Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1027 0.7692 0.1027 0.0162 0.0273 0.0497 0.0084 0.0125 0.6536 0.0341 0.0993 0.4902 0.0590 0.0542 0.0096 0.0806 0.0299 0.0170 0.1595 0.1592
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0649 0.3145 0.0448 0.0089 0.0141 0.0273 0.0032 0.0043 0.4505 0.0157 0.0397 0.2667 0.0270 0.0261 0.0043 0.0446 0.0126 0.0091 0.0924 0.0943
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0661 0.3049 0.0538 0.0115 0.0145 0.0263 0.0045 0.0059 0.3891 0.0152 0.0383 0.2747 0.0264 0.0248 0.0057 0.0442 0.0163 0.0087 0.0752 0.1054
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0867 0.4219 0.0625 0.0106 0.0202 0.0376 0.0044 0.0070 0.5952 0.0286 0.0615 0.3788 0.0410 0.0324 0.0538 0.0131 0.1570 0.1658
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0853 0.3922 0.0635 0.0124 0.0147 0.0324 0.0053 0.0069 0.5319 0.0257 0.0563 0.3215 0.0331 0.0329 0.0047 0.0520 0.0186 0.0121 0.1524 0.0894
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0686 0.3257 0.0476 0.0102 0.0150 0.0278 0.0033 0.0045 0.5618 0.0197 0.0400 0.2959 0.0297 0.0271 0.0043 0.0437 0.0152 0.0107 0.1208 0.0687
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0737 0.3135 0.0479 0.0104 0.0129 0.0237 0.0040 0.0057 0.4292 0.0196 0.0383 0.2639 0.0261 0.0310 0.0056 0.0466 0.0165 0.0101 0.1229 0.0535
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0705 0.4292 0.0648 0.0153 0.0212 0.0329 0.0038 0.0054 0.5435 0.0530 0.3268 0.0380 0.0288 0.0048 0.0619 0.0192 0.0117 0.1114 0.1502
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0765 0.4132 0.0601 0.0129 0.0166 0.0278 0.0045 0.0058 0.4854 0.0183 0.0515 0.2740 0.0266 0.0295 0.0058 0.0468 0.0172 0.0102 0.1134 0.0945
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0643 0.2882 0.0405 0.0095 0.0192 0.0271 0.0043 0.0051 0.5525 0.0137 0.0312 0.2545 0.0315 0.0335 0.0037 0.0438 0.0108 0.0092 0.0937 0.1025
LOXIMVI 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0671 0.3448 0.0437 0.0096 0.0206 0.0307 0.0056 0.0070 0.4032 0.0136 0.0305 0.2667 0.0238 0.0351 0.0048 0.0433 0.0147 0.0097 0.0926 0.0945
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0943 0.2469 0.0467 0.0099 0.0164 0.0321 0.0036 0.0050 0.6410 0.0186 0.0356 0.3906 0.0408 0.0041 0.0557 0.0123 0.0092 0.1307 0.2336
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0970 0.2387 0.0397 0.0095 0.0147 0.0258 0.0043 0.0058 0.5405 0.0169 0.0382 0.3300 0.0353 0.0368 0.0047 0.0500 0.0190 0.0091 0.1261 0.0778
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1330 0.3906 0.0430 0.0137 0.0208 0.0310 0.0041 0.0049 0.6757 0.0199 0.0717 0.0445 0.0431 0.0062 0.0637 0.0156 0.0115 0.1482 0.3731
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1312 0.3861 0.0390 0.0093 0.0214 0.0254 0.0050 0.0062 0.5747 0.0183 0.0775 0.3937 0.0354 0.0368 0.0073 0.0684 0.0198 0.0125 0.1577 0.1776
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0731 0.3425 0.0503 0.0105 0.0177 0.0285 0.0038 0.0054 0.5952 0.0130 0.0352 0.3546 0.0378 0.0361 0.0041 0.0517 0.0118 0.0091 0.1195 0.2217
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0737 0.3597 0.0412 0.0101 0.0275 0.0268 0.0041 0.0058 0.5076 0.0142 0.0384 0.2778 0.0305 0.0357 0.0055 0.0459 0.0111 0.1119 0.1107
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1153 0.4274 0.0546 0.0116 0.0279 0.0047 0.0050 0.7353 0.0209 0.0375 0.4673 0.0356 0.0460 0.0066 0.0573 0.0169 0.0122 0.1522 0.3185
LOXIMVI 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1181 0.3677 0.0492 0.0111 0.0235 0.0236 0.0051 0.0068 0.5556 0.0179 0.0340 0.3704 0.0316 0.0361 0.0059 0.0609 0.0179 0.0116 0.1441 0.1580
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0781 0.3135 0.0907 0.0147 0.0152 0.0379 0.0035 0.0060 0.7092 0.0148 0.0478 0.3279 0.0301 0.0155 0.0047 0.0522 0.0170 0.0088 0.1927 0.1623
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0818 0.3390 0.0962 0.0132 0.0170 0.0385 0.0048 0.0085 0.7519 0.0203 0.0521 0.3175 0.0289 0.0199 0.0063 0.0549 0.0188 0.0088 0.1669 0.1109
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0801 0.2857 0.0760 0.0114 0.0145 0.0359 0.0039 0.0088 0.6250 0.0134 0.0390 0.3571 0.0303 0.0239 0.0049 0.0612 0.0137 0.0096 0.2252 0.1934
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0765 0.2577 0.0817 0.0106 0.0164 0.0313 0.0055 0.5917 0.0145 0.0455 0.3984 0.0348 0.0253 0.0054 0.0628 0.0205 0.0082 0.1965 0.1629
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0762 0.2941 0.0615 0.0111 0.0133 0.0361 0.0039 0.0054 0.5525 0.0146 0.0357 0.2747 0.0299 0.0198 0.0043 0.0511 0.0225 0.0079 0.1577 0.1621
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0754 0.2273 0.0604 0.0121 0.0301 0.0037 0.0082 0.6173 0.0195 0.0377 0.3068 0.0304 0.0211 0.0060 0.0627 0.0378 0.0077 0.1412 0.1030