Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0819 0.4651 0.0110 0.0120 0.0135 0.1600 0.0258 0.1482 0.0514 0.0291 0.2519 0.0289 0.0517 0.0078 0.0672 0.0184 0.1199 0.0298
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0951 0.4951 0.0107 0.0148 0.0170 0.1350 0.0267 0.1271 0.0490 0.0296 0.2500 0.0278 0.0583 0.0073 0.0612 0.0170 0.1385 0.0253
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0821 0.4310 0.0112 0.0119 0.0111 0.1117 0.0235 0.1418 0.0405 0.0264 0.1786 0.0236 0.0511 0.0074 0.0546 0.0158 0.1094 0.0339
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0892 0.4219 0.0111 0.0133 0.0162 0.1223 0.0245 0.1433 0.0392 0.0300 0.1942 0.0212 0.0497 0.0076 0.0513 0.0154 0.1151 0.0311
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0956 0.5291 0.0123 0.0123 0.0128 0.1616 0.0273 0.1605 0.0471 0.0323 0.2674 0.0345 0.0663 0.0076 0.0797 0.0219 0.1439 0.0257
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.1047 0.5208 0.0116 0.0142 0.0179 0.1565 0.0272 0.1565 0.0490 0.0305 0.2907 0.0292 0.0717 0.0090 0.0765 0.0221 0.1529 0.0247
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0889 0.4386 0.0103 0.0132 0.0106 0.1068 0.0210 0.1300 0.0328 0.0252 0.1795 0.0225 0.0591 0.0070 0.0438 0.0152 0.1082 0.0214
WM1552C 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.0997 0.4255 0.0125 0.0134 0.0116 0.1292 0.0228 0.1490 0.0321 0.0305 0.2012 0.0203 0.0564 0.0087 0.0439 0.0150 0.1067 0.0230
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0850 0.3861 0.0096 0.0137 0.0136 0.1075 0.0236 0.1294 0.0391 0.0290 0.1475 0.0229 0.0498 0.0067 0.0403 0.0118 0.0957
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0962 0.4016 0.0116 0.0133 0.0134 0.1020 0.0189 0.1223 0.0309 0.0280 0.1447 0.0203 0.0551 0.0079 0.0413 0.0132 0.0982 0.0286
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0855 0.4000 0.0096 0.0122 0.0102 0.0903 0.0196 0.1230 0.0285 0.0243 0.1227 0.0193 0.0469 0.0070 0.0358 0.0122 0.0806 0.0246
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0962 0.3509 0.0102 0.0127 0.0137 0.0951 0.0183 0.1221 0.0268 0.0257 0.1224 0.0170 0.0499 0.0075 0.0333 0.0127 0.0843 0.0222
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0957 0.5747 0.0118 0.0123 0.0152 0.1883 0.0323 0.1976 0.0541 0.0367 0.3021 0.0392 0.0693 0.0081 0.1001 0.0224 0.1232 0.0283
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.1045 0.5882 0.0128 0.0117 0.0163 0.1880 0.0265 0.1835 0.0534 0.0353 0.3096 0.0332 0.0732 0.0082 0.0897 0.0257 0.1361 0.0259
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0936 0.4673 0.0100 0.0119 0.0111 0.1152 0.0238 0.1401 0.0387 0.0259 0.1890 0.0209 0.0555 0.0080 0.0544 0.0142 0.1282 0.0204
WM1552C 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.1011 0.4444 0.0105 0.0129 0.0116 0.1364 0.0223 0.1698 0.0403 0.0297 0.1949 0.0225 0.0484 0.0078 0.0458 0.0157 0.1134 0.0222
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0773 0.4673 0.0105 0.0108 0.1332 0.0263 0.0472 0.0252 0.1934 0.0358 0.0265 0.0066 0.0602 0.0169 0.1144 0.0462
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0855 0.5236 0.0116 0.0131 0.0136 0.1189 0.0245 0.0470 0.0292 0.1938 0.0407 0.0348 0.0078 0.0600 0.0207 0.1214 0.0308
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0843 0.4310 0.0110 0.0117 0.0141 0.1074 0.0247 0.1468 0.0375 0.0255 0.1466 0.0282 0.0283 0.0076 0.0527 0.0161 0.0876 0.0547
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0961 0.4484 0.0134 0.0122 0.0148 0.1201 0.0281 0.1504 0.0366 0.0300 0.1707 0.0325 0.0340 0.0096 0.0455 0.0169 0.0929 0.0330
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0783 0.5263 0.0113 0.0116 0.0164 0.1359 0.0247 0.1488 0.0374 0.0304 0.1852 0.0355 0.0238 0.0069 0.0650 0.0183 0.1189 0.0457
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0872 0.5882 0.0133 0.0125 0.0134 0.1350 0.0302 0.1520 0.0362 0.0350 0.1946 0.0401 0.0296 0.0078 0.0619 0.0234 0.1140 0.0371
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0775 0.4587 0.0113 0.0115 0.0105 0.0992 0.0192 0.1350 0.0308 0.0284 0.1425 0.0241 0.0241 0.0070 0.0462 0.0163 0.0724 0.0458
WM1552C 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0820 0.5236 0.0143 0.0122 0.0134 0.1143 0.0208 0.1522 0.0221 0.0333 0.1475 0.0249 0.0262 0.0087 0.0380 0.0162 0.0665 0.0245
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0797 0.5000 0.0108 0.0133 0.1397 0.0264 0.1244 0.0492 0.0305 0.2020 0.0377 0.0285 0.0072 0.0766 0.0182 0.1119 0.0374