Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1036 0.2203 0.1149 0.0115 0.0141 0.0365 0.0047 0.0120 0.4717 0.0197 0.0505 0.3012 0.0282 0.0306 0.0055 0.0758 0.0187 0.0086 0.4115 0.0691
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0755 0.1339 0.0751 0.0082 0.0113 0.0332 0.0026 0.0046 0.4000 0.0121 0.0295 0.2320 0.0228 0.0172 0.0036 0.0593 0.0101 0.0074 0.2778 0.0508
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0800 0.1196 0.0855 0.0087 0.0109 0.0245 0.0036 0.0074 0.3175 0.0113 0.0299 0.2331 0.0225 0.0195 0.0046 0.0559 0.0137 0.0065 0.2278 0.0563
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1019 0.1344 0.0855 0.0110 0.0135 0.0391 0.0028 0.0084 0.5435 0.0266 0.0251 0.2841 0.0301 0.0354 0.0033 0.0759 0.0113 0.0079 0.2747 0.1034
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1099 0.1205 0.0777 0.0099 0.0113 0.0282 0.0028 0.0071 0.5291 0.0238 0.0290 0.2488 0.0265 0.0425 0.0034 0.0646 0.0100 0.0078 0.2967 0.0541
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0958 0.1595 0.0707 0.0114 0.0114 0.0303 0.0027 0.0054 0.5319 0.0251 0.0282 0.3205 0.0246 0.0442 0.0038 0.0576 0.0122 0.0075 0.2618 0.0985
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1042 0.1391 0.0685 0.0099 0.0121 0.0235 0.0033 0.0076 0.4464 0.0217 0.0267 0.2381 0.0246 0.0293 0.0039 0.0590 0.0124 0.0076 0.2597 0.0675
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0927 0.1961 0.1181 0.0133 0.0159 0.0357 0.0036 0.0085 0.5848 0.0163 0.0474 0.3623 0.0291 0.0281 0.0047 0.0702 0.0154 0.0091 0.4098 0.0956
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0986 0.1792 0.1125 0.0116 0.0142 0.0289 0.0042 0.0102 0.5263 0.0160 0.0456 0.2817 0.0254 0.0272 0.0053 0.0669 0.0172 0.0090 0.4049 0.0604
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0939 0.1146 0.0671 0.0092 0.0137 0.0327 0.0027 0.0054 0.4525 0.0119 0.0250 0.2762 0.0247 0.0265 0.0035 0.0663 0.0116 0.0071 0.2591 0.0806
LOXIMVI 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1059 0.1010 0.0697 0.0087 0.0143 0.0243 0.0042 0.0089 0.3195 0.0126 0.0230 0.2525 0.0246 0.0185 0.0039 0.0643 0.0115 0.0071 0.2545 0.0627
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1079 0.1590 0.0829 0.0079 0.0228 0.0444 0.0052 0.0071 0.0391 0.0199 0.0494 0.2016 0.0221 0.0200 0.0055 0.0627 0.0207 0.0102 0.2336 0.1153
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1120 0.1815 0.0874 0.0095 0.0243 0.0383 0.0070 0.0102 0.0427 0.0242 0.0519 0.2000 0.0219 0.0245 0.0075 0.0672 0.0242 0.0114 0.2416 0.0999
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0983 0.1192 0.0770 0.0087 0.0196 0.0368 0.0038 0.0046 0.0404 0.0200 0.0354 0.1541 0.0167 0.0150 0.0052 0.0545 0.0131 0.0091 0.1842 0.1034
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1047 0.1134 0.0824 0.0075 0.0298 0.0040 0.0071 0.0415 0.0149 0.0380 0.1642 0.0182 0.0153 0.0064 0.0670 0.0161 0.0091 0.1792 0.0840
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1093 0.1610 0.0832 0.0085 0.0178 0.0429 0.0044 0.0059 0.0475 0.0211 0.0428 0.1828 0.0202 0.0174 0.0064 0.0564 0.0157 0.0101 0.2398 0.1294
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1103 0.1595 0.0896 0.0078 0.0200 0.0328 0.0050 0.0081 0.0492 0.0236 0.0456 0.1912 0.0201 0.0172 0.0071 0.0603 0.0196 0.0106 0.2421 0.1020
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0973 0.0925 0.0588 0.0067 0.0144 0.0304 0.0035 0.0042 0.0399 0.0135 0.0237 0.1325 0.0165 0.0125 0.0041 0.0530 0.0097 0.0077 0.1595 0.0887
LOXIMVI 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1002 0.0858 0.0555 0.0068 0.0179 0.0301 0.0035 0.0046 0.0365 0.0152 0.0239 0.1385 0.0182 0.0116 0.0049 0.0587 0.0126 0.0087 0.1518 0.0687
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1062 0.2128 0.0865 0.0088 0.0257 0.0418 0.0053 0.0078 0.0465 0.0240 0.0529 0.1770 0.0200 0.0235 0.0054 0.0688 0.0211 0.0115 0.2801
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1010 0.2123 0.0884 0.0083 0.0258 0.0399 0.0056 0.0085 0.0532 0.0259 0.0552 0.1808 0.0214 0.0262 0.0057 0.0700 0.0210 0.0092 0.2212 0.0637
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0776 0.1050 0.0589 0.0059 0.0151 0.0258 0.0025 0.0040 0.0312 0.0118 0.0258 0.1138 0.0122 0.0116 0.0041 0.0467 0.0096 0.0076 0.1258 0.0611
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0762 0.0920 0.0630 0.0082 0.0166 0.0245 0.0030 0.0051 0.0324 0.0112 0.0263 0.1198 0.0146 0.0110 0.0043 0.0549 0.0103 0.0058 0.1020 0.0649
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0838 0.1748 0.0765 0.0067 0.0177 0.0356 0.0043 0.0059 0.0361 0.0184 0.0462 0.1488 0.0183 0.0187 0.0052 0.0537 0.0163 0.0103 0.1818 0.0784
LOXIMVI 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0799 0.1595 0.0732 0.0071 0.0182 0.0314 0.0049 0.0074 0.0381 0.0199 0.0456 0.1486 0.0194 0.0184 0.0058 0.0564 0.0166 0.0081 0.1409 0.0672