Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.5076 7.1429 0.0663 0.0981 0.2976 0.2203 0.4975 0.1527 0.3279 0.2247 0.1653 0.4525 0.3040 0.0911 0.5155 0.1323
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4149 4.3478 0.0361 0.0811 0.1980 0.2075 0.5348 0.0794 0.2985 0.1280 0.1280 0.4149 0.1462 0.0686 0.3891 0.1209
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4630 6.2500 0.0732 0.0883 0.2016 0.2451 0.5650 0.0945 0.2857 0.1321 0.1550 0.2268 0.1748 0.0698 0.4202 0.1650
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4464 8.3333 0.1008 0.1263 0.3185 0.2506 0.5917 0.1477 0.5076 0.2398 0.3257 0.4274 0.1068 0.5525 0.1318
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4785 8.3333 0.0864 0.1181 0.2899 0.2695 0.5952 0.1590 0.5208 0.2193 0.1692 0.3460 0.4444 0.1258 0.5291 0.1669
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4673 7.1429 0.0695 0.1109 0.2793 0.2513 0.5556 0.1171 0.4348 0.1898 0.1410 0.3290 0.2421 0.0742 0.4902 0.1374
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4219 7.1429 0.0998 0.1279 0.2703 0.2392 0.5435 0.1277 0.4237 0.1692 0.1475 0.3125 0.4016 0.0943 0.5076 0.1647
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4739 7.6923 0.0797 0.1201 0.3205 0.2725 0.5988 0.1160 0.4525 0.2169 0.1195 0.2732 0.2494 0.0701 0.6098 0.1508
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4673 6.2500 0.0970 0.1221 0.2976 0.2732 0.5714 0.1116 0.4566 0.1873 0.1302 0.2604 0.3145 0.0994 0.5952 0.1698
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4566 7.1429 0.0794 0.2577 0.2451 0.6250 0.0838 0.4739 0.1698 0.1328 0.2519 0.1730 0.0687 0.5348 0.1629
C32 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.4202 6.2500 0.1047 0.1136 0.2558 0.2463 0.5587 0.1064 0.4237 0.1495 0.1292 0.2475 0.2088 0.0913 0.5650
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 7.6923 0.0638 0.1135 0.3106 0.1883 0.5495 0.1497 0.4425 0.1761 0.0951 0.3472 0.3546 0.0716 0.5348 0.1139
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.4032 8.3333 0.0698 0.1297 0.2874 0.2288 0.4546 0.1534 0.4546 0.1869 0.1403 0.3012 0.3300 0.0840 0.5556 0.1316
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.4115 7.6923 0.0602 0.1242 0.2597 0.2203 0.5435 0.0899 0.4032 0.1548 0.1242 0.3546 0.3135 0.0618 0.4717 0.1224
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.3876 7.1429 0.0765 0.0938 0.2653 0.2469 0.5376 0.1325 0.4132 0.1621 0.1376 0.2646 0.3460 0.0700 0.5319 0.1550
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.4329 8.3333 0.0664 0.1144 0.3448 0.2105 0.5714 0.1511 0.5291 0.1767 0.1163 0.2967 0.3425 0.0848 0.6667 0.1337
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.4065 9.0909 0.0880 0.1232 0.3345 0.2725 0.4902 0.1605 0.4951 0.1927 0.1416 0.2283 0.4016 0.0883 0.6897 0.1499
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.3922 8.3333 0.0686 0.0959 0.3215 0.1832 0.5000 0.1159 0.5051 0.1592 0.1236 0.2874 0.2778 0.0260 0.5155 0.1383
C32 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.4065 6.6667 0.0975 0.1341 0.3021 0.2625 0.5025 0.1433 0.4310 0.1953 0.1468 0.3831 0.0211 0.6135 0.1468
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3448 8.3333 0.0575 0.1518 0.3345 0.2500 0.5236 0.1647 0.4132 0.1754 0.1070 0.2907 0.4926 0.1068 0.6061 0.1107
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3876 9.0909 0.0688 0.3401 0.2268 0.4878 0.1859 0.4348 0.2123 0.1553 0.2959 0.4695 0.0243 0.6024 0.1300
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3413 8.3333 0.0667 0.1140 0.3115 0.2217 0.5682 0.1416 0.3953 0.1724 0.1212 0.2457 0.3559 0.0534 0.6369 0.1033
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.4329 7.6923 0.0966 0.1201 0.3096 0.2427 0.5236 0.1475 0.4049 0.2392 0.1429 0.2347 0.3690 0.0974 0.6369 0.1421
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3185 8.3333 0.0509 0.1092 0.3509 0.2283 0.5556 0.1529 0.4149 0.1828 0.0998 0.2916 0.3401 0.0982 0.6536 0.1206
C32 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.3891 8.3333 0.0779 0.1223 0.3390 0.2421 0.4831 0.1445 0.2169 0.1502 0.2304 0.3759 0.1013 0.6623 0.1306