Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0182 0.1733 0.0231 0.0139 0.0041 0.0030 0.0028 0.0038 0.0034 0.0462 0.0188 0.0159 0.0410 0.0141 0.0038 0.0073 0.0040 0.0036 0.0033 0.0370 0.0374
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0233 0.2128 0.0274 0.0172 0.0057 0.0047 0.0031 0.0039 0.0047 0.0368 0.0267 0.0175 0.0596 0.0153 0.0042 0.0076 0.0049 0.0056 0.0034 0.0394 0.0627
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0210 0.2688 0.0370 0.0263 0.0057 0.0048 0.0036 0.0047 0.0044 0.0231 0.0313 0.0197 0.0543 0.0194 0.0048 0.0088 0.0055 0.0080 0.0046 0.0464 0.0481
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0271 0.3290 0.0429 0.0336 0.0068 0.0085 0.0039 0.0039 0.0054 0.0242 0.0361 0.0209 0.0840 0.0191 0.0044 0.0084 0.0069 0.0088 0.0050 0.0538 0.0760
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0296 0.5618 0.0735 0.0479 0.0268 0.0096 0.0070 0.0110 0.0099 0.0309 0.0725 0.0430 0.1130 0.0359 0.0108 0.0138 0.0100 0.0152 0.0090 0.0810 0.0832
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0413 0.6410 0.0824 0.0637 0.0266 0.0137 0.0084 0.0097 0.0112 0.0370 0.0829 0.0475 0.1567 0.0431 0.0108 0.0125 0.0128 0.0205 0.0105 0.0867 0.1264
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0206 0.2247 0.0273 0.0192 0.0055 0.0042 0.0031 0.0040 0.0041 0.0322 0.0251 0.0178 0.0535 0.0192 0.0047 0.0091 0.0052 0.0050 0.0044 0.0442 0.0553
WM1552C 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0289 0.2841 0.0370 0.0264 0.0067 0.0072 0.0039 0.0046 0.0061 0.0266 0.0352 0.0209 0.0822 0.0203 0.0051 0.0102 0.0063 0.0091 0.0046 0.0514 0.0845
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0239 0.3115 0.0444 0.0295 0.0074 0.0065 0.0055 0.0066 0.0059 0.0377 0.0393 0.0256 0.0695 0.0228 0.0056 0.0105 0.0068 0.0101 0.0056 0.0524 0.0540
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0351 0.3650 0.0470 0.0346 0.0064 0.0076 0.0042 0.0055 0.0060 0.0484 0.0429 0.0223 0.0936 0.0265 0.0062 0.0076 0.0071 0.0101 0.0057 0.0527 0.0720
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0262 0.2841 0.0416 0.0264 0.0072 0.0065 0.0045 0.0056 0.0059 0.0323 0.0360 0.0245 0.0645 0.0241 0.0058 0.0092 0.0068 0.0088 0.0053 0.0571 0.0605
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0321 0.3663 0.0488 0.0349 0.0072 0.0080 0.0042 0.0042 0.0059 0.0303 0.0443 0.0237 0.1043 0.0281 0.0043 0.0100 0.0077 0.0105 0.0054 0.0645 0.0891
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0349 0.5556 0.0804 0.0520 0.0260 0.0127 0.0071 0.0098 0.0111 0.0380 0.0724 0.0452 0.1280 0.0455 0.0100 0.0148 0.0122 0.0200 0.0091 0.0985 0.0916
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.6849 0.0959 0.0638 0.0247 0.0132 0.0073 0.0103 0.0105 0.0384 0.0912 0.0438 0.1754 0.0534 0.0087 0.0135 0.0125 0.0244 0.0100 0.1085 0.1290
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0247 0.2123 0.0283 0.0178 0.0036 0.0044 0.0030 0.0041 0.0046 0.0228 0.0177 0.0491 0.0191 0.0047 0.0073 0.0048 0.0058 0.0041 0.0461 0.0593
WM1552C 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0288 0.2364 0.0307 0.0187 0.0041 0.0049 0.0029 0.0036 0.0046 0.0385 0.0281 0.0192 0.0695 0.0199 0.0040 0.0089 0.0048 0.0069 0.0043 0.0509 0.0797
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0322 0.6803 0.0758 0.0375 0.0208 0.0170 0.0085 0.0112 0.0119 0.0237 0.0596 0.0458 0.1482 0.0282 0.0118 0.0078 0.0143 0.0228 0.0109 0.1218 0.1047
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0425 0.9524 0.1010 0.0505 0.0222 0.0180 0.0063 0.0128 0.0141 0.0285 0.0784 0.0543 0.2359 0.0309 0.0091 0.0070 0.0125 0.0252 0.0117 0.1629 0.1355
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0289 0.4386 0.0584 0.0288 0.0138 0.0096 0.0056 0.0067 0.0070 0.0167 0.0409 0.0314 0.0974 0.0198 0.0079 0.0052 0.0112 0.0121 0.0070 0.0930 0.0833
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0316 0.6757 0.0708 0.0343 0.0118 0.0125 0.0055 0.0078 0.0076 0.0184 0.0554 0.0370 0.1435 0.0199 0.0059 0.0048 0.0092 0.0147 0.0071 0.1088 0.1032
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0349 0.6803 0.0796 0.0416 0.0193 0.0256 0.0091 0.0127 0.0124 0.0298 0.0895 0.0547 0.1681 0.0334 0.0134 0.0098 0.0195 0.0257 0.0115 0.1259 0.1119
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0421 0.9524 0.1091 0.0531 0.0183 0.0270 0.0077 0.0146 0.0138 0.0323 0.1208 0.0611 0.2558 0.0312 0.0096 0.0065 0.0132 0.0303 0.0123 0.1563 0.1393
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0214 0.2494 0.0372 0.0190 0.0065 0.0084 0.0039 0.0061 0.0049 0.0184 0.0352 0.0227 0.0641 0.0120 0.0057 0.0039 0.0068 0.0066 0.0047 0.0698 0.0652