Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0274 0.1637 0.0273 0.0122 0.0038 0.0047 0.0037 0.0043 0.1387 0.0190 0.0355 0.0367 0.0102 0.0065 0.0039 0.0055 0.0050 0.0069 0.0878 0.0401
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0363 0.2020 0.0389 0.0143 0.0053 0.0061 0.0036 0.0046 0.0056 0.0832 0.0355 0.0306 0.0602 0.0129 0.0045 0.0058 0.0092 0.0921
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0300 0.1473 0.0408 0.0175 0.0036 0.0048 0.0030 0.0041 0.0042 0.1170 0.0272 0.0411 0.0372 0.0098 0.0058 0.0040 0.0061 0.0060 0.0083 0.0811 0.0486
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0345 0.2037 0.0473 0.0175 0.0038 0.0064 0.0032 0.0038 0.0046 0.0931 0.0300 0.0342 0.0529 0.0119 0.0054 0.0034 0.0068 0.0088 0.0089 0.0858 0.0702
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0226 0.1715 0.0294 0.0130 0.0041 0.0046 0.0028 0.0037 0.0042 0.0820 0.0206 0.0172 0.0360 0.0100 0.0056 0.0039 0.0051 0.0067 0.0072 0.0958 0.0375
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0306 0.1942 0.0302 0.0152 0.0044 0.0053 0.0028 0.0041 0.0040 0.0938 0.0240 0.0164 0.0476 0.0107 0.0059 0.0035 0.0054 0.0076 0.0078 0.1014 0.0552
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0462 0.2294 0.0359 0.0182 0.0046 0.0062 0.0034 0.0049 0.0054 0.0240 0.0236 0.0143 0.0524 0.0134 0.0096 0.0040 0.0066 0.0082 0.0105 0.1285 0.0669
WM1552C 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0560 0.2611 0.0294 0.0201 0.0049 0.0071 0.0036 0.0047 0.0059 0.0257 0.0296 0.0170 0.0749 0.0149 0.0110 0.0065 0.0113 0.0124 0.1410 0.0846
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0879 0.7353 0.0164 0.0095 0.0193 0.2262 0.0370 0.2294 0.0406 0.0312 0.4310 0.0478 0.0357 0.0086 0.0907 0.0176 0.0631 0.0280
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0934 0.7752 0.0129 0.0108 0.0215 0.2088 0.0426 0.2660 0.0367 0.0322 0.4348 0.0502 0.0435 0.0098 0.0981 0.0215 0.0760 0.0236
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0895 0.7246 0.0121 0.0097 0.0181 0.1773 0.0345 0.2494 0.0331 0.0292 0.3861 0.0372 0.0394 0.0079 0.0815 0.0240 0.0618 0.0293
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0953 0.7874 0.0142 0.0103 0.0279 0.1923 0.0389 0.2882 0.0365 0.0341 0.4167 0.0276 0.0411 0.0093 0.0773 0.0165 0.0647 0.0258
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0890 0.6579 0.0127 0.0101 0.0333 0.2457 0.0400 0.2681 0.0530 0.0342 0.4695 0.0509 0.0334 0.0099 0.1295 0.0267 0.0675
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0972 0.7092 0.0139 0.0104 0.0270 0.2262 0.0464 0.2874 0.0568 0.0380 0.5076 0.0544 0.0379 0.0091 0.1565 0.0276 0.0712 0.0280
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0925 0.6329 0.0115 0.0096 0.0234 0.1808 0.0337 0.2203 0.0440 0.0307 0.4049 0.0363 0.0339 0.0083 0.0991 0.0265 0.0478 0.0284
WM1552C 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0963 0.6993 0.0175 0.0092 0.0334 0.2257 0.0366 0.2933 0.0451 0.0373 0.4329 0.0353 0.0377 0.0106 0.0889 0.0221 0.0467 0.0238
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0835 0.6944 0.0137 0.0083 0.0215 0.2232 0.0415 0.2732 0.0418 0.0321 0.4237 0.0462 0.0356 0.0073 0.1041 0.0212 0.0635 0.0300
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0906 0.7246 0.0121 0.0107 0.0251 0.2075 0.0408 0.2398 0.0416 0.0321 0.4464 0.0535 0.0382 0.0091 0.1052 0.0257 0.0789 0.0257
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0824 0.6452 0.0117 0.0105 0.0202 0.1957 0.0365 0.2457 0.0358 0.0299 0.4016 0.0423 0.0351 0.0082 0.0998 0.0243 0.0673 0.0282
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0888 0.7194 0.0130 0.0105 0.0295 0.2045 0.0398 0.2632 0.0365 0.0337 0.4184 0.0326 0.0398 0.0095 0.0963 0.0237 0.0702 0.0267
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0866 0.5988 0.0128 0.0100 0.0246 0.2359 0.0408 0.2564 0.0549 0.0362 0.4608 0.0518 0.0313 0.0107 0.1344 0.0277 0.0740 0.0311
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0906 0.6061 0.0139 0.0113 0.0355 0.2212 0.0430 0.2463 0.0570 0.0351 0.4926 0.0536 0.0335 0.0103 0.1531 0.0253 0.0770 0.0282
WM1552C 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0814 0.5525 0.0103 0.0094 0.0255 0.1789 0.0306 0.2123 0.0414 0.0271 0.3846 0.0343 0.0262 0.0081 0.0849 0.0181 0.0521 0.0300