Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.2994 1.3423 0.2278 0.2475 0.1980 0.4255 0.3731 1.5699 0.3610 0.4525 1.2107 0.4032 0.4464 0.1217 0.2653 1.1001 0.2710
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3401 1.4225 0.2513 0.2551 0.2232 0.4292 0.3597 1.6103 0.3509 0.4785 1.1891 0.4255 0.4739 0.1101 0.2375 1.0893 0.2976
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3257 1.1561 0.2439 0.2632 0.2237 0.4167 0.3401 1.3928 0.3205 0.4405 1.0811 0.4098 0.4367 0.1235 0.2304 1.1628 0.2809
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3774 1.1792 0.2825 0.2933 0.2410 0.4951 0.3817 1.4472 0.3356 0.4237 1.1641 0.4329 0.4348 0.1295 0.2392 1.2315 0.3185
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3145 1.2706 0.2667 0.2433 0.1961 0.4032 0.3597 1.4535 0.3509 0.4115 1.0661 0.3704 0.3546 0.1381 0.2114 1.1601 0.2611
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3165 1.2516 0.2778 0.2299 0.3906 0.3448 1.4327 0.3521 0.4525 1.0881 0.3718 0.4065 0.1139 0.2041 1.0905 0.2703
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3096 1.2469 0.2188 0.2342 0.2123 0.4000 0.3731 1.3333 0.3279 0.4065 1.0549 0.3984 0.4049 0.1124 0.1976 1.0204 0.2907
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3891 1.2034 0.2809 0.2370 0.2278 0.3345 1.3947 0.3677 0.4098 1.0537 0.4219 0.3984 0.1326 0.2020 1.0560 0.2985
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3030 1.3210 0.2299 0.2375 0.2310 0.4132 0.3690 1.4245 0.3436 0.4310 1.1429 0.4149 0.4546 0.1269 0.2049 1.0917 0.2809
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3623 1.2658 0.2639 0.2331 0.2299 0.4255 0.3448 1.4025 0.3650 0.4484 1.1111 0.3831 0.4566 0.1381 0.2088 1.0593 0.2907
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3185 1.0173 0.2500 0.2392 0.2165 0.4082 0.3300 1.2903 0.3236 0.4000 1.0893 0.3906 0.4444 0.1244 0.2128 1.0000 0.2793
LOXIMVI 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.3436 1.1123 0.2890 0.2326 0.4386 0.3356 1.3459 0.3236 0.4219 1.1099 0.3846 0.4049 0.1357 0.2062 1.0684 0.2976
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.2994 1.3405 0.2310 0.2212 0.2288 0.4219 1.3699 0.3509 0.4630 1.0571 0.3846 0.3922 0.1263 0.2252 1.1074 0.2874
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3300 1.3514 0.2551 0.2525 0.2273 0.4237 0.3356 1.3624 0.3861 0.4202 1.0799 0.3731 0.4310 0.1175 0.1934 1.0449 0.2801
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3300 1.2240 0.2646 0.2457 0.2037 0.3968 0.3247 1.3477 0.3175 0.4115 1.0549 0.4032 0.4032 0.1155 0.2331 1.0787 0.2907
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.2916 1.1561 0.2762 0.2342 0.2227 0.4167 0.3425 1.3316 0.3311 0.3497 1.0799 0.4082 0.3731 0.1131 0.1953 1.1574 0.2950
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3195 1.3441 0.2137 0.2404 0.2155 0.4149 0.3401 1.3870 0.3279 0.4386 1.0989 0.3663 0.4367 0.1043 0.2160 1.0341 0.2762
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3165 1.2453 0.2439 0.2410 0.2336 0.3460 1.3928 0.3610 0.3876 1.0741 0.4049 0.4425 0.1020 0.2045 1.0846 0.2747
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3401 1.0764 0.2227 0.2304 0.2033 0.4049 0.3185 1.3245 0.2976 0.4098 1.0787 0.4202 0.4329 0.1089 0.2208 1.0905 0.2833
LOXIMVI 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.3448 1.0331 0.2882 0.2481 0.2326 0.4000 0.3195 1.3298 0.3106 0.3623 1.0834 0.4000 0.3636 0.1269 0.1961 1.1574 0.3021
LOXIMVI 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.3546 1.3477 0.2571 0.2817 0.2488 0.4132 0.3817 1.5038 0.3690 1.0549 0.4167 0.4065 0.1185 0.2262 1.0650 0.2933
LOXIMVI 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.3831 1.3532 0.2392 0.2801 0.2353 0.4386 0.3731 1.4144 0.3891 0.3984 1.0060 0.3922 0.3953 0.1152 0.2212 1.0893 0.2994
LOXIMVI 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 1.0977 0.2427 0.2874 0.2160 0.3650 0.3677 1.3245 0.2916 0.4132 0.9524 0.4098 0.4149 0.1025 0.2445 0.9804 0.2874
LOXIMVI 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.3802 1.1074 0.2907 0.2611 0.2304 0.3677 0.3891 1.3004 0.2976 0.3891 0.9524 0.3906 0.4049 0.1098 0.2283 1.0215 0.3155
LOXIMVI 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.3802 1.1123 0.2421 0.2778 0.2347 0.3546 0.3484 1.3405 0.2770 0.4274 0.8929 0.4000 0.4405 0.1236 0.2278 0.8696 0.3145