Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3906 1.4771 0.2404 0.2451 0.1880 0.4329 0.3049 1.4286 0.4292 1.0905 0.4329 0.4902 0.1188 0.2160 1.0040 0.3125
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3937 1.2092 0.2141 0.2320 0.2070 0.3968 0.2770 1.4347 0.3953 0.4219 1.0593 0.4237 0.5000 0.1183 0.2146 0.8696 0.2825
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3876 1.2270 0.2237 0.2326 0.2252 0.3922 0.2809 0.3968 0.3704 1.0846 0.4167 0.4608 0.1072 0.2252 0.8621 0.2833
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3690 1.5015 0.2000 0.2252 0.2433 0.3690 0.3247 1.4451 0.4065 0.4630 1.1962 0.4425 0.4808 0.1201 0.2273 0.9091 0.2941
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3571 1.6181 0.2331 0.2463 0.2551 0.4348 0.3058 1.3870 0.4016 0.4184 1.1223 0.4184 0.4785 0.2137 0.8621 0.3040
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3650 1.2315 0.2257 0.2416 0.2155 0.4219 0.3086 1.4948 0.3788 0.4484 1.1507 0.4219 0.5376 0.1239 0.2273 0.9009 0.2916
LOXIMVI 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3846 1.2195 0.2469 0.2551 0.2747 0.4132 0.3300 1.4368 0.3953 0.3984 1.1669 0.4184 0.4695 0.1120 0.2525 0.8403 0.3058
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3155 1.4749 0.2591 0.2439 0.1859 0.4237 0.2857 1.4184 0.4546 0.4274 1.2225 0.4673 0.5376 0.1520 0.2506 1.0183 0.3175
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3831 1.4085 0.2494 0.2451 0.1931 0.4525 0.2667 1.4472 0.4831 0.5076 1.2210 0.4717 0.5525 0.1124 0.2398 1.0449 0.3155
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3390 1.1976 0.2268 0.2381 0.1808 0.3937 0.2882 1.3532 0.4032 0.4348 1.0823 0.4444 0.5102 0.1174 0.2278 0.8850 0.2882
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4202 1.3021 0.2310 0.2747 0.2105 0.4237 0.2967 1.3514 0.4310 0.4525 1.1173 0.4673 0.5051 0.1120 0.2315 0.9091 0.3068
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3759 1.6529 0.2227 0.2591 0.2320 0.3846 0.2882 1.5015 0.4132 0.4785 1.2626 0.4630 0.5102 0.1355 0.9009 0.2985
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3597 1.6502 0.2174 0.2959 0.2037 0.3984 0.3356 1.5699 0.4184 0.5102 1.1962 0.4425 0.5155 0.1264 0.2381 0.8696 0.3311
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3215 1.3405 0.2597 0.2639 0.2310 0.4329 0.3236 1.4184 0.4082 0.4651 1.2034 0.4525 0.5405 0.1300 0.2392 0.8065 0.2882
LOXIMVI 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.3984 1.3699 0.2882 0.2778 0.2481 0.4444 0.3390 1.4903 0.4484 0.4717 1.2195 0.4608 0.5181 0.1445 0.7813 0.3165
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.2841 1.4045 0.2049 0.2331 0.1721 0.3802 0.2809 1.3680 0.4464 0.4367 1.1628 0.4065 0.4608 0.1405 0.2320 0.9804 0.2967
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3003 1.3193 0.2083 0.2387 0.1934 0.3984 0.2688 1.3793 0.4202 0.4292 1.1416 0.4132 0.4854 0.1323 0.2247 0.9434 0.3125
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3268 1.3928 0.1923 0.2278 0.2096 0.3891 0.2825 1.4430 0.4219 0.4386 1.1161 0.4237 0.5236 0.1153 0.2237 0.8850 0.2933
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2920 0.2398 0.2304 0.1876 0.4292 0.2747 1.3699 0.4673 0.4082 1.1223 0.4505 0.4926 0.1429 0.2083 0.9174 0.2916
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3096 1.5699 0.2347 0.2198 0.2179 0.3906 0.3030 1.4225 0.4348 1.1862 0.4000 0.5181 0.1353 0.2359 0.9434 0.3086
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3165 1.4706 0.2457 0.2525 0.2160 0.4132 0.3030 1.4025 0.4115 0.4274 1.1481 0.4274 0.5181 0.1401 0.2174 0.9524 0.2793
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3049 1.2407 0.2155 0.2427 0.2020 0.3968 0.3175 1.3889 0.3623 0.4405 1.0989 0.4032 0.5376 0.1115 0.2012 0.8197 0.2899
LOXIMVI 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.3279 1.2270 0.2532 0.2387 0.2439 0.4525 0.3040 1.4104 0.4065 0.4219 1.1751 0.4049 0.4695 0.1351 0.2183 0.8547 0.3030
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3390 1.3755 0.2494 0.2463 0.2083 0.4016 0.2865 1.3661 0.4405 0.4237 1.1403 0.4255 0.5128 0.1421 0.2584 0.9346 0.2967
LOXIMVI 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.3497 1.2987 0.2445 0.2639 0.2119 0.4115 0.2933 1.3123 0.4444 0.4184 1.0515 0.4386 0.5181 0.1264 0.2232 0.9091 0.2994