Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0808 0.6849 0.0053 0.0062 0.0145 0.0869 0.0784 0.0311 0.4098 0.0390 0.0436 0.0343 0.0142 0.0331 0.0072 0.1282 0.0232 0.0090 0.0716 0.1852
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0850 0.7752 0.0055 0.0070 0.0152 0.0969 0.0793 0.0299 0.5682 0.0414 0.0382 0.0411 0.0166 0.0383 0.0050 0.1433 0.0260 0.0075 0.0828 0.1733
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0838 0.8475 0.0051 0.0060 0.0162 0.0532 0.0533 0.0280 0.4739 0.0388 0.0413 0.0426 0.0213 0.0262 0.0059 0.1212 0.0202 0.0068 0.0856 0.1873
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0878 0.9009 0.0074 0.0201 0.0646 0.0876 0.0246 0.6849 0.0452 0.0278 0.0446 0.0142 0.0254 0.0059 0.1409 0.0266 0.0097 0.0912
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0539 0.5848 0.0040 0.0070 0.0159 0.0525 0.0722 0.0171 0.5155 0.0285 0.0277 0.0350 0.0145 0.0051 0.0940 0.0174 0.0068 0.0623 0.1815
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0631 0.9009 0.0050 0.0052 0.0142 0.0598 0.0521 0.0174 0.4854 0.0320 0.0298 0.0402 0.0112 0.0289 0.0039 0.1018 0.0192 0.0071 0.0823 0.1202
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0784 0.7692 0.0042 0.0059 0.0188 0.0563 0.0676 0.0196 0.4831 0.0317 0.0335 0.0369 0.0118 0.0248 0.0058 0.1418 0.0211 0.0070 0.0832 0.3745
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0805 0.7813 0.0047 0.0072 0.0193 0.0549 0.0778 0.0188 0.6711 0.0379 0.0301 0.0375 0.0121 0.0224 0.0048 0.1618 0.0254 0.0073 0.0960 0.2801
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0602 0.7634 0.0043 0.0059 0.0135 0.0655 0.0595 0.0216 0.4546 0.0363 0.0337 0.0304 0.0142 0.0235 0.0053 0.0932 0.0226 0.0062 0.0667 0.2004
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0649 0.8929 0.0042 0.0064 0.0139 0.0577 0.0640 0.0202 0.5291 0.0392 0.0307 0.0411 0.0140 0.0247 0.0047 0.1049 0.0208 0.0066 0.0737 0.1502
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0752 0.8621 0.0047 0.0051 0.0160 0.0662 0.0607 0.0202 0.5236 0.0390 0.0363 0.0350 0.0148 0.0265 0.0044 0.1242 0.0172 0.0075 0.0906 0.3597
MMAC-SF 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0743 0.9009 0.0051 0.0076 0.0170 0.0643 0.0831 0.0201 0.7246 0.0431 0.0281 0.0407 0.0135 0.0239 0.0050 0.1307 0.0214 0.0082 0.0935 0.3597
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0672 0.5465 0.0052 0.0070 0.0158 0.1518 0.0927 0.0172 0.2747 0.0447 0.0251 0.0361 0.0134 0.0177 0.0054 0.1036 0.0243 0.0103 0.0750 0.1669
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0870 0.5917 0.0060 0.0075 0.0242 0.0791 0.0920 0.0196 0.3460 0.0402 0.0389 0.0508 0.0181 0.0210 0.0057 0.1112 0.0294 0.0089 0.1009 0.1185
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0902 0.5848 0.0050 0.0066 0.0175 0.0694 0.1014 0.0178 0.2681 0.0420 0.0296 0.0365 0.0150 0.0173 0.0052 0.0987 0.0267 0.0116 0.1111 0.1585
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0909 0.7752 0.0056 0.0094 0.0217 0.0787 0.0954 0.0200 0.3968 0.0436 0.0299 0.0422 0.0139 0.0174 0.0060 0.1104 0.0319 0.0100 0.1248 0.1560
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
MMAC-SF 0.0000
uM
 24
hr
 1
InnoScan/Mapix
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0777 0.5319 0.0055 0.0060 0.0149 0.0882 0.1126 0.0296 0.2410 0.0399 0.0386 0.0275 0.0135 0.0220 0.0064 0.1391 0.0208 0.0086 0.0736 0.1499
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0842 0.6369 0.0045 0.0071 0.0171 0.0917 0.0835 0.0286 0.3290 0.0431 0.0285 0.0346 0.0120 0.0255 0.0053 0.1304 0.0202 0.0094 0.0909 0.1410
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0868 0.7874 0.0050 0.0054 0.0180 0.0820 0.0836 0.0218 0.3040 0.0367 0.0369 0.0477 0.0137 0.0196 0.0040 0.1161 0.0210 0.0076 0.0912 0.1733
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0861 0.8929 0.0052 0.0093 0.0244 0.0912 0.1172 0.0202 0.4000 0.0384 0.0303 0.0447 0.0206 0.0050 0.1353 0.0276 0.0084 0.1076 0.1678
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0800 0.5848 0.0055 0.0064 0.0158 0.0861 0.1089 0.0287 0.2278 0.0407 0.0420 0.0273 0.0131 0.0268 0.0072 0.1151 0.0228 0.0069 0.0862 0.1418