Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 8.3333 0.0595 0.4000 0.2564 0.7092 0.1901 0.5495 0.2398 0.1520 0.4016 0.4132 0.0929 0.5435 0.1482
C32 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.5263 7.6923 0.0736 0.1304 0.3597 0.2833 0.6536 0.1786 0.5405 0.2062 0.1890 0.4329 0.4425 0.1058 0.5236 0.1563
C32 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.5376 8.3333 0.0688 0.1239 0.3484 0.2451 0.6623 0.1658 0.5848 0.2146 0.1650 0.4082 0.3390 0.0633 0.4808 0.1253
C32 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.5291 7.6923 0.0993 0.1284 0.3175 0.2941 0.6579 0.1742 0.5348 0.1976 0.1590 0.4032 0.3226 0.1140 0.5051 0.1623
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4348 8.3333 0.0603 0.1205 0.4630 0.2320 0.6579 0.1776 0.5181 0.1792 0.3425 0.4016 0.5682 0.1097
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4274 10.0000 0.0583 0.1403 0.4184 0.2525 0.5435 0.1880 0.5076 0.1852 0.1667 0.3484 0.3690 0.0867 0.5495 0.1431
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4505 8.3333 0.0599 0.1017 0.4237 0.2571 0.5952 0.1656 0.5465 0.1761 0.1605 0.3650 0.3984 0.0840 0.5102 0.1344
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4525 7.6923 0.1174 0.4065 0.2882 0.5155 0.1898 0.4975 0.1953 0.1758 0.3030 0.4464 0.0981 0.5376 0.1531
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4546 7.6923 0.0504 0.1253 0.3788 0.2273 0.1845 0.5405 0.1869 0.1355 0.4329 0.3788 0.0715 0.5208 0.1269
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4367 9.0909 0.0644 0.1285 0.3623 0.2674 0.5128 0.1783 0.5181 0.1919 0.1650 0.3484 0.0878 0.5291 0.1570
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4566 0.0622 0.1110 0.3390 0.2359 0.5988 0.1497 0.5155 0.1645 0.1482 0.4255 0.2994 0.1019 0.5181 0.1383
C32 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.4505 6.6667 0.0869 0.1153 0.2924 0.2874 0.5814 0.1686 0.4831 0.1876 0.1792 0.3279 0.3559 0.0938 0.5348
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.3953 8.3333 0.0591 0.1372 0.3922 0.2653 0.6135 0.1988 0.4274 0.1862 0.1300 0.3610 0.2924 0.1143 0.5988 0.1258
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.4167 9.0909 0.0524 0.3876 0.2618 0.4367 0.1992 0.4762 0.2110 0.1754 0.4149 0.5319 0.1192 0.5714 0.1346
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.3472 7.6923 0.0454 0.0959 0.3356 0.2488 0.3817 0.1414 0.3802 0.1212 0.1447 0.3922 0.2427 0.0729 0.4464 0.1107
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.4739 7.1429 0.0743 0.0921 0.3195 0.2577 0.4132 0.1468 0.3968 0.1919 0.1520 0.2976 0.2865 0.0568 0.4425 0.1284
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.3663 7.1429 0.0496 0.1068 0.3460 0.2519 0.5525 0.1709 0.4115 0.1901 0.1274 0.3968 0.4115 0.1063 0.6369 0.0888
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.4630 8.3333 0.0766 0.1120 0.3521 0.2545 0.5587 0.1745 0.4386 0.2193 0.1715 0.3571 0.4032 0.0805 0.6024 0.1359
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.3333 8.3333 0.0581 0.1042 0.3125 0.2410 0.4831 0.1416 0.3774 0.1546 0.1335 0.4000 0.2445 0.0765 0.5556 0.1548
C32 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.5102 7.1429 0.0831 0.1037 0.3086 0.5076 0.1590 0.4016 0.1776 0.1692 0.2916 0.2924 0.0818 0.5435 0.1477
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4329 4.3478 0.0798 0.1083 0.3718 0.2538 0.5556 0.1464 0.3521 0.2101 0.1299 0.2916 0.3390 0.0940 0.4975 0.1214
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4386 7.6923 0.0595 0.0937 0.3460 0.2137 0.4444 0.1439 0.3718 0.2179 0.1546 0.3106 0.3125 0.1010 0.4831 0.1196
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4444 4.5455 0.0541 0.1029 0.3484 0.2404 0.6061 0.1348 0.3472 0.1992 0.1294 0.2967 0.2740 0.0778 0.5348 0.1129
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4831 6.6667 0.0820 0.1114 0.3759 0.2538 0.5780 0.1414 0.3390 0.2273 0.1482 0.3195 0.3040 0.0923 0.5319 0.1477
C32 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.4505 7.1429 0.0629 0.1038 0.2959 0.2336 0.5465 0.1468 0.3236 0.2105 0.1372 0.4049 0.2857 0.0907 0.4975 0.1087