0.0712 0.0943
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0447 0.7692 0.0952 0.0505 0.0194 0.0331 0.0089 0.0084 0.0151 0.0410 0.1623 0.0638 0.2404 0.0302 0.0155 0.0082 0.0144 0.0232 0.0118 0.0786 0.1543
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0180 0.1835 0.0322 0.0159 0.0057 0.0100 0.0030 0.0042 0.0040 0.0179 0.0397 0.0175 0.0593 0.0092 0.0045 0.0041 0.0042 0.0047 0.0036 0.0216 0.0418
WM1552C 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0264 0.2488 0.0481 0.0232 0.0091 0.0164 0.0031 0.0055 0.0067 0.0184 0.0598 0.0220 0.0917 0.0120 0.0067 0.0047 0.0058 0.0110 0.0043 0.0245 0.0762
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0363 0.6944 0.0894 0.0644 0.0269 0.0227 0.0088 0.0153 0.0159 0.0409 0.0939 0.0575 0.1587 0.0309 0.0168 0.0125 0.0127 0.0239 0.0122 0.0701 0.1079
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0482 0.8000 0.0906 0.0538 0.0266 0.0289 0.0095 0.0130 0.0155 0.0429 0.1018 0.0585 0.2155 0.0405 0.0137 0.0084 0.0134 0.0265 0.0129 0.0809 0.1597
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0277 0.4975 0.0691 0.0350 0.0119 0.0162 0.0061 0.0087 0.0078 0.0260 0.0616 0.0361 0.1019 0.0223 0.0081 0.0082 0.0088 0.0124 0.0073 0.0348 0.0772
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0406 0.6135 0.0906 0.0494 0.0161 0.0263 0.0083 0.0063 0.0110 0.0272 0.0859 0.0442 0.1773 0.0231 0.0082 0.0074 0.0130 0.0230 0.0102 0.0427 0.1328
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0339 0.5181 0.0872 0.0420 0.0168 0.0247 0.0083 0.0146 0.0135 0.0394 0.1245 0.0546 0.1405 0.0341 0.0124 0.0122 0.0114 0.0214 0.0112 0.0535 0.0903
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0499 0.6329 0.0984 0.0658 0.0195 0.0377 0.0100 0.0141 0.0165 0.0391 0.1437 0.0602 0.2101 0.0343 0.0158 0.0133 0.0153 0.0316 0.0131 0.0623 0.1567
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0209 0.1923 0.0321 0.0171 0.0078 0.0114 0.0032 0.0043 0.0048 0.0256 0.0433 0.0199 0.0645 0.0122 0.0052 0.0046 0.0046 0.0058 0.0047 0.0230 0.0521
WM1552C 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0299 0.2732 0.0514 0.0252 0.0115 0.0188 0.0047 0.0062 0.0077 0.0244 0.0631 0.0261 0.1028 0.0137 0.0064 0.0065 0.0071 0.0132 0.0051 0.0289 0.0824
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0321 0.4831 0.0869 0.0394 0.0262 0.0250 0.0088 0.0145 0.0136 0.0450 0.0839 0.0501 0.1437 0.0320 0.0109 0.0091 0.0117 0.0193 0.0099 0.0461 0.0865
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0463 0.6369 0.0872 0.0463 0.0242 0.0270 0.0081 0.0117 0.0133 0.0506 0.0993 0.0456 0.1953 0.0344 0.0114 0.0077 0.0132 0.0264 0.0112 0.0580 0.1174
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0275 0.3497 0.0644 0.0295 0.0129 0.0168 0.0057 0.0084 0.0098 0.0404 0.0582 0.0318 0.0921 0.0202 0.0084 0.0062 0.0081 0.0150 0.0068 0.0376 0.0669
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0339 0.4695 0.0776 0.0368 0.0152 0.0202 0.0059 0.0076 0.0091 0.0397 0.0720 0.0316 0.1473 0.0226 0.0072 0.0047 0.0087 0.0170 0.0065 0.0417 0.0976
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0420 0.6098 0.1065 0.0514 0.0361 0.0368 0.0117 0.0186 0.0197 0.0428 0.1431 0.0769 0.1761 0.0438 0.0173 0.0145 0.0177 0.0364 0.0154 0.0749 0.1114
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0577 0.7519 0.1306 0.0682 0.0316 0.0421 0.0117 0.0115 0.0188 0.0444 0.1848 0.0769 0.2618 0.0499 0.0165 0.0090 0.0177 0.0426 0.0156 0.0838 0.1647
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0237 0.1695 0.0376 0.0183 0.0063 0.0105 0.0032 0.0050 0.0066 0.0452 0.0376 0.0201 0.0543 0.0122 0.0050 0.0039 0.0046 0.0077 0.0043 0.0192 0.0432
WM1552C 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0260 0.1848 0.0458 0.0190 0.0059 0.0124 0.0026 0.0046 0.0056 0.0484 0.0234 0.0831 0.0119 0.0046 0.0040 0.0047 0.0091 0.0038 0.0220 0.0595
WM1552C 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0206 0.2907 0.0629 0.0267 0.0151 0.0134 0.0061 0.0084 0.0083 0.0343 0.0493 0.0297 0.0880 0.0244 0.0071 0.0057 0.0076 0.0162 0.0066 0.0238 0.0588
WM1552C 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0254 0.3610 0.0577 0.0298 0.0181 0.0160 0.0051 0.0077 0.0070 0.0341 0.0648 0.0318 0.1348 0.0205 0.0053 0.0052 0.0080 0.0143 0.0071 0.0266 0.0696
WM1552C 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0207 0.2336 0.0415 0.0180 0.0091 0.0108 0.0047 0.0054 0.0052 0.0278 0.0364 0.0205 0.0712 0.0135 0.0058 0.0047 0.0062 0.0091 0.0047 0.0216 0.0560