Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.4237 7.6923 0.0702 0.1110 0.3534 0.1873 0.4902 0.1391 0.3279 0.1779 0.1560 0.3333 0.3311 0.0983 0.2833 0.1290
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.3831 3.4483 0.0431 0.1209 0.5495 0.1957 0.6135 0.1306 0.3333 0.2092 0.1282 0.2096 0.3077 0.0867 0.2976 0.1121
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.4444 7.1429 0.0693 0.1181 0.5917 0.2294 0.5988 0.1319 0.3012 0.1845 0.1425 0.2247 0.3345 0.0991 0.3165 0.1664
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.4926 4.3478 0.0579 0.1185 0.3378 0.1838 0.5618 0.1852 0.3906 0.2092 0.1258 0.3268 0.4184 0.0903 0.2732 0.1127
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.4951 7.6923 0.0700 0.1135 0.3584 0.2079 0.5682 0.1764 0.3610 0.2387 0.1626 0.3677 0.3953 0.1091 0.2857 0.1119
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.4525 7.1429 0.0508 0.1185 0.4082 0.1969 0.5988 0.1409 0.4098 0.2404 0.1534 0.2985 0.4717 0.0949 0.3333 0.1325
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.5051 8.3333 0.0944 0.1026 0.4202 0.2532 0.6536 0.1661 0.3802 0.2268 0.1919 0.2273 0.3571 0.0951 0.3509 0.1931
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 8.3333 0.0492 0.1304 0.4808 0.2101 0.6623 0.1730 0.4425 0.2294 0.1196 0.3165 0.4310 0.0966 0.3040
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4739 9.0909 0.0527 0.1372 0.4444 0.2404 0.5525 0.1678 0.4975 0.1980 0.1618 0.3846 0.3876 0.1027 0.3012 0.1412
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4695 7.6923 0.1082 0.2058 0.5988 0.2538 0.4219 0.1992 0.7092 0.2198 0.2079 0.6579 0.4484 0.1410 0.3106 0.1550
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4831 7.6923 0.1374 0.2392 0.5917 0.2833 0.3968 0.1919 0.6369 0.2008 0.2155 0.6452 0.5319 0.3215
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4975 7.6923 0.0504 0.1447 0.4167 0.1795 0.5556 0.1832 0.5263 0.2381 0.1355 0.3425 0.4546 0.0851 0.3030 0.1534
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.5155 8.3333 0.0623 0.1486 0.3846 0.2359 0.5128 0.1908 0.5000 0.1876 0.1482 0.3484 0.4082 0.1064 0.3077 0.1541
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4878 8.3333 0.0518 0.1319 0.4878 0.2169 0.5263 0.1572 0.5102 0.1949 0.1359 0.2577 0.3448 0.0859 0.3185 0.1548
C32 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.4854 7.1429 0.0781 0.1403 0.4785 0.2532 0.4762 0.1695 0.4587 0.1815 0.1451 0.2632 0.4902 0.0991 0.3333 0.1718
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.3650 7.6923 0.0412 0.1245 0.4219 0.1919 0.5587 0.0813 0.4219 0.1695 0.1103 0.3311 0.3584 0.1056 0.3030 0.1168
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.3802 0.0696 0.1376 0.4016 0.2053 0.3906 0.1608 0.4202 0.1736 0.1372 0.1984 0.3367 0.0818 0.2941 0.1282
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.3571 7.6923 0.0406 0.1227 0.6250 0.2008 0.5682 0.1256 0.4484 0.1618 0.1192 0.2165 0.4274 0.0838 0.2857 0.1337
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.3774 7.1429 0.0661 0.1520 0.5917 0.2283 0.5208 0.1437 0.4184 0.1848 0.1546 0.1773 0.3802 0.0912 0.2994 0.1477
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 7.6923 0.0499 0.1330 0.4546 0.2155 0.5618 0.1582 0.5025 0.1789 0.1215 0.3390 0.4695 0.0759 0.3300 0.1311
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.4032 9.0909 0.0467 0.1227 0.3876 0.2242 0.4630 0.2008 0.4975 0.1957 0.1407 0.2882 0.3774 0.1091 0.3300 0.1423
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.3774 7.6923 0.0481 0.4425 0.2294 0.4695 0.1287 0.5181 0.1447 0.1403 0.2500 0.2857 0.0915 0.3215 0.1477
C32 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.4049 6.6667 0.0760 0.1129 0.4167 0.2273 0.5076 0.1410 0.4951 0.1692 0.1592 0.2123 0.3436 0.0895 0.3401 0.1658
C32 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.3484 7.6923 0.0411 0.1416 0.4464 0.1825 0.5236 0.3597 0.1727 0.1010 0.3922 0.3472 0.0822 0.3040 0.1445
C32 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.3891 9.0909 0.0394 0.1407 0.4348 0.1976 0.4831 0.1590 0.3968 0.2033 0.1443 0.3436 0.4464 0.0843 0.3106 0.1271