Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0569 0.5128 0.0047 0.0041 0.0107 0.0656 0.0478 0.0228 0.2976 0.0226 0.0454 0.0226 0.0153 0.0217 0.0044 0.0751 0.0244 0.0066 0.0559 0.1285
MMAC-SF 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0751 0.4739 0.0059 0.0082 0.0123 0.0639 0.0680 0.0236 0.4831 0.0262 0.0319 0.0279 0.0127 0.0206 0.0057 0.0845 0.0273 0.0081 0.0591 0.1988
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0707 0.6623 0.0063 0.0094 0.0166 0.1004 0.1040 0.0337 0.4630 0.0456 0.0384 0.0311 0.0183 0.0268 0.0094 0.1153 0.0352 0.0077 0.0645 0.2315
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0787 0.8850 0.0067 0.0071 0.0168 0.1086 0.0934 0.0300 0.4367 0.0473 0.0356 0.0376 0.0190 0.0378 0.0056 0.1253 0.0278 0.0084 0.0778 0.1758
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0597 0.5525 0.0039 0.0073 0.0123 0.0623 0.0691 0.0216 0.3049 0.0330 0.0287 0.0265 0.0178 0.0212 0.0069 0.0870 0.0251 0.0076 0.0531 0.1567
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0635 0.5780 0.0044 0.0063 0.0117 0.0712 0.0712 0.0206 0.4878 0.0325 0.0269 0.0319 0.0122 0.0261 0.0054 0.0958 0.0233 0.0078 0.0651 0.1486
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0822 0.6757 0.0096 0.0059 0.0159 0.1208 0.1236 0.0391 0.4149 0.0434 0.0419 0.0358 0.0202 0.0342 0.0107 0.1479 0.0366 0.0096 0.0893 0.2841
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0868 0.7813 0.0068 0.0094 0.0168 0.1269 0.1195 0.0378 0.4717 0.0450 0.0404 0.0476 0.0153 0.0446 0.0072 0.1575 0.0363 0.0103 0.1037 0.2049
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0513 0.4808 0.0057 0.0045 0.0107 0.0506 0.0518 0.0213 0.2801 0.0284 0.0290 0.0251 0.0154 0.0184 0.0039 0.0679 0.0205 0.0078 0.0623 0.1266
MMAC-SF 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0515 0.4444 0.0047 0.0069 0.0121 0.0524 0.0640 0.0205 0.4367 0.0277 0.0226 0.0276 0.0105 0.0198 0.0049 0.0795 0.0255 0.0126 0.0712 0.1616
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0687 0.6135 0.0072 0.0082 0.0132 0.0950 0.1182 0.0363 0.3731 0.0377 0.0483 0.0306 0.0158 0.0281 0.0103 0.1193 0.0291 0.0108 0.0678 0.1695
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0797 0.0055 0.0091 0.0150 0.1099 0.0897 0.0433 0.5465 0.0356 0.0389 0.0351 0.0181 0.0353 0.0049 0.1328 0.0241 0.0086 0.0785 0.1397
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0507 0.5848 0.0055 0.0056 0.0098 0.0573 0.0513 0.0243 0.2985 0.0220 0.0303 0.0316 0.0113 0.0196 0.0056 0.0797 0.0201 0.0062 0.0515 0.1068
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0509 0.5780 0.0076 0.0142 0.0683 0.0610 0.0202 0.4425 0.0236 0.0601 0.0363 0.0141 0.0193 0.0054 0.0802 0.0233 0.0086 0.0716 0.1178
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0698 0.5155 0.0064 0.0067 0.0123 0.0875 0.0931 0.0313 0.2899 0.0289 0.0477 0.0291 0.0157 0.0272 0.0088 0.1095 0.0272 0.0068 0.0812 0.1675
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0696 0.6250 0.0051 0.0089 0.0148 0.1264 0.0784 0.0325 0.4608 0.0315 0.0335 0.0415 0.0132 0.0321 0.0061 0.1224 0.0251 0.0073 0.1013 0.1570
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0504 0.4546 0.0033 0.0041 0.0093 0.0505 0.0387 0.0190 0.2924 0.0227 0.0369 0.0244 0.0125 0.0174 0.0042 0.0675 0.0180 0.0058 0.0625 0.0883
MMAC-SF 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0475 0.4310 0.0049 0.0089 0.0127 0.0561 0.0589 0.0175 0.4566 0.0213 0.0212 0.0261 0.0096 0.0176 0.0048 0.0755 0.0232 0.0066 0.0706 0.1488
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0498 0.4926 0.0041 0.0079 0.0106 0.0545 0.0603 0.0214 0.3247 0.0286 0.0305 0.0245 0.0106 0.0204 0.0065 0.0697 0.0217 0.0077 0.0466 0.1678
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0561 0.5780 0.0069 0.0048 0.0106 0.0669 0.0446 0.0221 0.4115 0.0266 0.0300 0.0250 0.0132 0.0232 0.0040 0.0814 0.0220 0.0059 0.0498 0.1100
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0545 0.5376 0.0036 0.0049 0.0103 0.0547 0.0505 0.0209 0.3413 0.0238 0.0353 0.0249 0.0115 0.0201 0.0058 0.0698 0.0203 0.0065 0.0515 0.1134
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0525 0.5465 0.0063 0.0062 0.0114 0.0536 0.0563 0.0215 0.4831 0.0245 0.0246 0.0252 0.0108 0.0187 0.0044 0.0738 0.0223 0.0080 0.0614 0.1203
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0503 0.4310 0.0037 0.0064 0.0104 0.0536 0.0463 0.0206 0.2584 0.0245 0.0318 0.0252 0.0121 0.0193 0.0060 0.0646 0.0203 0.0067 0.0445 0.1592
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0497 0.6329 0.0038 0.0053 0.0104 0.0553 0.0458 0.0199 0.4016 0.0224 0.0234 0.0257 0.0128 0.0231 0.0040 0.0727 0.0199 0.0058 0.0512 0.1082
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0770 0.7407 0.0045 0.0061 0.0140 0.0905 0.0716 0.0306 0.4255 0.0230 0.0349 0.0124 0.0308 0.0073 0.1362 0.0239 0.0099 0.0870 0.2778