Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3968 6.2500 0.0575 0.1238 0.3817 0.1848 0.4167 0.1502 0.4049 0.1577 0.0988 0.1927 0.3215 0.0694 0.3831 0.1300
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3861 7.1429 0.0553 0.1399 0.3650 0.2160 0.3247 0.1332 0.3906 0.1647 0.1724 0.4016 0.3922 0.1271
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3968 6.2500 0.0474 0.1057 0.2916 0.1764 0.3650 0.1001 0.3831 0.1202 0.0983 0.1908 0.2457 0.1129 0.3106 0.1146
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 5.8824 0.0663 0.0997 0.3401 0.2119 0.3425 0.1116 0.3677 0.1429 0.1068 0.1605 0.4082 0.0680 0.3367 0.1333
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3861 6.6667 0.1247 0.3367 0.2004 0.4000 0.1076 0.4310 0.1527 0.1214 0.2421 0.2571 0.0664 0.3311 0.1221
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3906 7.6923 0.0596 0.1191 0.3145 0.2203 0.3677 0.1387 0.4184 0.1577 0.1284 0.2049 0.2625 0.0951 0.3322 0.1462
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3861 7.1429 0.0440 0.1014 0.3012 0.1876 0.3610 0.0900 0.3937 0.1423 0.1103 0.2370 0.1992 0.0543 0.3257 0.1092
C32 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.3759 5.8824 0.0766 0.1130 0.2994 0.2252 0.3472 0.1016 0.3650 0.1531 0.1191 0.1912 0.2433 0.1136 0.3322 0.0822
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.3472 6.6667 0.0436 0.1639 0.4115 0.2058 0.3953 0.3597 0.1567 0.0996 0.1842 0.3012 0.0925 0.1161
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.3509 7.1429 0.0681 0.1730 0.3891 0.2101 0.3413 0.1429 0.3891 0.1751 0.1007 0.1754 0.4525 0.0997 0.3546 0.1091
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.3115 6.6667 0.1314 0.1056 0.3846 0.1996 0.3311 0.1082 0.3623 0.1445 0.1026 0.1919 0.3165 0.0827 0.3521 0.1206
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.3953 6.2500 0.0377 0.1227 0.3731 0.2137 0.3390 0.1259 0.3906 0.1795 0.1226 0.1621 0.4405 0.0825 0.3817 0.1423
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.2994 7.1429 0.0419 0.1143 0.3571 0.2141 0.3953 0.1248 0.3906 0.1511 0.1006 0.2770 0.2941 0.0770 0.3650 0.0943
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.3597 7.1429 0.0435 0.1155 0.3356 0.2114 0.3922 0.1220 0.4082 0.1859 0.1321 0.2075 0.3279 0.0893 0.3745 0.1053
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.2907 7.1429 0.0364 0.0943 0.3135 0.1972 0.3413 0.1034 0.3571 0.1351 0.1006 0.2639 0.2045 0.3759 0.0947
C32 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.4065 6.2500 0.0988 0.1085 0.3012 0.3802 0.1151 0.3663 0.1835 0.1337 0.1934 0.3003 0.0826 0.3817 0.1393
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.3436 6.2500 0.0507 0.1223 0.3718 0.1972 0.3663 0.3068 0.1718 0.0937 0.1779 0.2941 0.0854 0.3497 0.1248
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.4202 6.6667 0.0556 0.1126 0.3718 0.1855 0.2801 0.1055 0.3175 0.1786 0.1285 0.2160 0.2625 0.0895 0.3663 0.1368
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.3356 5.8824 0.0360 0.0994 0.3401 0.1745 0.2857 0.1136 0.2577 0.1656 0.0844 0.1869 0.1919 0.0687 0.3356 0.0676
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.4049 5.8824 0.0636 0.1107 0.3460 0.2037 0.3571 0.1023 0.2488 0.1842 0.1109 0.1637 0.2688 0.0805 0.3509 0.1323
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.3774 6.6667 0.0351 0.1033 0.3236 0.1779 0.3861 0.1076 0.2899 0.1786 0.1005 0.2336 0.2294 0.0818 0.3300 0.1012
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.4255 7.1429 0.0564 0.1192 0.3268 0.2028 0.3906 0.1088 0.2941 0.1873 0.1253 0.2488 0.2674 0.0861 0.3378 0.1248
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.3472 6.2500 0.0315 0.0965 0.2817 0.1634 0.3509 0.2681 0.1799 0.1005 0.2004 0.1650 0.0676 0.3195 0.1122
C32 0.0000
uM
 24
hr
 4
InnoScan/Mapix
C32 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.4202 6.2500 0.0655 0.1198 0.4310 0.2262 0.4785 0.1412 0.4082 0.1852 0.1080 0.1770 0.4032 0.0923 0.3690 0.1299