Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0416 0.5556 0.0826 0.0439 0.0102 0.0116 0.0062 0.0088 0.0095 0.0425 0.0691 0.0413 0.1092 0.0255 0.0091 0.0074 0.0106 0.0202 0.0123 0.1357 0.0754
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0249 0.1961 0.0421 0.0151 0.0059 0.0051 0.0028 0.0042 0.0051 0.0643 0.0223 0.0177 0.0425 0.0103 0.0058 0.0036 0.0053 0.0062 0.0068 0.0882 0.0387
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0299 0.2577 0.0393 0.0175 0.0061 0.0068 0.0028 0.0049 0.0052 0.0416 0.0293 0.0215 0.0550 0.0115 0.0068 0.0039 0.0057 0.0098 0.0080 0.0925 0.0551
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0338 0.4329 0.0810 0.0404 0.0115 0.0161 0.0085 0.0119 0.0147 0.0393 0.0976 0.0507 0.1095 0.0264 0.0134 0.0093 0.0153 0.0245 0.0139 0.1181 0.0629
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0469 0.5814 0.1060 0.0527 0.0129 0.0171 0.0108 0.0138 0.0163 0.0426 0.1309 0.0539 0.1575 0.0349 0.0115 0.0096 0.0129 0.0310 0.0143 0.1692 0.0821
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0250 0.2227 0.0494 0.0225 0.0074 0.0083 0.0051 0.0104 0.0064 0.0308 0.0494 0.0253 0.0561 0.0149 0.0072 0.0051 0.0069 0.0112 0.0080 0.0828 0.0407
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0286 0.3472 0.0598 0.0264 0.0070 0.0086 0.0042 0.0061 0.0063 0.0332 0.0656 0.0275 0.0770 0.0142 0.0073 0.0045 0.0074 0.0134 0.0082 0.0976 0.0451
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0323 0.4951 0.0865 0.0388 0.0124 0.0153 0.0086 0.0117 0.0129 0.0365 0.0670 0.0491 0.1076 0.0274 0.0137 0.0126 0.0127 0.0245 0.0141 0.1048 0.0637
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0428 0.6452 0.1206 0.0482 0.0108 0.0146 0.0074 0.0124 0.0127 0.0415 0.0855 0.0545 0.1504 0.0286 0.0105 0.0074 0.0118 0.0264 0.0154 0.1368 0.0778
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0249 0.1894 0.0331 0.0139 0.0046 0.0048 0.0033 0.0053 0.0051 0.0541 0.0237 0.0197 0.0457 0.0113 0.0069 0.0036 0.0054 0.0090 0.0075 0.0732 0.0423
WM1552C 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0280 0.2398 0.0360 0.0136 0.0053 0.0055 0.0031 0.0051 0.0051 0.0524 0.0286 0.0248 0.0586 0.0112 0.0072 0.0042 0.0061 0.0090 0.0079 0.0937 0.0466
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0268 0.3040 0.0649 0.0302 0.0085 0.0114 0.0057 0.0091 0.0095 0.0282 0.0726 0.0356 0.0741 0.0197 0.0087 0.0059 0.0084 0.0152 0.0104 0.0943 0.0387
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0321 0.3953 0.0662 0.0336 0.0096 0.0123 0.0057 0.0078 0.0069 0.0304 0.0792 0.0363 0.1017 0.0210 0.0097 0.0047 0.0091 0.0161 0.0108 0.1081 0.0466
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0214 0.1385 0.0307 0.0147 0.0053 0.0047 0.0030 0.0045 0.0047 0.0346 0.0275 0.0188 0.0320 0.0090 0.0051 0.0036 0.0051 0.0055 0.0066 0.0642 0.0285
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0271 0.1832 0.0348 0.0167 0.0040 0.0058 0.0029 0.0036 0.0044 0.0343 0.0349 0.0247 0.0471 0.0098 0.0053 0.0032 0.0052 0.0067 0.0068 0.0712 0.0407
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0276 0.3472 0.0642 0.0279 0.0079 0.0100 0.0051 0.0083 0.0088 0.0248 0.0553 0.0355 0.0769 0.0183 0.0086 0.0060 0.0079 0.0141 0.0112 0.1073 0.0427
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0338 0.4926 0.0781 0.0347 0.0096 0.0094 0.0059 0.0065 0.0080 0.0286 0.0649 0.0383 0.1005 0.0194 0.0101 0.0048 0.0096 0.0159 0.0114 0.1202 0.0569
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0219 0.1675 0.0305 0.0123 0.0042 0.0042 0.0029 0.0045 0.0049 0.0211 0.0179 0.0344 0.0097 0.0052 0.0037 0.0044 0.0058 0.0066 0.0756 0.0298
WM1552C 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0265 0.2016 0.0337 0.0138 0.0047 0.0055 0.0032 0.0040 0.0049 0.0322 0.0281 0.0216 0.0469 0.0093 0.0067 0.0036 0.0045 0.0079 0.0070 0.0738 0.0456
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0210 0.1984 0.0485 0.0227 0.0059 0.0077 0.0058 0.0059 0.0266 0.0503 0.0242 0.0513 0.0130 0.0065 0.0045 0.0065 0.0099 0.0072 0.0812 0.0271
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0252 0.2336 0.0516 0.0261 0.0062 0.0078 0.0035 0.0046 0.0047 0.0286 0.0559 0.0228 0.0657 0.0135 0.0067 0.0033 0.0067 0.0089 0.0076 0.0876 0.0377
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0194 0.2212 0.0432 0.0176 0.0051 0.0059 0.0034 0.0051 0.0049 0.0308 0.0292 0.0229 0.0463 0.0102 0.0062 0.0042 0.0057 0.0074 0.0070 0.0698 0.0269
WM1552C 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0256 0.2710 0.0469 0.0224 0.0054 0.0069 0.0035 0.0052 0.0052 0.0334 0.0348 0.0216 0.0577 0.0115 0.0072 0.0035 0.0062 0.0095 0.0073 0.0756 0.0411