Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3279 7.6923 0.0541 0.1041 0.2740 0.2183 0.4292 0.0944 0.3663 0.1464 0.1206 0.2717 0.1764 0.0739 0.6250 0.1157
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.4566 7.1429 0.0898 0.1106 0.2747 0.2551 0.5263 0.1209 0.3984 0.1859 0.1524 0.2020 0.2890 0.0808 0.6211 0.1370
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4049 7.6923 0.0675 0.1230 0.3236 0.2278 0.5102 0.0835 0.3623 0.1988 0.1023 0.2817 0.4000 0.0927 0.5236 0.1306
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4367 8.3333 0.0737 0.1125 0.2899 0.2193 0.4854 0.1391 0.3788 0.2062 0.1529 0.3311 0.4082 0.1014 0.5348 0.1565
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.3817 7.1429 0.0671 0.1182 0.2294 0.4902 0.0998 0.3086 0.1859 0.0928 0.3077 0.1988 0.0743 0.5405 0.1255
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4310 7.1429 0.0744 0.2732 0.2342 0.5051 0.1157 0.2933 0.1980 0.1297 0.2755 0.3068 0.0899 0.5556 0.1748
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.3846 7.6923 0.0578 0.1271 0.3448 0.2299 0.5208 0.1387 0.3677 0.2146 0.1143 0.2725 0.2817 0.0958 0.5682 0.1131
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4329 8.3333 0.0735 0.1276 0.3472 0.2416 0.5000 0.1379 0.3788 0.1464 0.2941 0.4405 0.1045 0.5650 0.1471
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4049 7.1429 0.0478 0.1344 0.2865 0.2347 0.5495 0.1044 0.3425 0.1961 0.1201 0.2639 0.2381 0.0875 0.6250 0.1410
C32 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.4405 6.2500 0.1221 0.2967 0.1185 0.3356 0.2092 0.1471 0.2288 0.3356 0.0895 0.6452 0.1721
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 7.6923 0.0994 0.1139 0.3610 0.2809 0.5917 0.1565 0.4951 0.2320 0.1280 0.3077 0.3861 0.0939 0.6494 0.1333
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4673 8.3333 0.0870 0.1410 0.3290 0.2639 0.5682 0.5263 0.2439 0.3731 0.3953 0.1042 0.6369 0.1818
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4525 7.6923 0.0844 0.1235 0.3279 0.2584 0.6250 0.1266 0.4464 0.1953 0.1256 0.3086 0.3704 0.0826 0.5917 0.1435
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4673 7.1429 0.1070 0.1370 0.3040 0.2632 0.5650 0.1319 0.4348 0.1812 0.1208 0.3401 0.2849 0.0980 0.6211
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4651 8.3333 0.0699 0.1423 0.3663 0.2320 0.1466 0.4762 0.2252 0.1229 0.2825 0.3205 0.0832 0.6135 0.1490
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4348 6.6667 0.0899 0.1361 0.3413 0.2725 0.5780 0.1361 0.4831 0.2004 0.1339 0.3115 0.3677 0.1022 0.5682 0.1441
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4505 7.1429 0.0802 0.1086 0.2841 0.2463 0.6061 0.1075 0.4739 0.1712 0.1105 0.2786 0.2262 0.0637 0.5465 0.1733
C32 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.4115 6.6667 0.1013 0.1294 0.2747 0.2681 0.6173 0.1136 0.4386 0.1580 0.1377 0.2326 0.2392 0.0933 0.5618 0.1704
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.3922 7.6923 0.0802 0.1088 0.3448 0.2262 0.5714 0.1524 0.4673 0.1736 0.1198 0.3226 0.4444 0.0998 0.5714 0.1597
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.4032 10.0000 0.0609 0.1414 0.3096 0.2519 0.4546 0.1572 0.3472 0.1767 0.1539 0.3003 0.3247 0.5525 0.1653
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.4082 7.6923 0.0655 0.1202 0.2841 0.2217 0.5465 0.1361 0.4329 0.1565 0.0976 0.3279 0.4348 0.0708 0.5587 0.1229
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.3623 7.1429 0.0822 0.2959 0.2439 0.4329 0.1353 0.4115 0.1789 0.1205 0.2801 0.3534 0.0773 0.5814 0.1464
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.4310 7.6923 0.0693 0.1307 0.3817 0.1852 0.5556 0.1497 0.5025 0.1845 0.1181 0.2817 0.4651 0.0777 0.5556 0.1214
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.3677 9.0909 0.0743 0.1277 0.3704 0.2513 0.4444 0.1471 0.5025 0.1905 0.1447 0.2584 0.3623 0.0838 0.5587 0.1333
C32 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.3906 8.3333 0.0695 0.1035 0.3279 0.2183 0.5181 0.1138 0.5000 0.1570 0.1146 0.2597 0.3247 0.0670 0.5952 0.1115