Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3610 1.3459 0.2004 0.2439 0.2045 0.4167 0.2755 1.3387 0.4115 0.4505 1.0764 0.4115 0.5495 0.1418 0.2747 0.9174 0.2865
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3058 1.2005 0.2375 0.2242 0.2227 0.4255 0.2976 1.3123 0.4274 0.3861 1.1001 0.4202 0.5319 0.1458 0.2079 0.9346 0.2786
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3322 1.5221 0.2278 0.2242 0.2105 0.4386 0.3125 1.4493 0.3774 0.4566 1.1547 0.4049 0.5051 0.1083 0.2398 0.8475 0.2809
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3226 1.3850 0.2203 0.2174 0.2392 0.4386 0.3115 1.4472 0.4098 0.4219 1.1468 0.4202 0.5051 0.1208 0.1969 0.8772 0.2899
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3448 1.1547 0.2283 0.2359 0.1938 0.3597 0.3135 1.4124 0.3356 0.4202 1.1111 0.5051 0.1018 0.2222 0.8696 0.2646
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3636 1.1682 0.2525 0.2387 0.2174 0.3906 0.2899 1.3870 0.3846 0.3731 1.1198 0.4202 0.1261 0.2110 0.8850 0.3012
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3759 1.2315 0.2217 0.2451 0.2000 0.3906 0.2976 1.3569 0.3817 0.4149 1.1050 0.4329 0.5155 0.1309 0.2331 0.8696 0.3003
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.4049 1.1312 0.2208 0.2519 0.2155 0.3937 0.2882 1.3717 0.3831 0.3677 1.0384 0.3968 0.5051 0.1238 0.2066 0.8130 0.3049
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3584 1.1325 0.2639 0.1992 0.3663 0.2924 1.2136 0.3460 0.4149 0.9804 0.4132 0.5181 0.1094 0.2288 0.7874 0.2786
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3891 1.1111 0.2247 0.2392 0.2193 0.3597 0.3012 1.2594 0.3677 0.3610 1.0000 0.4132 0.4673 0.1036 0.2198 0.8065 0.3096
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3718 1.1876 0.2049 0.2457 0.2268 0.3068 1.2903 0.3290 0.4219 1.0627 0.4274 0.4951 0.1082 0.2353 0.8772 0.2959
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3559 1.1364 0.2169 0.2481 0.2326 0.3984 0.3175 1.2500 0.3401 0.3861 1.0373 0.3937 0.4717 0.1242 0.2222 0.8547 0.2916
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3831 1.0858 0.2227 0.2933 0.2268 0.3759 0.3030 1.2788 0.3125 0.4184 1.0299 0.3817 0.5102 0.1138 0.2398 0.8475 0.2817
LOXIMVI 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.3774 1.1442 0.2770 0.2433 0.3906 0.3215 1.2579 0.3311 0.3817 1.0173 0.3968 0.4785 0.1186 0.2165 0.8696 0.2688
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3115 1.2723 0.2564 0.2392 0.2315 0.4274 0.3636 1.4430 0.3584 0.4566 1.1429 0.4219 0.4082 0.1575 0.2151 1.1287 0.2857
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3802 1.2920 0.2717 0.2674 0.2421 0.4132 0.3610 1.4815 0.3663 0.4762 1.1442 0.4386 0.4464 0.1211 0.2155 1.1198 0.2907
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3333 1.1274 0.2326 0.2809 0.2283 0.4255 0.3846 1.3333 0.3185 0.4386 1.0235 0.4115 0.4274 0.1355 0.2294 1.0672 0.2841
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.4032 1.2107 0.2717 0.2985 0.2299 0.4202 0.3861 1.4025 0.3125 0.4525 1.0941 0.4292 0.4149 0.1475 0.2331 1.0695 0.3003
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3058 1.1211 0.2445 0.2494 0.1931 0.4292 0.3546 1.5015 0.3571 0.4065 1.1601 0.4132 0.4237 0.1233 0.2033 1.0858
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3597 1.1737 0.2639 0.2488 0.2132 0.4184 0.3861 1.4881 0.3774 0.4444 1.1820 0.4049 0.4310 0.1245 0.2278 1.1038 0.2959
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3290 1.1161 0.2288 0.2865 0.2058 0.3610 0.3546 1.3624 0.3012 0.4310 1.0917 0.4202 0.4444 0.1215 0.2310 1.0235 0.2933
LOXIMVI 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.3906 1.1561 0.2558 0.3040 0.2198 0.3731 0.3597 1.3495 0.3030 0.4274 1.1377 0.4098 0.4255 0.1311 0.2427 1.0256 0.3012
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.3012 1.3106 0.2433 0.2137 0.1890 0.4255 1.4706 0.3311 0.4310 1.1862 0.3817 0.3731 0.1079 0.1923 1.0571 0.2817
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.2857 1.3280 0.2481 0.2110 0.2114 0.4000 1.4205 0.3521 0.4098 1.1148 0.3559 0.3922 0.1167 0.1946 1.0152 0.2703
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.2950 1.1403 0.2114 0.2347 0.2037 0.4082 0.3534 1.3831 0.3205 0.4219 1.0799 0.4000 0.3968 0.1083 0.2096 1.0428 0.2717