Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0269 0.2257 0.0238 0.0124 0.0040 0.0064 0.0027 0.0037 0.0041 0.0940 0.0179 0.0137 0.0568 0.0102 0.0049 0.0026 0.0051 0.0055 0.0044 0.0697 0.0691
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0243 0.1965 0.0247 0.0111 0.0040 0.0054 0.0029 0.0040 0.0041 0.0194 0.0265 0.0441 0.0097 0.0057 0.0037 0.0064 0.0049 0.0045 0.0759 0.0690
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0302 0.2304 0.0267 0.0128 0.0041 0.0069 0.0028 0.0042 0.1068 0.0235 0.0296 0.0766 0.0119 0.0043 0.0031 0.0060 0.0064 0.0059 0.0838 0.0988
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0217 0.1642 0.0248 0.0114 0.0042 0.0066 0.0026 0.0038 0.0078 0.1217 0.0193 0.0400 0.0455 0.0094 0.0036 0.0051 0.0053 0.0051 0.1639 0.0592
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.1822 0.0258 0.0124 0.0074 0.0028 0.0037 0.0067 0.1159 0.0236 0.0302 0.0640 0.0108 0.0042 0.0036 0.0054 0.0059 0.0073 0.1825 0.0797
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0286 0.1639 0.0267 0.0133 0.0055 0.0044 0.0031 0.0044 0.0047 0.0860 0.0347 0.0195 0.0412 0.0092 0.0053 0.0055 0.0056 0.0069 0.0056 0.0745 0.0447
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0365 0.2183 0.0358 0.0162 0.0056 0.0044 0.0032 0.0047 0.0054 0.0837 0.0398 0.0210 0.0580 0.0085 0.0056 0.0056 0.0056 0.0081 0.0051 0.0797 0.0532
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0382 0.1754 0.0634 0.0141 0.0052 0.0039 0.0034 0.0042 0.0051 0.0476 0.0449 0.0558 0.0479 0.0102 0.0043 0.0046 0.0066 0.0088 0.0059 0.0898 0.0767
WM1552C 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0429 0.1961 0.0473 0.0161 0.0070 0.0045 0.0034 0.0089 0.0056 0.0394 0.0534 0.0452 0.0767 0.0118 0.0061 0.0059 0.0077 0.0100 0.0065 0.0879 0.0823
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0246 0.2364 0.0549 0.0274 0.0084 0.0078 0.0045 0.0065 0.0065 0.0222 0.0701 0.0223 0.0657 0.0125 0.0058 0.0049 0.0073 0.0093 0.0062 0.0730 0.0369
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0275 0.3086 0.0556 0.0316 0.0088 0.0080 0.0048 0.0062 0.0063 0.0243 0.0824 0.0225 0.0853 0.0121 0.0047 0.0039 0.0088 0.0094 0.0065 0.0790 0.0420
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0249 0.1410 0.0239 0.0102 0.0068 0.0036 0.0026 0.0042 0.0041 0.0485 0.0367 0.0118 0.0330 0.0063 0.0045 0.0035 0.0046 0.0044 0.0056 0.0688 0.0340
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0282 0.1887 0.0275 0.0123 0.0081 0.0049 0.0030 0.0043 0.0046 0.0436 0.0425 0.0134 0.0494 0.0077 0.0048 0.0031 0.0049 0.0057 0.0051 0.0716 0.0582
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0275 0.2439 0.0553 0.0301 0.0099 0.0058 0.0045 0.0067 0.0062 0.0193 0.0581 0.0256 0.0638 0.0137 0.0058 0.0065 0.0072 0.0093 0.0059 0.0755 0.0383
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0317 0.3165 0.0710 0.0382 0.0108 0.0071 0.0048 0.0044 0.0062 0.0217 0.0714 0.0273 0.0876 0.0116 0.0052 0.0076 0.0085 0.0103 0.0065 0.0835 0.0553
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0263 0.1506 0.0266 0.0136 0.0061 0.0034 0.0026 0.0041 0.0037 0.0688 0.0313 0.0191 0.0364 0.0077 0.0046 0.0049 0.0043 0.0045 0.0047 0.0743 0.0342
WM1552C 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0333 0.1972 0.0328 0.0153 0.0087 0.0049 0.0031 0.0047 0.0053 0.0612 0.0437 0.0224 0.0511 0.0104 0.0060 0.0051 0.0055 0.0080 0.0053 0.0703 0.0605
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0253 0.2717 0.0565 0.0257 0.0088 0.0076 0.0047 0.0061 0.0057 0.0252 0.0745 0.0264 0.0619 0.0129 0.0062 0.0058 0.0083 0.0114 0.0064 0.0939 0.0387
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0303 0.3226 0.0578 0.0319 0.0080 0.0079 0.0046 0.0054 0.0063 0.0287 0.0887 0.0231 0.0813 0.0141 0.0067 0.0043 0.0089 0.0114 0.0067 0.0980 0.0547