Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.9091 1.8484 0.3937 0.2141 0.2247 0.4717 0.5348 0.5076 1.0000 0.6579 0.7874 0.6250 0.4505 0.5780 0.1815 0.5405 0.3546 0.2604 0.7463 0.8621
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.9615 1.7361 0.3610 0.2041 0.4630 0.6061 0.4739 0.9434 0.6897 0.7752 0.6250 0.4149 0.5988 0.1908 0.5525 0.3559 0.2639 0.8000 0.8929
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.7353 1.4472 0.3559 0.2165 0.1969 0.4167 0.4854 0.4049 0.8696 0.6452 0.6579 0.4878 0.3759 0.5263 0.1739 0.5714 0.3546 0.2353 0.6849 0.8696
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.7576 1.6207 0.3610 0.2160 0.2212 0.4762 0.5618 0.4167 0.9259 0.6410 0.6494 0.4808 0.3802 0.5405 0.1980 0.5714 0.2519 0.7042 0.7246
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.7813 1.6863 0.4484 0.2174 0.2198 0.5650 0.5618 0.5102 1.0718 0.8772 0.8475 0.6289 0.4831 0.6289 0.1754 0.6849 0.3345 0.2857 0.6711 0.6494
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.8065 1.6181 0.4695 0.2096 0.2262 0.5208 0.5587 0.4717 1.0341 0.9524 0.9615 0.6135 0.4329 0.6452 0.1961 0.7692 0.3546 0.3003 0.7299 0.5747
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5952 1.4104 0.3300 0.2045 0.2058 0.3922 0.4951 0.3597 0.9259 0.6135 0.6289 0.4405 0.3300 0.4630 0.2092 0.7194 0.3378 0.2445 0.5650 0.4739
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.6250 1.4706 0.3425 0.2283 0.2188 0.4167 0.5155 0.3802 0.9524 0.6329 0.6024 0.4348 0.3390 0.4546 0.1961 0.7299 0.3597 0.2924 0.5714 0.4292
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.9709 1.9194 0.4546 0.2169 0.2347 0.6369 0.5747 0.5435 1.1148 1.0449 1.0627 0.7143 0.4831 0.6098 0.1873 0.7042 0.3333 0.2916 0.7813 0.7519
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.9524 1.8083 0.4785 0.1961 0.2278 0.5917 0.5376 1.0881 1.0741 1.0560 0.6250 0.4444 0.6849 0.1859 0.7194 0.3718 0.3145 0.8130 0.7092
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.6211 1.8939 0.3279 0.2008 0.1949 0.4184 0.5263 0.3534 0.8772 0.6993 0.5882 0.4184 0.3215 0.4274 0.2045 0.8000 0.3300 0.2597 0.5291 0.4902
MMAC-SF 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.6173 1.9841 0.3165 0.2033 0.2105 0.4739 0.5650 0.3759 0.9346 0.7092 0.6211 0.4098 0.3300 0.4149 0.1996 0.7463 0.3185 0.2564 0.5435 0.4219
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.9174 2.4570 0.5025 0.2874 0.5988 0.6993 0.5128 1.1933 0.8929 0.9709 0.6993 0.4831 0.7042 0.2033 0.6849 0.3759 0.3891 0.7692 0.7463
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.9174 2.1186 0.5208 0.2123 0.2584 0.5376 0.6410 0.4202 1.0309 0.9434 1.0081 0.6536 0.4651 0.7299 0.1984 0.7874 0.4149 0.3584 0.7813 0.7299
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5435 1.3038 0.3401 0.2088 0.2110 0.3125 0.5128 0.3165 0.7874 0.4695 0.5405 0.4098 0.2747 0.4255 0.1869 0.7143 0.3472 0.2500 0.5000 0.5556
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5682 1.3106 0.3597 0.2252 0.2141 0.3135 0.5181 0.2632 0.8621 0.4878 0.5181 0.4237 0.3012 0.4049 0.2075 0.6944 0.3636 0.2695 0.5102 0.4505
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.8475 2.1552 0.4902 0.2070 0.2416 0.5525 0.6494 0.5495 1.0070 0.9346 1.0256 0.6757 0.4785 0.6410 0.1818 0.7407 0.3831 0.3472 0.8065 0.7813
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.9259 2.0408 0.5263 0.2083 0.2513 0.5405 0.6757 0.4329 1.0152 0.9346 1.0060 0.6329 0.4367 0.6135 0.1908 0.7246 0.3953 0.3311 0.8772 0.7246
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7634 1.6313 0.4032 0.1927 0.2033 0.5236 0.5102 0.4132 0.8265 0.8929 0.8621 0.5208 0.3774 0.5917 0.1835 0.7246 0.3484 0.2857 0.6944 0.6289
MMAC-SF 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.9091 1.6920 0.4546 0.2062 0.2494 0.6061 0.6173 0.4202 0.9174 0.9901 0.8197 0.6494 0.4525 0.5848 0.1961 0.6711 0.3436 0.3236 0.7576 0.6849
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 1.0582 3.3333 0.5682 0.2188 0.3279 0.6410 0.8065 0.6329 1.4347 1.0707 1.1455 0.7692 0.5435 0.7634 0.1919 0.6711 0.4505 0.4608 0.8000 0.8475
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 1.0560 3.1153 0.5988 0.1980 0.3390 0.6757 0.8475 0.5348 1.3004 1.0504 1.1494 0.7752 0.5376 0.7937 0.2132 0.6803 0.4310 0.4651 0.8850 0.9901
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.6211 1.6026 0.3484 0.2066 0.3831 0.5128 0.3390 0.8850 0.6173 0.5917 0.4546 0.3106 0.4425 0.1802 0.6329 0.3401 0.2833 0.5263 0.5556
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.6579 1.7094 0.3584 0.2033 0.2232 0.4115 0.5465 0.3623 0.8197 0.6410 0.5882 0.4854 0.3322 0.4348 0.1862 0.6803 0.3390 0.2809 0.5780 0.5405
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 1.1249 3.7594 0.6250 0.2299 0.3731 0.7194 0.9174 0.6757 1.5699 1.0870 1.3624 0.7813 0.5747 0.7576 0.2114 0.6494 0.4525 0.5587 0.9524 0.9346