Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0951 0.9615 0.0066 0.0116 0.0173 0.1166 0.0358 0.5236 0.0576 0.0517 0.0392 0.0176 0.0313 0.1626 0.0328 0.0102 0.1062
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1049 1.0526 0.0058 0.0083 0.0197 0.1383 0.0996 0.0387 0.6536 0.0553 0.0445 0.0425 0.0401 0.0050 0.1845 0.0342 0.0082 0.1297 0.2075
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0722 0.6993 0.0043 0.0065 0.0149 0.1100 0.0668 0.0255 0.4202 0.0353 0.0446 0.0294 0.0148 0.0219 0.0054 0.1135 0.0292 0.0080 0.0975 0.1715
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0721 0.7576 0.0050 0.0104 0.0189 0.0840 0.0854 0.0256 0.5848 0.0371 0.0314 0.0390 0.0139 0.0225 0.0054 0.1292 0.0344 0.0094 0.1454 0.1905
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0728 0.7752 0.0043 0.0076 0.0134 0.0870 0.0743 0.0294 0.4329 0.0449 0.0347 0.0370 0.0127 0.0262 0.0066 0.1182 0.0251 0.0072 0.0872 0.1946
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0732 0.8333 0.0051 0.0073 0.0136 0.0924 0.0884 0.0284 0.5618 0.0491 0.0322 0.0296 0.0154 0.0275 0.0045 0.1218 0.0308 0.0061 0.1033 0.1855
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0679 0.9174 0.0052 0.0054 0.0126 0.0850 0.0562 0.0186 0.4651 0.0395 0.0427 0.0307 0.0150 0.0205 0.0048 0.1136 0.0223 0.0065 0.0923 0.1497
MMAC-SF 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0782 0.7874 0.0066 0.0120 0.0140 0.0720 0.0881 0.0188 0.6579 0.0518 0.0277 0.0396 0.0153 0.0244 0.0059 0.1004 0.0274 0.0082 0.1052 0.1832
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0687 0.5650 0.0092 0.0088 0.0157 0.1341 0.1115 0.0325 0.4082 0.0458 0.0283 0.0290 0.0133 0.0074 0.1333 0.0252 0.0071 0.2375
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0823 0.8197 0.0069 0.0090 0.0209 0.0978 0.1041 0.0331 0.4717 0.0419 0.0386 0.0487 0.0130 0.0291 0.0074 0.1484 0.0230 0.0081 0.1127
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0977 0.8850 0.0115 0.0095 0.0171 0.1754 0.1421 0.0406 0.6494 0.0620 0.0426 0.0332 0.0196 0.0331 0.0103 0.1869 0.0362 0.0088 0.1252 0.2398
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 1.0753 0.0122 0.0125 0.0221 0.1318 0.1385 0.0364 0.8000 0.0621 0.0478 0.0160 0.0102 0.1905 0.0360 0.0095 0.2217
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0942 1.0309 0.0061 0.0081 0.0168 0.0853 0.1233 0.0278 0.5435 0.0431 0.0371 0.0411 0.0144 0.0295 0.0056 0.1639 0.0238 0.0105 0.1106 0.2488
MMAC-SF 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0931 1.0101 0.0071 0.0139 0.0194 0.0838 0.1311 0.0182 0.7353 0.0445 0.0339 0.0473 0.0128 0.0317 0.0064 0.1852 0.0267 0.0089 0.1193 0.2778
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0801 0.7752 0.0091 0.0085 0.0175 0.1035 0.1337 0.0349 0.5076 0.0477 0.0456 0.0327 0.0164 0.0251 0.0082 0.1642 0.0268 0.0090 0.0987 0.2222
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0921 1.0204 0.0050 0.0089 0.0184 0.1148 0.1083 0.0329 0.5348 0.0463 0.0313 0.0399 0.0141 0.0335 0.0053 0.1597 0.0201 0.1239 0.1681
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0725 0.6329 0.0047 0.0062 0.0158 0.0718 0.0877 0.4184 0.0334 0.1215 0.0364 0.0141 0.0221 0.0059 0.1361 0.0213 0.0069 0.0919 0.2137
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0767 0.6667 0.0048 0.0099 0.0278 0.0879 0.1013 0.6897 0.0473 0.1022 0.0386 0.0118 0.0246 0.1468 0.0223 0.0076 0.1114 0.1934
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0723 0.6993 0.0055 0.0065 0.0129 0.0796 0.0970 0.0282 0.3788 0.0423 0.0401 0.0287 0.0153 0.0253 0.0066 0.1341 0.0214 0.0093 0.0836 0.1789
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0808 0.8265 0.0048 0.0093 0.0215 0.0932 0.0844 0.0319 0.5682 0.0473 0.0321 0.0405 0.0119 0.0303 0.0057 0.1351 0.0232 0.0088 0.1095 0.1637
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0947 0.9524 0.0062 0.0083 0.0150 0.0951 0.0792 0.0321 0.5988 0.0410 0.0486 0.0386 0.0158 0.0240 0.0058 0.1553 0.0200 0.0077 0.1311 0.4808
MMAC-SF 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0927 0.9615 0.0070 0.0223 0.0996 0.0303 0.8929 0.0417 0.0420 0.0430 0.0160 0.0262 0.0072 0.1736 0.0276 0.0084 0.1518
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0756 0.6849 0.0054 0.0086 0.0149 0.0886 0.1042 0.0335 0.3831 0.0435 0.0433 0.0313 0.0149 0.0256 0.0071 0.1350 0.0234 0.0084 0.0894 0.2101