Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0453 0.7092 0.1002 0.0716 0.0521 0.0156 0.0081 0.0111 0.0117 0.0320 0.0750 0.0533 0.1721 0.0447 0.0120 0.0140 0.0135 0.0213 0.0099 0.1014 0.1431
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0195 0.2165 0.0249 0.0181 0.0050 0.0039 0.0029 0.0038 0.0039 0.0722 0.0208 0.0164 0.0502 0.0181 0.0046 0.0080 0.0046 0.0044 0.0040 0.0419 0.0518
WM1552C 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0283 0.2890 0.0370 0.0233 0.0064 0.0069 0.0036 0.0053 0.0070 0.0608 0.0299 0.0204 0.0751 0.0208 0.0061 0.0114 0.0058 0.0099 0.0044 0.0490 0.0821
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0410 0.6494 0.0896 0.0593 0.0531 0.0143 0.0093 0.0129 0.0153 0.0448 0.0822 0.0588 0.1515 0.0510 0.0130 0.0176 0.0166 0.0251 0.0114 0.0905 0.1104
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0573 0.8000 0.1013 0.0733 0.0541 0.0167 0.0093 0.0131 0.0148 0.0519 0.0986 0.0564 0.2008 0.0543 0.0128 0.0158 0.0173 0.0283 0.0128 0.1036 0.1423
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0281 0.3559 0.0592 0.0445 0.0121 0.0089 0.0057 0.0077 0.0082 0.0353 0.0459 0.0319 0.0837 0.0308 0.0088 0.0100 0.0082 0.0118 0.0064 0.0592 0.0623
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0367 0.4951 0.0675 0.0546 0.0133 0.0098 0.0056 0.0072 0.0080 0.0338 0.0613 0.0321 0.1376 0.0331 0.0053 0.0096 0.0091 0.0154 0.0059 0.0682 0.0950
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0344 0.5495 0.0862 0.0593 0.0308 0.0129 0.0076 0.0098 0.0111 0.0375 0.0713 0.0476 0.1214 0.0093 0.0131 0.0130 0.0184 0.0097 0.0797 0.0882
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0462 0.6329 0.1028 0.0786 0.0283 0.0150 0.0076 0.0090 0.0109 0.0411 0.0879 0.0488 0.1802 0.0481 0.0098 0.0113 0.0136 0.0242 0.0107 0.0907 0.1383
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0232 0.2262 0.0331 0.0219 0.0045 0.0052 0.0032 0.0047 0.0059 0.0206 0.0196 0.0515 0.0197 0.0049 0.0069 0.0061 0.0061 0.0043 0.0400 0.0497
WM1552C 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0262 0.2551 0.0425 0.0239 0.0049 0.0058 0.0028 0.0042 0.0057 0.0481 0.0278 0.0225 0.0772 0.0202 0.0048 0.0090 0.0059 0.0092 0.0040 0.0469 0.0687
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0437 0.7937 0.0930 0.0571 0.0241 0.0170 0.0121 0.0161 0.0181 0.0397 0.1052 0.0682 0.1776 0.0496 0.0151 0.0174 0.0171 0.0324 0.0141 0.1205 0.1138
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0592 1.0101 0.1018 0.0711 0.0286 0.0187 0.0086 0.0163 0.0167 0.0386 0.1355 0.0791 0.2725 0.0661 0.0101 0.0234 0.0175 0.0393 0.0140 0.1553 0.1337
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0281 0.3571 0.0560 0.0386 0.0109 0.0081 0.0060 0.0066 0.0070 0.0200 0.0497 0.0303 0.0965 0.0306 0.0068 0.0092 0.0088 0.0135 0.0060 0.0532 0.0643
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0311 0.5102 0.0686 0.0475 0.0114 0.0092 0.0061 0.0076 0.0078 0.0208 0.0653 0.0341 0.1466 0.0303 0.0051 0.0114 0.0089 0.0140 0.0063 0.0646 0.0830
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0386 0.6579 0.0855 0.0507 0.0208 0.0156 0.0096 0.0111 0.0139 0.0305 0.0822 0.0552 0.1667 0.0460 0.0129 0.0148 0.0170 0.0248 0.0118 0.0909 0.1164
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0490 0.8547 0.1038 0.0661 0.0248 0.0128 0.0090 0.0137 0.0140 0.0332 0.1093 0.0657 0.2463 0.0514 0.0121 0.0187 0.0147 0.0302 0.0120 0.1161 0.1464
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0210 0.2079 0.0321 0.0173 0.0085 0.0039 0.0034 0.0042 0.0042 0.0229 0.0220 0.0181 0.0532 0.0177 0.0047 0.0074 0.0050 0.0077 0.0040 0.0428 0.0581
WM1552C 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0233 0.2494 0.0332 0.0190 0.0096 0.0044 0.0033 0.0044 0.0045 0.0209 0.0298 0.0211 0.0721 0.0174 0.0044 0.0089 0.0046 0.0061 0.0037 0.0458 0.0705
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0427 0.6944 0.0869 0.0539 0.0229 0.0153 0.0089 0.0186 0.0157 0.0332 0.0977 0.0608 0.1524 0.0497 0.0139 0.0187 0.0122 0.0260 0.0120 0.1177 0.1044
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0447 0.8696 0.0791 0.0598 0.0216 0.0131 0.0098 0.0124 0.0106 0.0343 0.0999 0.0607 0.2037 0.0487 0.0135 0.0174 0.0140 0.0228 0.0113 0.1224 0.1170
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0255 0.3333 0.0538 0.0336 0.0078 0.0056 0.0049 0.0059 0.0057 0.0166 0.0429 0.0263 0.0783 0.0250 0.0061 0.0104 0.0068 0.0080 0.0052 0.0581 0.0527
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0301 0.4132 0.0602 0.0410 0.0082 0.0092 0.0051 0.0045 0.0065 0.0169 0.0582 0.0279 0.1106 0.0267 0.0052 0.0110 0.0089 0.0094 0.0048 0.0685 0.0916
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0260 0.3745 0.0636 0.0411 0.0089 0.0096 0.0065 0.0080 0.0084 0.0280 0.0594 0.0362 0.1095 0.0338 0.0076 0.0110 0.0089 0.0093 0.0070 0.0635 0.0547
WM1552C 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0313 0.5076 0.0666 0.0545 0.0111 0.0087 0.0063 0.0052 0.0078 0.0304 0.0714 0.0368 0.1464 0.0339 0.0085 0.0104 0.0105 0.0155 0.0078 0.0660 0.0799