Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4367 1.1494 0.3021 0.2994 0.2066 0.4484 0.3677 1.3316 0.4386 0.4464 1.0384 0.4329 0.5587 0.1421 1.2121 0.3322
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.3718 1.2092 0.2597 0.2710 0.1976 0.4546 0.3817 1.3423 0.3571 0.4673 1.0183 0.4132 0.5181 0.2203 1.1641 0.3290
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4237 1.1601 0.2762 0.2591 0.2137 0.4566 0.3413 1.3351 0.3497 0.4348 0.9901 0.4167 0.5128 0.1282 0.2188 1.1806 0.3215
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.3906 1.1211 0.2695 0.2874 0.2101 0.4132 0.3677 1.2361 0.3195 0.4425 1.0215 0.4049 0.5025 0.1193 0.2488 1.0858 0.3175
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.3650 1.0799 0.3205 0.2674 0.2217 0.4184 0.3677 1.3123 0.3472 0.3906 0.9901 0.4065 0.4975 0.1211 0.2416 1.1287
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4184 1.1669 0.2551 0.2653 0.2020 0.4237 0.3436 1.3717 0.4065 0.4525 1.0471 0.4386 0.5882 0.1227 0.2375 1.2165 0.3226
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.3984 1.1299 0.2967 0.2653 0.1984 0.4367 0.3597 1.3369 0.4184 0.4367 1.0173 0.4115 0.6211 0.1217 1.2063 0.3247
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4132 1.1299 0.2667 0.2703 0.1832 0.3831 0.3290 1.2438 0.3559 0.4237 0.9804 0.4032 0.5952 0.1146 0.2625 1.0718 0.3135
LOXIMVI 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4237 1.0428 0.2950 0.2793 0.1961 0.3774 0.3718 1.2739 0.3788 0.3906 1.0050 0.4255 0.5291 0.1309 0.2310 1.1442 0.3215
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4167 1.1891 0.2833 0.2916 0.2242 0.3718 0.4049 1.3210 0.2778 0.4464 0.9524 0.4202 0.5465 0.1284 0.2703 1.0111 0.3333
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4444 1.1723 0.2762 0.2924 0.2222 0.3677 0.3876 1.2165 0.3012 0.4082 0.9346 0.3922 0.5319 0.1221 0.2433 1.0352 0.3311
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4167 1.1641 0.2688 0.3058 0.2410 0.3937 0.4274 1.2579 0.2865 0.4566 0.9259 0.4184 0.5465 0.1218 0.2933 0.8772 0.3484
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4546 1.1976 0.3175 0.3049 0.2481 0.4032 0.4464 1.2937 0.2967 0.4255 0.9091 0.4149 0.5405 0.1193 0.2778 0.9009 0.3677
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4525 1.1534 0.2364 0.2778 0.1957 0.3802 0.3636 1.3004 0.4132 0.4405 1.0000 0.4525 0.5882 0.2410 1.0449 0.3401
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4049 1.1236 0.2500 0.2976 0.2137 0.3745 0.3953 1.2376 0.4167 0.4065 0.9709 0.4348 0.5682 0.1188 0.2183 1.0953 0.3247
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4762 1.0905 0.2632 0.3215 0.2347 0.4032 0.4167 1.3245 0.3831 0.4717 0.9346 0.4329 0.6289 0.1199 0.2890 0.9434 0.3460
LOXIMVI 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4651 1.1211 0.2825 0.3012 0.2381 0.4082 0.4566 1.3106 0.3968 0.4405 0.9091 0.4219 0.6024 0.2865 0.9615 0.3584
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4274 1.1274 0.3135 0.3058 0.2033 0.4484 0.4098 1.2270 0.4739 0.4630 0.9709 0.4484 0.6536 0.1495 0.2451 1.1905 0.3663
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4785 1.1338 0.3125 0.3311 0.2242 0.4608 0.3876 1.2195 0.4926 0.4878 1.0000 0.4405 0.6757 0.1280 0.2577 1.2346 0.3759
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4739 1.0764 0.3030 0.3677 0.2247 0.4292 0.4082 1.1806 0.4329 0.4785 0.9346 0.4237 0.6494 0.1499 0.2688 1.0965 0.3774
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5405 1.1186 0.4000 0.2320 0.4405 0.4219 1.2531 0.4348 0.5181 0.9615 0.4762 0.6452 0.1577 0.2732 1.1173 0.3922
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.3968 1.0834 0.2519 0.3155 0.1988 0.4274 0.3290 1.2063 0.3460 0.4630 0.9615 0.4367 0.5051 0.1372 0.2674 1.1574 0.3559
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4505 1.0917 0.2732 0.3247 0.2165 0.4425 0.3390 1.2180 0.3401 0.4464 0.9259 0.4098 0.5263 0.1439 0.2584 1.1876 0.3663
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4505 1.0225 0.3030 0.3677 0.2433 0.4310 0.3677 1.1628 0.3460 0.4831 0.9259 0.4505 0.5587 0.1493 0.2849 1.0684 0.3731
LOXIMVI 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4902 1.0173 0.3021 0.3718 0.2500 0.4546 0.4000 0.3472 0.4926 0.9346 0.4630 0.5263 0.1458 0.2755 1.1013 0.3759