Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.0494 0.3571 0.0112 0.0098 0.1089 0.0457 0.1319 0.0238 0.0283 0.0412 0.0365 0.0158 0.0219 0.0286 0.0661 0.0225
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0668 0.3906 0.0069 0.0090 0.0794 0.0387 0.0992 0.0154 0.0234 0.0337 0.0295 0.0189 0.0234 0.0342 0.0816 0.0172
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0778 0.4425 0.0099 0.0106 0.0834 0.0445 0.1095 0.0172 0.0283 0.0342 0.0198 0.0192 0.0301 0.0775 0.0211
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0751 0.3333 0.0077 0.0095 0.0798 0.0417 0.0961 0.0146 0.0220 0.0378 0.0312 0.0148 0.0203 0.0370 0.0797 0.0182
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0796 0.4292 0.0099 0.0106 0.0841 0.0426 0.0996 0.0182 0.0231 0.0365 0.0303 0.0159 0.0225 0.0337 0.0787 0.0205
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0749 0.3774 0.0066 0.0087 0.0694 0.0386 0.0866 0.0136 0.0210 0.0347 0.0217 0.0139 0.0186 0.0362 0.0841 0.0204
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0771 0.4464 0.0092 0.0092 0.0708 0.0423 0.0926 0.0155 0.0235 0.0339 0.0256 0.0148 0.0178 0.0333 0.0914 0.0221
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0603 0.2660 0.0067 0.0064 0.0654 0.0352 0.0573 0.0117 0.0217 0.0250 0.0205 0.0129 0.0109 0.0306 0.0638 0.0286
WM-115 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.0623 0.3185 0.0079 0.0068 0.0751 0.0314 0.0638 0.0141 0.0224 0.0269 0.0193 0.0142 0.0127 0.0361 0.0580 0.0250
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0694 0.3559 0.0071 0.0090 0.0704 0.0350 0.0959 0.0189 0.0226 0.0346 0.0319 0.0267 0.0301 0.0321 0.0774 0.0285
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0756 0.3861 0.0070 0.0088 0.0833 0.0366 0.1089 0.0180 0.0265 0.0314 0.0341 0.0181 0.0222 0.0311 0.0986 0.0151
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0713 0.3195 0.0092 0.0090 0.0716 0.0383 0.0913 0.0166 0.0312 0.0344 0.0322 0.0168 0.0235 0.0310 0.0998 0.0202
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0688 0.3937 0.0096 0.0098 0.0785 0.0409 0.0994 0.0205 0.0264 0.0340 0.0343 0.0183 0.0254 0.0283 0.0885 0.0218
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0573 0.3115 0.0102 0.0097 0.0724 0.0344 0.0704 0.0108 0.0198 0.0329 0.0240 0.0237 0.0184 0.0374 0.0983 0.0209
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0556 0.3663 0.0106 0.0100 0.0826 0.0354 0.0838 0.0124 0.0218 0.0327 0.0270 0.0188 0.0187 0.0334 0.1066 0.0198
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0490 0.2326 0.0091 0.0067 0.0697 0.0291 0.0618 0.0173 0.0200 0.0246 0.0222 0.0165 0.0106 0.0343 0.0787 0.0253
WM-115 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0471 0.2882 0.0096 0.0077 0.0801 0.0326 0.0624 0.0119 0.0198 0.0266 0.0210 0.0185 0.0138 0.0304 0.0760 0.0215
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0648 0.3268 0.0092 0.0626 0.0334 0.0959 0.0176 0.0233 0.0298 0.0286 0.0241 0.0238 0.0310 0.0990 0.0254
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0679 0.3571 0.0078 0.0089 0.0850 0.0372 0.1044 0.0185 0.0245 0.0324 0.0339 0.0196 0.0202 0.0298 0.1022 0.0158
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0684 0.3021 0.0082 0.0088 0.0728 0.0320 0.0864 0.0145 0.0213 0.0329 0.0310 0.0197 0.0195 0.0320 0.0965 0.0180
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0665 0.3597 0.0102 0.0095 0.0829 0.0406 0.1016 0.0198 0.0249 0.0339 0.0351 0.0182 0.0238 0.0307 0.0876 0.0203
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0659 0.3401 0.0076 0.0670 0.0335 0.0877 0.0159 0.0233 0.0291 0.0317 0.0234 0.0221 0.0303 0.0935 0.0257
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0618 0.3817 0.0087 0.0106 0.0844 0.0377 0.0991 0.0184 0.0258 0.0317 0.0351 0.0187 0.0206 0.0302 0.0936 0.0197
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0760 0.2994 0.0084 0.0081 0.0635 0.0315 0.0800 0.0154 0.0206 0.0325 0.0304 0.0150 0.0339 0.0767 0.0184
WM-115 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0731 0.3922 0.0097 0.0092 0.0763 0.0401 0.0974 0.0201 0.0253 0.0348 0.0302 0.0160 0.0201 0.0350 0.0712 0.0232