Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0315 0.2326 0.0084 0.0389 0.0193 0.0413 0.0227 0.0253 0.0609 0.0204 0.0255 0.0170 0.0245 0.0420 0.0246
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0344 0.2519 0.0044 0.0061 0.0541 0.0257 0.0572 0.0157 0.0261 0.0629 0.0236 0.0290 0.0266 0.0196 0.0124
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0448 0.2725 0.0035 0.0077 0.0437 0.0272 0.0698 0.0149 0.0229 0.0426 0.0219 0.0214 0.0157 0.0241 0.0413 0.0134
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0396 0.3040 0.0055 0.0060 0.0540 0.0352 0.0829 0.0172 0.0243 0.0454 0.0292 0.0180 0.0173 0.0255 0.0166
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0434 0.2519 0.0041 0.0059 0.0368 0.0257 0.0779 0.0155 0.0259 0.0557 0.0260 0.0174 0.0161 0.0260 0.0440 0.0172
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0400 0.2874 0.0051 0.0062 0.0476 0.0326 0.0858 0.0186 0.0560 0.0294 0.0159 0.0182 0.0252 0.0260 0.0157
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
WM-115 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0416 0.2667 0.0056 0.0073 0.0476 0.0346 0.0852 0.0202 0.0246 0.0530 0.0266 0.0149 0.0152 0.0263 0.0282 0.0187
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0348 0.2564 0.0028 0.0073 0.0204 0.0200 0.0606 0.0222 0.0164 0.0232 0.0376
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0357 0.2976 0.0048 0.0065 0.0508 0.0305 0.0660 0.0163 0.0246 0.0645 0.0249 0.0155 0.0184 0.0232 0.0285 0.0177
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0409 0.2732 0.0038 0.0068 0.0431 0.0287 0.0716 0.0145 0.0225 0.0497 0.0255 0.0142 0.0142 0.0232 0.0345 0.0147
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0348 0.2907 0.0061 0.0077 0.0519 0.0300 0.0746 0.0190 0.0252 0.0569 0.0270 0.0153 0.0178 0.0228 0.0274 0.0157
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0345 0.2778 0.0016 0.0062 0.0333 0.0237 0.0664 0.0159 0.0268 0.0605 0.0241 0.0156 0.0189 0.0228 0.0435 0.0173
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0351 0.3086 0.0060 0.0087 0.0441 0.0296 0.0781 0.0198 0.0243 0.0693 0.0307 0.0159 0.0194 0.0275 0.0187 0.0165
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
WM-115 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0400 0.3012 0.0056 0.0079 0.0552 0.0365 0.0715 0.0188 0.0284 0.0618 0.0274 0.0166 0.0147 0.0281 0.0216 0.0192
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0379 0.2283 0.0035 0.0062 0.0456 0.0219 0.0744 0.0175 0.0199 0.0635 0.0215 0.0141 0.0182 0.0308 0.0426
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0456 0.2801 0.0050 0.0085 0.0492 0.0228 0.0139 0.0242 0.0501 0.0233 0.0099 0.0249 0.0233
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0438 0.2571 0.0038 0.0073 0.0470 0.0815 0.0126 0.0209 0.0623 0.0227 0.0109 0.0162 0.0318 0.0435 0.0161
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0501 0.2985 0.0067 0.0069 0.0532 0.0318 0.0792 0.0171 0.0240 0.0546 0.0255 0.0140 0.0180 0.0279 0.0261 0.0152
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0442 0.2632 0.0028 0.0074 0.0414 0.0257 0.0771 0.0130 0.0229 0.0741 0.0212 0.0154 0.0183 0.0337 0.0480 0.0149
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0499 0.3155 0.0045 0.0082 0.0482 0.0294 0.0803 0.0156 0.0239 0.0607 0.0211 0.0147 0.0190 0.0274 0.0318 0.0159
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0449 0.2506 0.0035 0.0077 0.0404 0.0277 0.0760 0.0132 0.0195 0.0491 0.0261 0.0119 0.0149 0.0227 0.0243 0.0160
WM-115 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0465 0.3049 0.0060 0.0101 0.0445 0.0307 0.0726 0.0156 0.0181 0.0451 0.0214 0.0133 0.0181 0.0207 0.0202 0.0161
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0427 0.2463 0.0464 0.0260 0.0757 0.0142 0.0570 0.0241 0.0278 0.0208 0.0294 0.0436 0.0259