Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0549 0.4808 0.0043 0.0036 0.0063 0.0107 0.0108 0.0173 0.0615 0.3086 0.0363 0.0228 0.0076 0.0252 0.0067 0.0234 0.0103 0.0040 0.0650 0.0169
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0574 0.4292 0.0032 0.0096 0.0099 0.0100 0.0176 0.0843 0.3311 0.0731 0.0245 0.0091 0.0241 0.0084 0.0263 0.0145 0.0059 0.0506 0.0273
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0608 0.4292 0.0048 0.0037 0.0086 0.0100 0.0104 0.0158 0.0706 0.3215 0.0722 0.0039 0.0239 0.0083 0.0234 0.0073 0.0290 0.0092 0.0064 0.0837 0.0169
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0571 0.4367 0.0041 0.0031 0.0068 0.0085 0.0132 0.0150 0.0577 0.0452 0.0032 0.0238 0.0073 0.0255 0.0074 0.0205 0.0134 0.0051 0.0464 0.0163
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.4184 0.0043 0.0034 0.0071 0.0089 0.0101 0.0153 0.0632 0.3096 0.0490 0.0036 0.0239 0.0070 0.0244 0.0069 0.0227 0.0114 0.0053 0.0517 0.0184
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0583 0.4425 0.0053 0.0035 0.0116 0.0111 0.0167 0.0818 0.3185 0.0800 0.0034 0.0238 0.0078 0.0237 0.0062 0.0320 0.0119 0.0044 0.0429 0.0218
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0583 0.4184 0.0049 0.0037 0.0119 0.0101 0.0120 0.0156 0.0689 0.3279 0.0743 0.0037 0.0244 0.0072 0.0228 0.0059 0.0230 0.0079 0.0051 0.0562 0.0174
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0610 0.0048 0.0028 0.0074 0.0096 0.0107 0.0146 0.0654 0.0505 0.0032 0.0073 0.0369 0.0132 0.0702 0.0135
COLO 858 0.0000
uM
 1
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0423 0.5025 0.0048 0.0045 0.0094 0.0102 0.0077 0.0238 0.0792 0.3802 0.0524 0.0036 0.0223 0.0093 0.0206 0.0083 0.0344 0.0121 0.0047 0.0521 0.0249
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0512 0.7092 0.0045 0.0049 0.0110 0.0108 0.0087 0.0206 0.0899 0.4505 0.0582 0.0036 0.0237 0.0084 0.0304 0.0099 0.0348 0.0129 0.0051 0.0698 0.0311
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0514 0.6098 0.0046 0.0034 0.0088 0.0130 0.0092 0.0201 0.0564 0.5587 0.0328 0.0032 0.0241 0.0086 0.0294 0.0062 0.0534 0.0118 0.0065 0.0751 0.0124
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0541 0.7042 0.0045 0.0040 0.0104 0.0146 0.0101 0.0222 0.0584 0.5263 0.0381 0.0036 0.0253 0.0095 0.0370 0.0054 0.0519 0.0144 0.0056 0.0831 0.0158
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0316 0.5952 0.0037 0.0035 0.0067 0.0078 0.0061 0.0117 0.0611 0.4951 0.0458 0.0025 0.0245 0.0097 0.0281 0.0056 0.0300 0.0080 0.0059 0.0611 0.0167
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0330 0.8547 0.0039 0.0034 0.0066 0.0094 0.0062 0.0131 0.0625 0.4566 0.0482 0.0031 0.0288 0.0097 0.0411 0.0059 0.0325 0.0090 0.0056 0.0580 0.0229
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0479 0.9434 0.0036 0.0030 0.0067 0.0117 0.0078 0.0180 0.0627 0.5236 0.0427 0.0025 0.0081 0.0506 0.0118 0.0061 0.0608 0.0104
COLO 858 0.0000
uM
 5
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0278 0.3390 0.0042 0.0030 0.0074 0.0105 0.0059 0.0119 0.0625 0.6173 0.0438 0.0027 0.0149 0.0208 0.0078 0.0647 0.0099 0.0089 0.0937 0.0119
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0302 0.3953 0.0041 0.0028 0.0081 0.0096 0.0067 0.0118 0.0620 0.5556 0.0392 0.0033 0.0156 0.0070 0.0262 0.0043 0.0709 0.0097 0.0096 0.0142
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0511 0.5917 0.0040 0.0033 0.0070 0.0093 0.0080 0.0155 0.0725 0.3861 0.0381 0.0029 0.0242 0.0086 0.0334 0.0054 0.0428 0.0072 0.0053 0.0653 0.0232
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0517 0.6098 0.0046 0.0039 0.0068 0.0107 0.0093 0.0184 0.0708 0.3623 0.0421 0.0032 0.0251 0.0095 0.0414 0.0066 0.0433 0.0084 0.0046 0.0693 0.0300
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0341 0.3623 0.0040 0.0031 0.0066 0.0081 0.0074 0.0152 0.0546 0.4115 0.0515 0.0029 0.0165 0.0073 0.0167 0.0057 0.0400 0.0074 0.0057 0.0516 0.0363
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0355 0.4132 0.0036 0.0035 0.0082 0.0103 0.0081 0.0153 0.0614 0.4132 0.0455 0.0040 0.0187 0.0073 0.0187 0.0061 0.0366 0.0092 0.0054 0.0695 0.0436
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0453 0.5747 0.0041 0.0031 0.0078 0.0117 0.0082 0.0149 0.0580 0.4167 0.0372 0.0025 0.0225 0.0081 0.0274 0.0052 0.0366 0.0102 0.0053 0.0593 0.0113
COLO 858 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0490 0.5917 0.0039 0.0077 0.0133 0.0101 0.0191 0.0605 0.4367 0.0400 0.0034 0.0246 0.0093 0.0347 0.0072 0.0382 0.0103 0.0058 0.0668 0.0129