Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0883 0.5682 0.0146 0.0095 0.0283 0.2033 0.0333 0.2778 0.0456 0.0362 0.4098 0.0364 0.0308 0.0092 0.0753 0.0211 0.0524 0.0235
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0839 0.6803 0.0139 0.0091 0.0220 0.2364 0.0386 0.2625 0.0391 0.0355 0.4608 0.0457 0.0381 0.0088 0.1155 0.0236 0.0649 0.0306
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0885 0.7194 0.0123 0.0109 0.0294 0.2101 0.0457 0.2591 0.0387 0.0337 0.4695 0.0424 0.0385 0.0084 0.1170 0.0244 0.0755 0.0262
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0855 0.7299 0.0115 0.0085 0.0208 0.1972 0.0393 0.2237 0.0381 0.0349 0.4098 0.0460 0.0394 0.0090 0.1055 0.0234 0.0563 0.0306
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0932 0.7353 0.0150 0.0101 0.0297 0.2222 0.0424 0.2532 0.0414 0.0387 0.4274 0.0355 0.0406 0.0104 0.1250 0.0598 0.0280
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0796 0.5291 0.0126 0.0094 0.0275 0.2237 0.0384 0.2387 0.0540 0.0353 0.4546 0.0504 0.0280 0.0084 0.1348 0.0243 0.0567 0.0267
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0879 0.5405 0.0139 0.0108 0.0325 0.2188 0.0390 0.2370 0.0523 0.0375 0.4902 0.0451 0.0310 0.0104 0.1294 0.0252 0.0639 0.0250
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0794 0.5236 0.0101 0.0096 0.0238 0.1695 0.0307 0.1905 0.0400 0.3745 0.0345 0.0265 0.0080 0.0788 0.0484 0.0229
WM1552C 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0898 0.5319 0.0137 0.0101 0.0261 0.2004 0.0327 0.2571 0.0442 0.0358 0.4016 0.0328 0.0290 0.0104 0.0912 0.0235 0.0491 0.0270
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0810 0.6579 0.0310 0.2347 0.0416 0.2500 0.0352 0.0387 0.4608 0.0510 0.0327 0.0082 0.1221 0.0216 0.0599
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0870 0.6623 0.0144 0.0101 0.0201 0.2183 0.0388 0.2398 0.0409 0.0356 0.4310 0.0391 0.0362 0.0091 0.0947 0.0199 0.0725 0.0294
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0849 0.6757 0.0142 0.0096 0.0213 0.2024 0.0403 0.2347 0.0352 0.0344 0.4184 0.0424 0.0347 0.0086 0.1151 0.0212 0.0674 0.0290
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0929 0.6536 0.0140 0.0108 0.0217 0.2278 0.0322 0.2494 0.0380 0.0388 0.4032 0.0365 0.0364 0.0099 0.1076 0.0211 0.0691 0.0286
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0858 0.5319 0.0106 0.0344 0.2513 0.0404 0.2364 0.0598 0.0436 0.4926 0.0600 0.0319 0.0112 0.2028 0.0277 0.0661 0.0297
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0941 0.5263 0.0144 0.0118 0.0338 0.2538 0.0300 0.2427 0.0608 0.0462 0.4926 0.0495 0.0343 0.0108 0.1536 0.0283 0.0750 0.0288
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0802 0.4854 0.0141 0.0104 0.0281 0.1869 0.0304 0.0417 0.0340 0.3937 0.0291 0.0292 0.0084 0.0734 0.0128 0.0486
WM1552C 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0894 0.5128 0.0141 0.0101 0.0267 0.2179 0.0307 0.2618 0.0439 0.0422 0.3906 0.0325 0.0237 0.0106 0.0648 0.0162 0.0526 0.0255
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0792 0.5714 0.0130 0.0205 0.2151 0.0357 0.2320 0.0397 0.0295 0.3953 0.0385 0.0294 0.0079 0.0807 0.0134 0.0510 0.0475
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0821 0.5882 0.0130 0.0104 0.0209 0.1859 0.0288 0.2096 0.0354 0.0304 0.3906 0.0366 0.0369 0.0086 0.0668 0.0234 0.0548 0.0308
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0878 0.6329 0.0119 0.0103 0.0241 0.1745 0.0309 0.2416 0.0376 0.0300 0.3597 0.0349 0.0325 0.0083 0.0759 0.0196 0.0435 0.0501
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0912 0.6536 0.0136 0.0108 0.0268 0.1898 0.0337 0.2551 0.0350 0.0336 0.3802 0.0293 0.0380 0.0089 0.0701 0.0164 0.0504 0.0335
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0838 0.4854 0.0113 0.0101 0.0241 0.2188 0.0337 0.2105 0.0568 0.0284 0.4348 0.0504 0.0270 0.0091 0.1073 0.0204 0.0704 0.0297
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0890 0.5495 0.0134 0.0111 0.0261 0.2037 0.0392 0.2320 0.0540 0.0310 0.4651 0.0416 0.0310 0.0094 0.1055 0.0228 0.0776 0.0230
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0866 0.4854 0.0111 0.0100 0.0219 0.1751 0.0258 0.1919 0.0435 0.0268 0.3311 0.0332 0.0273 0.0079 0.0840 0.0196 0.0482 0.0363
WM1552C 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0858 0.5208 0.0139 0.0104 0.0335 0.1901 0.0289 0.2160 0.0446 0.0352 0.3690 0.0296 0.0316 0.0091 0.0623 0.0163 0.0487 0.0289