Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4386 1.0776 0.3003 0.2625 0.1880 0.3891 0.3247 1.1547 0.4000 0.4329 0.9174 0.4695 0.5882 0.1361 0.2481 1.1779 0.3268
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4219 1.0331 0.3068 0.2762 0.2137 0.4065 0.3205 1.1779 0.4000 0.4546 0.9009 0.3846 0.5848 0.1445 0.2326 1.1862 0.3356
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4098 1.0858 0.2770 0.2959 0.2193 0.4310 0.3401 1.1779 0.4202 0.4525 0.9524 0.4386 0.6250 0.1468 0.2481 1.1013 0.3534
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5263 1.0977 0.3345 0.3096 0.2222 0.4505 0.3436 1.2136 0.4566 0.4739 0.9615 0.4184 0.6098 0.1486 0.2404 1.1641 0.3677
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.3937 1.1261 0.2809 0.2841 0.2000 0.4237 0.3106 1.1198 0.3759 0.4831 0.9174 0.4329 0.5435 0.1403 0.2404 1.1481 0.3610
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4425 1.1186 0.3846 0.2941 0.2128 0.4329 0.3040 1.1601 0.3509 0.4739 0.9434 0.4115 0.5376 0.1684 0.2342 1.1820 0.3509
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.3937 0.9901 0.2933 0.3077 0.2310 0.4065 0.3205 1.1468 0.3413 0.4717 0.9259 0.4167 0.5587 0.1376 0.2639 1.0081 0.3610
LOXIMVI 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4651 1.0070 0.3215 0.3300 0.2310 0.4219 0.3175 1.1442 0.3425 0.4425 0.9346 0.4132 0.5181 0.1616 0.2584 1.0858 0.3534
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.4630 1.1186 0.2604 0.2841 0.2079 0.4329 0.3322 1.1737 0.4310 0.4902 0.3968 0.6289 0.1425 0.2387 1.2121 0.3610
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5051 1.1416 0.3003 0.2778 0.2237 0.4630 0.3125 1.1338 0.4386 0.4926 0.9524 0.3937 0.6135 0.1364 0.2141 1.2151 0.3559
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.4525 1.1173 0.2681 0.2778 0.1996 0.4292 0.3257 1.1669 0.4348 0.4695 0.9346 0.3937 0.6329 0.1409 0.2494 1.1111 0.3509
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.4878 1.0707 0.3125 0.2899 0.2188 0.4546 0.3300 1.1468 0.4484 0.4202 0.9346 0.4032 0.5882 0.1445 0.2445 1.1806 0.3663
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.3984 1.1976 0.2625 0.2597 0.2092 0.4255 0.3058 1.1723 0.3677 0.4386 0.9346 0.3891 0.5291 0.1189 0.2288 1.1494 0.3333
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.3817 1.0858 0.2899 0.2681 0.2110 0.4444 0.3058 1.1261 0.3731 0.3968 0.9091 0.3718 0.5076 0.1414 0.2151 1.1919 0.3268
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.4132 0.9901 0.2571 0.2857 0.2037 0.4016 0.2786 1.1710 0.3247 0.4329 0.9259 0.3663 0.5025 0.1103 0.2571 1.1148 0.3345
LOXIMVI 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.4065 0.9615 0.3040 0.2653 0.2165 0.4184 0.2825 1.1403 0.3497 0.4098 0.9524 0.3937 0.4484 0.1348 0.2336 1.1507 0.3571
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4255 1.1779 0.2865 0.2865 0.2146 0.4292 0.3125 1.2484 0.4367 0.4673 0.9346 0.3906 0.5988 0.1401 0.2577 1.1710 0.3650
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4132 1.1429 0.3125 0.2786 0.2128 0.4546 0.3040 1.2270 0.4202 0.4505 0.9346 0.3906 0.6024 0.1033 0.2347 1.2180 0.3345
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4739 1.0593 0.2933 0.3145 0.2045 0.3937 0.3484 1.1848 0.3876 0.4546 0.8772 0.4292 0.6369 0.1253 0.2551 1.0363 0.3509
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.5236 1.0246 0.3268 0.2933 0.2268 0.4310 0.3484 1.1976 0.4032 0.4237 0.8929 0.4115 0.6024 0.1294 0.2310 1.0834 0.3690
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4149 1.1136 0.2770 0.2778 0.2101 0.4237 0.3322 1.1390 0.3636 0.4546 0.9259 0.4065 0.5208 0.1379 0.2532 1.1198 0.3448
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4098 1.0707 0.3247 0.2703 0.2198 0.4444 0.3086 1.1641 0.3731 0.4082 0.9009 0.3953 0.4808 0.1445 0.2174 1.1429 0.3460
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4854 1.0395 0.2817 0.3279 0.2212 0.4167 0.3049 1.0941 0.3247 0.4608 0.9009 0.3968 0.5291 0.1163 0.2717 1.0331 0.3610
LOXIMVI 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.4651 0.9709 0.3165 0.3086 0.4149 0.3215 1.1274 0.3436 0.4184 0.8850 0.3984 0.4854 0.1311 0.2710 1.1325 0.3704
LOXIMVI 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.4484 1.2034 0.2933 0.2994 0.2165 0.4673 0.4386 1.1792 0.5155 0.4717 0.9709 0.4444 0.6623 0.1484 0.2404 1.1933 0.3745