Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0276 0.3425 0.0759 0.0308 0.0114 0.0105 0.0082 0.0080 0.0081 0.0260 0.0519 0.0346 0.0767 0.0211 0.0084 0.0070 0.0086 0.0174 0.0101 0.0875 0.0509
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0312 0.5319 0.0870 0.0366 0.0109 0.0105 0.0055 0.0082 0.0075 0.0310 0.0673 0.0388 0.1095 0.0197 0.0086 0.0055 0.0089 0.0199 0.0104 0.1033 0.0628
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0283 0.1927 0.0301 0.0133 0.0044 0.0045 0.0032 0.0067 0.0049 0.0460 0.0264 0.0213 0.0436 0.0140 0.0067 0.0040 0.0061 0.0065 0.0077 0.0754 0.0548
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0275 0.3125 0.0336 0.0136 0.0050 0.0055 0.0029 0.0066 0.0070 0.0498 0.0338 0.0259 0.0575 0.0117 0.0070 0.0046 0.0059 0.0079 0.0086 0.0778 0.0571
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0303 0.3356 0.0709 0.0317 0.0099 0.0108 0.0057 0.0093 0.0094 0.0346 0.0765 0.0415 0.0737 0.0197 0.0086 0.0061 0.0079 0.0135 0.0103 0.0907 0.0411
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0338 0.4505 0.0772 0.0370 0.0102 0.0100 0.0063 0.0088 0.0087 0.0390 0.0894 0.0419 0.0969 0.0200 0.0073 0.0047 0.0097 0.0141 0.0110 0.1006 0.0494
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0223 0.1880 0.0345 0.0165 0.0044 0.0045 0.0032 0.0048 0.0042 0.0342 0.0344 0.0177 0.0375 0.0096 0.0053 0.0037 0.0048 0.0061 0.0060 0.0591 0.0286
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0257 0.1988 0.0403 0.0197 0.0046 0.0060 0.0033 0.0041 0.0048 0.0392 0.0464 0.0193 0.0517 0.0101 0.0054 0.0033 0.0047 0.0095 0.0059 0.0644 0.0430
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0307 0.4464 0.0774 0.0368 0.0096 0.0119 0.0060 0.0074 0.0098 0.0345 0.0616 0.0446 0.0880 0.0241 0.0120 0.0071 0.0104 0.0170 0.0122 0.1116 0.0508
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0371 0.6061 0.0935 0.0440 0.0126 0.0119 0.0068 0.0074 0.0124 0.0408 0.0752 0.0482 0.1217 0.0237 0.0089 0.0080 0.0118 0.0189 0.0135 0.1290 0.0709
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0201 0.1715 0.0306 0.0140 0.0050 0.0043 0.0029 0.0044 0.0040 0.0356 0.0220 0.0196 0.0368 0.0101 0.0052 0.0035 0.0048 0.0054 0.0064 0.0666 0.0295
WM1552C 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0253 0.2151 0.0370 0.0153 0.0065 0.0060 0.0035 0.0048 0.0056 0.0455 0.0312 0.0230 0.0523 0.0107 0.0071 0.0037 0.0051 0.0089 0.0073 0.0710 0.0447
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0303 0.3922 0.0713 0.0330 0.0105 0.0109 0.0062 0.0096 0.0085 0.0342 0.0789 0.0439 0.0813 0.0215 0.0101 0.0069 0.0087 0.0182 0.0106 0.1058 0.0443
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0367 0.4673 0.0729 0.0097 0.0120 0.0057 0.0077 0.0093 0.0369 0.0979 0.0394 0.1031 0.0241 0.0101 0.0050 0.0094 0.0161 0.0110 0.1175 0.0619
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0210 0.1996 0.0434 0.0206 0.0050 0.0057 0.0034 0.0049 0.0041 0.0345 0.0403 0.0212 0.0440 0.0118 0.0058 0.0043 0.0057 0.0072 0.0064 0.0737 0.0317
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0287 0.2404 0.0507 0.0272 0.0076 0.0089 0.0042 0.0052 0.0056 0.0351 0.0534 0.0226 0.0657 0.0127 0.0065 0.0046 0.0073 0.0102 0.0071 0.0796 0.0507
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0255 0.4292 0.0666 0.0301 0.0114 0.0086 0.0051 0.0080 0.0076 0.0354 0.0517 0.0373 0.0735 0.0192 0.0087 0.0063 0.0083 0.0145 0.0098 0.1053 0.0418
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0352 0.5405 0.0755 0.0411 0.0129 0.0116 0.0061 0.0098 0.0096 0.0400 0.0630 0.0393 0.0962 0.0212 0.0089 0.0053 0.0101 0.0175 0.0111 0.1146 0.0654
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0213 0.1901 0.0299 0.0135 0.0044 0.0044 0.0027 0.0038 0.0035 0.0224 0.0166 0.0361 0.0100 0.0055 0.0036 0.0049 0.0054 0.0060 0.0700 0.0357
WM1552C 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0311 0.2475 0.0419 0.0179 0.0060 0.0075 0.0031 0.0054 0.0058 0.0438 0.0333 0.0217 0.0595 0.0133 0.0086 0.0055 0.0050 0.0099 0.0074 0.0737 0.0553
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0295 0.3497 0.0580 0.0300 0.0087 0.0095 0.0052 0.0076 0.0082 0.0391 0.0693 0.0387 0.0693 0.0192 0.0083 0.0068 0.0082 0.0151 0.0106 0.0988 0.0433
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0431 0.4292 0.0611 0.0362 0.0091 0.0126 0.0056 0.0074 0.0084 0.0466 0.0808 0.0364 0.0890 0.0207 0.0099 0.0062 0.0091 0.0163 0.0118 0.1136 0.0622
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0235 0.1678 0.0323 0.0166 0.0048 0.0055 0.0030 0.0043 0.0041 0.0645 0.0296 0.0172 0.0372 0.0105 0.0061 0.0039 0.0054 0.0064 0.0068 0.0701 0.0352
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0294 0.2037 0.0351 0.0191 0.0051 0.0063 0.0032 0.0047 0.0046 0.0556 0.0368 0.0179 0.0545 0.0110 0.0055 0.0031 0.0055 0.0085 0.0074 0.0746 0.0479
WM1552C 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0310 0.4587 0.0728 0.0356 0.0097 0.0105 0.0059 0.0082 0.0085 0.0376 0.0562 0.0405 0.0768 0.0249 0.0097 0.0071 0.0100 0.0165 0.0109 0.1217 0.0503