Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.3333 5.5556 0.0354 0.0880 0.3279 0.1608 0.3571 0.0887 0.2604 0.1563 0.0905 0.1733 0.2262 0.3165 0.1144
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.3663 5.5556 0.0594 0.0881 0.3378 0.1919 0.3636 0.0939 0.2381 0.1667 0.0991 0.1733 0.2278 0.0718 0.3401 0.1215
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.3610 6.2500 0.0358 0.0963 0.3257 0.1898 0.3584 0.1071 0.2681 0.1859 0.0906 0.2288 0.2315 0.0801 0.3175 0.1019
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.3861 6.2500 0.0491 0.0907 0.3195 0.1852 0.3610 0.1068 0.2625 0.1873 0.1153 0.2519 0.2398 0.0826 0.3311 0.1203
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.3559 6.2500 0.0327 0.0869 0.1789 0.3597 0.0773 0.2646 0.1007 0.2208 0.2151 0.0712 0.3145 0.1151
C32 0.0000
uM
 24
hr
 8
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.3584 5.5556 0.0591 0.1414 0.5236 0.2008 0.2755 0.1027 0.4167 0.1610 0.1142 0.1799 0.2907 0.0764 0.5319 0.1119
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.3731 5.5556 0.0776 0.1282 0.5000 0.2217 0.2475 0.1006 0.4425 0.1639 0.1482 0.2123 0.3425 0.0995 0.5319 0.1361
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.3831 5.0000 0.0641 0.1255 0.4425 0.2058 0.2611 0.0820 0.3876 0.1575 0.1227 0.1742 0.2336 0.0705 0.4630 0.1161
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.3802 5.0000 0.0856 0.1218 0.4184 0.2132 0.2469 0.0900 0.3922 0.1506 0.1191 0.1783 0.2451 0.0924 0.4902 0.1316
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 1
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.3333 5.5556 0.0481 0.1351 0.5747 0.1802 0.2174 0.1140 0.4167 0.1341 0.1040 0.1832 0.3145 0.0793 0.5650 0.1015
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.3322 6.2500 0.0540 0.1499 0.5495 0.1953 0.1799 0.1003 0.4329 0.1319 0.1304 0.1812 0.3345 0.0839 0.5495 0.1167
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.3413 5.5556 0.0528 0.1174 0.4808 0.1718 0.2041 0.0709 0.4065 0.1211 0.1036 0.1789 0.2288 0.0589 0.5236 0.0914
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.3185 5.2632 0.0672 0.1117 0.4651 0.1972 0.1842 0.0880 0.3745 0.1325 0.1125 0.1555 0.2463 0.0674 0.5155 0.1272
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 2
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.3021 5.8824 0.0507 0.2012 0.5747 0.1880 0.2119 0.0752 0.3690 0.1289 0.0892 0.1869 0.3195 0.1049 0.5814 0.1049
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.3115 5.8824 0.0532 0.1445 0.5376 0.1866 0.1894 0.1218 0.4000 0.1300 0.1170 0.1745 0.3731 0.0713 0.5882 0.1168
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.2793 6.2500 0.0449 0.1058 0.5102 0.2092 0.1678 0.0941 0.3718 0.1239 0.0978 0.1869 0.2353 0.0514 0.5236 0.0403