Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0870 1.0101 0.0053 0.0072 0.0213 0.1361 0.1044 0.0365 0.5155 0.0519 0.0355 0.0413 0.0185 0.0402 0.0052 0.1621 0.0230 0.0083 0.0658 0.1684
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0656 1.1364 0.0107 0.0092 0.0196 0.0677 0.0660 0.0220 0.3731 0.0324 0.0595 0.0139 0.0464 0.0045 0.1208 0.0253 0.0170 0.0414 0.1479
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1109 1.3333 0.0148 0.0326 0.0362 0.5435 0.0696 0.1174 0.0618 0.0184 0.0447 0.0096 0.0208 0.0193 0.1667
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0717 0.7246 0.0061 0.0065 0.0165 0.0836 0.0993 0.0298 0.3030 0.0450 0.0483 0.0308 0.0183 0.0264 0.0076 0.1285 0.0239 0.0087 0.0560 0.1812
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0792 0.9009 0.0056 0.0086 0.0222 0.1253 0.0861 0.0308 0.4951 0.0483 0.0322 0.0394 0.0131 0.0308 0.0053 0.1471 0.0229 0.0105 0.0742 0.1634
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0907 1.0000 0.0159 0.0067 0.0207 0.0517 0.0465 0.0182 0.4785 0.0409 0.0598 0.0418 0.0174 0.0176 0.0048 0.0851 0.0239 0.0123 0.0564
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0875 0.9901 0.0092 0.0129 0.0294 0.0773 0.0716 0.0151 0.7092 0.0418 0.0423 0.0449 0.0149 0.0172 0.0070 0.0966 0.0294 0.0102 0.0652 0.1721
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0784 0.7874 0.0060 0.0123 0.0197 0.0976 0.1518 0.0350 0.3584 0.0588 0.0516 0.0365 0.0175 0.0343 0.0096 0.1464 0.0425 0.0109 0.0522 0.2703
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0954 0.9901 0.0063 0.0067 0.0210 0.1186 0.0867 0.0404 0.5236 0.0619 0.0533 0.0400 0.0434 0.0057 0.1695 0.0381 0.0578 0.2632
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0595 0.5525 0.0043 0.0044 0.0144 0.0539 0.0479 0.0200 0.3058 0.0373 0.0452 0.0305 0.0116 0.0198 0.0053 0.0910 0.0224 0.0056 0.0390 0.1335
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0608 0.6289 0.0040 0.0073 0.0169 0.0564 0.0639 0.0172 0.4566 0.0421 0.0288 0.0289 0.0144 0.0190 0.0049 0.0951 0.0221 0.0075 0.0464 0.1410
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0797 0.7519 0.0053 0.0066 0.0197 0.0903 0.0840 0.0327 0.3356 0.0489 0.0489 0.0339 0.0147 0.0304 0.0088 0.1292 0.0314 0.0101 0.0570 0.2119
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0831 0.8696 0.0060 0.0085 0.0184 0.1035 0.0883 0.0339 0.4630 0.0461 0.0430 0.0364 0.0176 0.0334 0.0056 0.1486 0.0327 0.0069 0.0696 0.2101
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0979 0.9091 0.0054 0.0074 0.0223 0.0842 0.0874 0.0251 0.5465 0.0328 0.0587 0.0432 0.0136 0.0307 0.0065 0.1664 0.0264 0.0087 0.0617 0.3115
MMAC-SF 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0828 0.9615 0.0134 0.0268 0.0953 0.1292 0.0228 0.6452 0.0373 0.0306 0.0438 0.0155 0.0298 0.0067 0.1795 0.0360 0.0094 0.0715 0.3968
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0695 0.7042 0.0054 0.0071 0.0183 0.0811 0.0298 0.4762 0.0416 0.0323 0.0329 0.0144 0.0217 0.0071 0.1172 0.0251 0.0075 0.0470 0.2639
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0763 0.8197 0.0063 0.0054 0.0182 0.0930 0.0672 0.0277 0.4484 0.0446 0.0410 0.0155 0.0045 0.1376 0.0288 0.0072 0.0538 0.1718
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0581 0.5650 0.0034 0.0043 0.0152 0.0513 0.0518 0.0152 0.3049 0.0276 0.0358 0.0294 0.0115 0.0202 0.0048 0.1018 0.0190 0.0061 0.0446 0.2857
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0615 0.5714 0.0042 0.0051 0.0163 0.0482 0.0535 0.0156 0.5155 0.0317 0.0318 0.0276 0.0107 0.0174 0.0041 0.0884 0.0236 0.0065 0.0529 0.1475
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0571 0.5650 0.0040 0.0070 0.0200 0.0670 0.0614 0.0236 0.3300 0.0332 0.0341 0.0322 0.0115 0.0187 0.0055 0.0945 0.0211 0.0057 0.0486 0.2336
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0610 0.5917 0.0042 0.0056 0.0154 0.0663 0.0661 0.0208 0.3817 0.0393 0.0333 0.0338 0.0120 0.0196 0.0037 0.1075 0.0220 0.0059 0.0532 0.1751
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 5
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0633 0.6452 0.0051 0.0081 0.0137 0.0922 0.0240 0.4310 0.0314 0.0251 0.0295 0.0135 0.0232 0.0062 0.1250 0.0237 0.0781 0.2591
MMAC-SF 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0713 0.6993 0.0059 0.0073 0.0161 0.0824 0.0841 0.0221 0.4608 0.0326 0.0437 0.0422 0.0117 0.0266 0.0051 0.1341 0.0198 0.0096 0.1072 0.1520