Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4630 9.0909 0.0427 0.1276 0.5465 0.2101 0.6410 0.1565 0.4695 0.2268 0.1272 0.3650 0.3623 0.0964 0.2618 0.1070
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4878 9.0909 0.0573 0.1454 0.4831 0.2416 0.5618 0.1451 0.5155 0.2037 0.1675 0.3677 0.3759 0.1019 0.2786 0.1368
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4386 7.6923 0.0480 0.1484 0.6452 0.5587 0.1214 0.4717 0.2137 0.1631 0.2088 0.3030 0.0861 0.2375 0.1263
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4484 7.6923 0.0740 0.1634 0.6667 0.2506 0.6061 0.1212 0.4425 0.1770 0.1520 0.2227 0.3497 0.1074 0.2500 0.1495
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4926 8.3333 0.0741 0.1318 0.4808 0.2227 0.6667 0.1828 0.5495 0.2232 0.1504 0.3290 0.3731 0.1100 0.3003 0.1887
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.5025 7.6923 0.0670 0.1326 0.4566 0.2538 0.5747 0.1901 0.4902 0.1873 0.1669 0.3049 0.4098 0.1091 0.2967 0.1575
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4785 9.0909 0.0532 0.1259 0.5076 0.2445 0.5747 0.1508 0.5376 0.2004 0.1595 0.2890 0.3279 0.1220 0.2882 0.1475
C32 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.4717 7.6923 0.0879 0.1403 0.4808 0.2481 0.5814 0.1812 0.4902 0.1802 0.1486 0.2841 0.4065 0.1011 0.3106
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.3759 8.3333 0.0431 0.1300 0.4975 0.1876 0.6623 0.1616 0.4566 0.1595 0.1159 0.3425 0.3953 0.0840 0.2865 0.1119
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.3953 10.0000 0.0549 0.1454 0.4762 0.2004 0.5291 0.1425 0.4762 0.1792 0.1600 0.3175 0.3610 0.0777 0.2857 0.1449
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4329 7.6923 0.1325 0.2710 0.6536 0.2833 0.4098 0.2416 0.7634 0.2004 0.1992 0.5587 0.1115 0.2695 0.2283
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4630 7.1429 0.1462 0.2416 0.6494 0.2976 0.4000 0.3003 0.7407 0.2410 0.6289 0.5348 0.2976 0.2208
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4167 9.0909 0.0500 0.1912 0.4902 0.2538 0.5814 0.2252 0.6452 0.1758 0.1667 0.3472 0.6993 0.0725 0.3049 0.1321
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4049 9.0909 0.0608 0.1541 0.4386 0.1678 0.5155 0.2132 0.6250 0.2123 0.2041 0.3125 0.5814 0.0903 0.3106 0.1570
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4219 9.0909 0.0625 0.1211 0.5587 0.2075 0.5076 0.1949 0.5682 0.1866 0.1862 0.3745 0.5556 0.0702 0.3472 0.1610
C32 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.4505 7.1429 0.1104 0.1815 0.4149 0.2762 0.5348 0.2062 0.6329 0.2347 0.1905 0.2809 0.6494 0.1163 0.3597 0.1852
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.3521 8.3333 0.0372 0.1198 0.4854 0.2037 0.5714 0.1786 0.3636 0.1656 0.1277 0.5988 0.4310 0.1042 0.3226 0.1575
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.4484 8.3333 0.0579 0.1223 0.4566 0.1953 0.4854 0.1684 0.2732 0.2151 0.1621 0.3058 0.3846 0.0864 0.3145 0.1153
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.4082 7.6923 0.0992 0.3953 0.6098 0.2710 0.4065 0.2227 0.6329 0.1792 0.2053 0.8265 0.5102 0.1490 0.3322 0.1727
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.4831 6.6667 0.1449 0.3690 0.6250 0.2506 0.4292 0.2222 0.6369 0.2591 0.2577 0.5236 0.5495 0.3378
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.3876 7.1429 0.0395 0.1245 0.3968 0.2304 0.5208 0.2174 0.4184 0.1873 0.1306 0.3401 0.4831 0.1103 0.3559 0.1401
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.5076 8.3333 0.0845 0.1314 0.4386 0.2364 0.5051 0.2247 0.4386 0.2288 0.1908 0.2976 0.5525 0.1164 0.3718 0.1546
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.3953 9.0909 0.0441 0.1306 0.4032 0.2299 0.5495 0.1695 0.4115 0.2128 0.1383 0.3509 0.3984 0.1058 0.3021 0.1555
C32 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.5236 7.6923 0.1003 0.1139 0.4184 0.2481 0.5747 0.1835 0.4651 0.2342 0.1845 0.2747 0.4587 0.1107 0.3049 0.1724
C32 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.3953 4.1667 0.0528 0.1203 0.3922 0.1838 0.5405 0.1449 0.3268 0.2188 0.1277 0.2770 0.3096 0.2874 0.1094