Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0776 0.1070 0.0535 0.0055 0.0140 0.0256 0.0026 0.0034 0.0338 0.0123 0.0239 0.1057 0.0136 0.0112 0.0038 0.0467 0.0091 0.0103 0.0938 0.0559
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0722 0.0885 0.0627 0.0068 0.0243 0.0293 0.0036 0.0042 0.0285 0.0198 0.0258 0.1161 0.0145 0.0107 0.0047 0.0482 0.0159 0.0075 0.0895 0.0664
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0835 0.1645 0.0833 0.0069 0.0310 0.0347 0.0044 0.0066 0.0458 0.0202 0.0457 0.1689 0.0183 0.0194 0.0045 0.0618 0.0175 0.0107 0.2481 0.0710
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0877 0.1511 0.0853 0.0069 0.0211 0.0312 0.0046 0.0072 0.0452 0.0192 0.0408 0.1527 0.0162 0.0208 0.0046 0.0618 0.0170 0.0121 0.2053 0.0602
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0715 0.1040 0.0703 0.0063 0.0188 0.0283 0.0027 0.0037 0.0359 0.0130 0.0315 0.1285 0.0129 0.0122 0.0035 0.0485 0.0105 0.0087 0.1667 0.0590
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0749 0.0987 0.0713 0.0067 0.0192 0.0243 0.0033 0.0054 0.0335 0.0133 0.0310 0.1299 0.0122 0.0138 0.0048 0.0512 0.0120 0.0068 0.1239 0.0628
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0929 0.2294 0.0932 0.0085 0.0239 0.0477 0.0063 0.0079 0.0745 0.0280 0.0499 0.2037 0.0217 0.0253 0.0053 0.0668 0.0203 0.0117 0.3322 0.0806
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0995 0.2004 0.0928 0.0101 0.0239 0.0384 0.0057 0.0111 0.0704 0.0276 0.0478 0.1908 0.0214 0.0271 0.0074 0.0707 0.0211 0.0083 0.2681 0.0722
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0717 0.1127 0.0631 0.0062 0.0147 0.0269 0.0024 0.0032 0.0430 0.0143 0.0263 0.1181 0.0142 0.0115 0.0032 0.0464 0.0088 0.0074 0.1832 0.0467
LOXIMVI 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0776 0.1110 0.0659 0.0115 0.0154 0.0265 0.0035 0.0053 0.0372 0.0160 0.0259 0.1355 0.0137 0.0127 0.0056 0.0487 0.0141 0.0073 0.1376 0.0583
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0912 0.1359 0.0905 0.0084 0.0265 0.0351 0.0036 0.0063 0.0525 0.0190 0.0405 0.1946 0.0171 0.0192 0.0041 0.0630 0.0150 0.0109 0.2907 0.1020
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1007 0.1341 0.0859 0.0070 0.0247 0.0282 0.0041 0.0072 0.0474 0.0165 0.0392 0.1669 0.0157 0.0197 0.0044 0.0583 0.0163 0.0101 0.2703 0.0652
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0840 0.1174 0.0741 0.0085 0.0212 0.0286 0.0029 0.0045 0.0473 0.0130 0.0375 0.1684 0.0130 0.0172 0.0039 0.0513 0.0133 0.0096 0.1905 0.0698
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0823 0.1100 0.0697 0.0089 0.0220 0.0227 0.0036 0.0060 0.0398 0.0135 0.0348 0.1416 0.0126 0.0147 0.0056 0.0509 0.0151 0.0093 0.1815 0.0552
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0834 0.1758 0.0882 0.0087 0.0227 0.0367 0.0041 0.0066 0.0582 0.0212 0.0459 0.2083 0.0197 0.0245 0.0044 0.0583 0.0197 0.0124 0.2786 0.0965
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0912 0.1555 0.0842 0.0084 0.0235 0.0298 0.0049 0.0086 0.0514 0.0211 0.0448 0.1689 0.0169 0.0211 0.0049 0.0594 0.0187 0.0095 0.2793 0.0631
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0770 0.1004 0.0633 0.0068 0.0166 0.0277 0.0025 0.0043 0.0605 0.0126 0.0267 0.1364 0.0135 0.0146 0.0035 0.0476 0.0099 0.0081 0.2075 0.0494
LOXIMVI 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0870 0.0950 0.0625 0.0074 0.0166 0.0224 0.0033 0.0059 0.0426 0.0125 0.0282 0.1316 0.0138 0.0127 0.0046 0.0487 0.0146 0.0089 0.1869 0.0484
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1088 0.1502 0.0780 0.0073 0.0225 0.0400 0.0053 0.0058 0.0352 0.0207 0.0432 0.1912 0.0222 0.0185 0.0056 0.0616 0.0226 0.0093 0.1890 0.1271
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1152 0.1637 0.0847 0.0073 0.0231 0.0351 0.0059 0.0086 0.0368 0.0229 0.0466 0.1795 0.0216 0.0217 0.0070 0.0637 0.0213 0.0115 0.1972 0.1002
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1037 0.1057 0.0726 0.0067 0.0186 0.0335 0.0037 0.0365 0.0142 0.0323 0.1502 0.0181 0.0167 0.0056 0.0552 0.0128 0.0085 0.1727 0.0992
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1098 0.1166 0.0773 0.0068 0.0237 0.0275 0.0038 0.0062 0.0368 0.0158 0.0344 0.1603 0.0174 0.0149 0.0059 0.0629 0.0143 0.0087 0.1695 0.0841
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1170 0.1767 0.0882 0.0087 0.0203 0.0480 0.0054 0.0063 0.0502 0.0228 0.0524 0.2101 0.0235 0.0163 0.0071 0.0611 0.0191 0.0106 0.2667 0.1409
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1236 0.1852 0.0906 0.0080 0.0251 0.0389 0.0053 0.0083 0.0535 0.0268 0.0537 0.2193 0.0225 0.0180 0.0081 0.0654 0.0221 0.0122 0.2778 0.1163
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0945 0.0784 0.0568 0.0065 0.0152 0.0313 0.0038 0.0043 0.0433 0.0138 0.0219 0.1529 0.0178 0.0137 0.0041 0.0521 0.0110 0.0078 0.1712 0.0853