Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0955 0.6623 0.0114 0.0109 0.0175 0.1678 0.0341 0.2364 0.0309 0.0348 0.3300 0.0505 0.0663 0.0092 0.0726 0.0167 0.0916 0.0279
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0925 0.7353 0.0145 0.0107 0.0145 0.2169 0.0389 0.2618 0.0368 0.0378 0.4184 0.0649 0.0740 0.0100 0.1179 0.0243 0.0990 0.0302
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.1019 0.7519 0.0143 0.0213 0.2247 0.0418 0.2359 0.0362 0.0396 0.4348 0.0592 0.0783 0.0107 0.0972 0.0206 0.1100 0.0274
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0881 0.6579 0.0105 0.0099 0.0128 0.1650 0.0298 0.2294 0.0284 0.0312 0.3300 0.0539 0.0683 0.0087 0.0711 0.0162 0.0841 0.0255
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0935 0.6944 0.0129 0.0105 0.0173 0.2000 0.0323 0.2717 0.0302 0.0385 0.3546 0.0517 0.0582 0.0108 0.0662 0.0205 0.0855 0.0292
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0843 0.6098 0.0128 0.0106 0.0162 0.1383 0.0298 0.2513 0.0325 0.0322 0.2506 0.0467 0.0689 0.0089 0.0548 0.0110 0.0616
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0935 0.5988 0.0123 0.0115 0.0147 0.1247 0.0281 0.2128 0.0311 0.0310 0.2551 0.0504 0.0671 0.0091 0.0416 0.0128 0.0766 0.0256
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0812 0.6536 0.0111 0.0099 0.0096 0.1456 0.0289 0.2273 0.0298 0.0322 0.2770 0.0598 0.0623 0.0087 0.0644 0.0150 0.0909 0.0256
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0920 0.5882 0.0116 0.0109 0.0136 0.1414 0.0273 0.2310 0.0312 0.0322 0.2762 0.0583 0.0564 0.0092 0.0600 0.0144 0.0855 0.0249
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0907 0.7092 0.0136 0.0108 0.0182 0.2000 0.2398 0.0352 0.0365 0.3718 0.0717 0.0714 0.0099 0.0977 0.0170 0.0904 0.0284
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0950 0.7092 0.0139 0.0115 0.0184 0.1898 0.0282 0.2359 0.0335 0.0378 0.3636 0.0624 0.0770 0.0103 0.0832 0.0224 0.0978 0.0241
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0844 0.6944 0.0120 0.0104 0.0104 0.1454 0.0273 0.2381 0.0301 0.0339 0.2809 0.0479 0.0654 0.0104 0.0583 0.0135 0.0832 0.0265
WM1552C 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0903 0.7042 0.0124 0.0104 0.0112 0.1739 0.0253 0.0317 0.0377 0.3040 0.0552 0.0527 0.0095 0.0535 0.0176 0.0768 0.0258
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0838 0.7692 0.0112 0.0123 0.0146 0.1751 0.0364 0.2203 0.0323 0.0334 0.3484 0.0456 0.0301 0.0069 0.0727 0.1319 0.0292
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0900 0.8547 0.0123 0.0150 0.0162 0.1724 0.0319 0.2801 0.0289 0.0328 0.3534 0.0503 0.0382 0.0086 0.0679 0.0198 0.1407 0.0232
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0864 0.7092 0.0115 0.0128 0.0177 0.1410 0.0351 0.2632 0.0290 0.0303 0.2890 0.0377 0.0324 0.0071 0.0679 0.0199 0.1290 0.0378
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0948 0.7813 0.0136 0.0145 0.0226 0.1527 0.0365 0.2793 0.0306 0.0339 0.3049 0.0300 0.0384 0.0089 0.0559 0.0172 0.1314 0.0236
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0824 0.6993 0.0132 0.0157 0.0263 0.2096 0.0370 0.2237 0.0422 0.0325 0.3906 0.0487 0.0292 0.0076 0.1028 0.0196 0.1409 0.0417
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0948 0.7874 0.0144 0.0145 0.0214 0.2058 0.2427 0.0411 0.0380 0.4237 0.0506 0.0363 0.0098 0.0989 0.0215 0.1454 0.0269
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0873 0.6494 0.0110 0.0136 0.0187 0.1555 0.0279 0.2141 0.0356 0.0314 0.3268 0.0286 0.0272 0.0072 0.0827 0.0207 0.1201 0.0332
WM1552C 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0902 0.7092 0.0163 0.0136 0.0248 0.1890 0.0310 0.2809 0.0370 0.0395 0.3215 0.0331 0.0341 0.0090 0.0633 0.0195 0.1035 0.0228
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0804 0.7813 0.0123 0.0129 0.0166 0.1916 0.0349 0.2416 0.0318 0.0316 0.3509 0.0443 0.0280 0.0077 0.0807 0.0174 0.1362 0.0294
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0870 0.8130 0.0115 0.0136 0.0178 0.1631 0.0376 0.2179 0.0295 0.0328 0.3663 0.0408 0.0345 0.0088 0.0785 0.0200 0.1524 0.0225
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0798 0.6579 0.0106 0.0128 0.0140 0.1348 0.0285 0.1984 0.0246 0.0275 0.2387 0.0295 0.0282 0.0069 0.0529 0.0142 0.1025 0.0362
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0810 0.7042 0.0119 0.0142 0.0195 0.1416 0.0315 0.2183 0.0235 0.0317 0.2506 0.0262 0.0299 0.0079 0.0441 0.0122 0.1032 0.0282