Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0574 0.3759 0.0059 0.0074 0.0640 0.0460 0.0992 0.0263 0.0270 0.0430 0.0333 0.0256 0.0162 0.0231 0.0396 0.0262
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0558 0.3322 0.0049 0.0427 0.0271 0.0905 0.0252 0.0476 0.0305 0.0269 0.0185 0.0227 0.0505 0.0215
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.3802 0.0074 0.0080 0.0588 0.0374 0.0907 0.0229 0.0300 0.0550 0.0337 0.0280 0.0220 0.0254 0.0334 0.0228
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.3817 0.0061 0.0061 0.0604 0.0447 0.0237 0.0253 0.0460 0.0299 0.0257 0.0133 0.0406 0.0297
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0584 0.3268 0.0039 0.0065 0.0530 0.0336 0.0974 0.0170 0.0215 0.0499 0.0266 0.0237 0.0212 0.0290 0.0282
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0715 0.4049 0.0054 0.0086 0.0529 0.0328 0.1033 0.0175 0.0297 0.0415 0.0272 0.0247 0.0105 0.0253 0.0320 0.0194
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0772 0.3521 0.0052 0.0517 0.0360 0.1006 0.0192 0.0231 0.0524 0.0262 0.0230 0.0172 0.0324 0.0539 0.0204
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0726 0.3802 0.0077 0.0099 0.0393 0.0229 0.0249 0.0449 0.0270 0.0229 0.0171 0.0301 0.0368 0.0192
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0655 0.3534 0.0036 0.0083 0.0468 0.0293 0.1026 0.0173 0.0281 0.0628 0.0234 0.0223 0.0336 0.0381 0.0165
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0676 0.4184 0.0067 0.0095 0.0515 0.0410 0.1011 0.0196 0.0290 0.0497 0.0303 0.0258 0.0165 0.0319 0.0418 0.0174
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0625 0.3356 0.0047 0.0081 0.0465 0.0371 0.0991 0.0191 0.0220 0.0407 0.0279 0.0212 0.0147 0.0275 0.0423 0.0200
WM-115 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0672 0.3802 0.0066 0.0088 0.0510 0.0407 0.1023 0.0211 0.0208 0.0403 0.0252 0.0206 0.0178 0.0215 0.0213 0.0204
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0666 0.3155 0.0074 0.0525 0.0309 0.0929 0.0157 0.0237 0.0500 0.0223 0.0198 0.0291 0.0312
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0714 0.3571 0.0049 0.0089 0.0588 0.0321 0.1063 0.0154 0.0242 0.0419 0.0276 0.0190 0.0133 0.0258 0.0450
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0672 0.3086 0.0053 0.0077 0.0553 0.0336 0.0968 0.0182 0.0238 0.0456 0.0258 0.0291 0.0172 0.0288 0.0435 0.0203
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0625 0.3677 0.0061 0.0069 0.0660 0.0406 0.1155 0.0220 0.0251 0.0420 0.0288 0.0238 0.0176 0.0271 0.0509 0.0204
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0694 0.3215 0.0050 0.0071 0.0485 0.0303 0.0929 0.0191 0.0254 0.0514 0.0271 0.0208 0.0314 0.0339 0.0197
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0685 0.3534 0.0054 0.0082 0.0494 0.0381 0.1086 0.0253 0.0468 0.0284 0.0267 0.0177 0.0289 0.0401 0.0168
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0804 0.3155 0.0050 0.0080 0.0468 0.0368 0.0951 0.0183 0.0217 0.0408 0.0258 0.0214 0.0128 0.0273 0.0407 0.0204
WM-115 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0749 0.3774 0.0061 0.0089 0.0541 0.0396 0.0995 0.0231 0.0222 0.0407 0.0285 0.0202 0.0169 0.0262 0.0427 0.0189
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0591 0.2985 0.0043 0.0060 0.0467 0.0325 0.0914 0.0194 0.0204 0.0403 0.0243 0.0301 0.0163 0.0241 0.0515
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0624 0.3290 0.0050 0.0079 0.0596 0.0358 0.1053 0.0250 0.0238 0.0422 0.0281 0.0241 0.0114 0.0244 0.0383 0.0138
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0673 0.2841 0.0047 0.0074 0.0489 0.0314 0.1016 0.0197 0.0210 0.0372 0.0304 0.0289 0.0153 0.0259 0.0478 0.0181
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0611 0.3401 0.0057 0.0064 0.0598 0.0384 0.1179 0.0251 0.0242 0.0394 0.0296 0.0244 0.0184 0.0254 0.0355 0.0181