Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0477 0.3058 0.0050 0.0086 0.0799 0.0334 0.0858 0.0178 0.0264 0.0623 0.0275 0.0159 0.0185 0.0244 0.0247 0.0150
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0509 0.2849 0.0038 0.0066 0.0525 0.0255 0.0614 0.0132 0.0190 0.0589 0.0263 0.0162 0.0166 0.0251 0.0365
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0477 0.3165 0.0045 0.0080 0.0723 0.0336 0.0719 0.0146 0.0240 0.0633 0.0281 0.0199 0.0119 0.0224 0.0271 0.0181
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0499 0.2849 0.0042 0.0074 0.0552 0.0319 0.0702 0.0133 0.0194 0.0546 0.0263 0.0165 0.0143 0.0224 0.0450 0.0172
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0447 0.3086 0.0059 0.0077 0.0730 0.0784 0.0159 0.0217 0.0593 0.0294 0.0195 0.0170 0.0225 0.0320 0.0168
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0442 0.2809 0.0036 0.0087 0.0537 0.0295 0.0659 0.0166 0.0191 0.0574 0.0278 0.0119 0.0176 0.0208 0.0401 0.0169
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0430 0.3257 0.0048 0.0087 0.0730 0.0348 0.0723 0.0192 0.0224 0.0633 0.0336 0.0158 0.0163 0.0236 0.0242 0.0181
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0526 0.2786 0.0045 0.0072 0.0540 0.0292 0.0617 0.0247 0.0183 0.0591 0.0260 0.0134 0.0111 0.0236 0.0322 0.0174
WM-115 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0492 0.3077 0.0055 0.0063 0.0706 0.0366 0.0810 0.0210 0.0222 0.0652 0.0311 0.0171 0.0156 0.0245 0.0286 0.0190
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0403 0.2247 0.0033 0.0076 0.0413 0.0248 0.0693 0.0138 0.0147 0.0520 0.0226 0.0160 0.0133 0.0271 0.0362 0.0182
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0487 0.2632 0.0052 0.0079 0.0488 0.0315 0.0860 0.0140 0.0204 0.0411 0.0229 0.0113 0.0101 0.0213 0.0192 0.0145
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0588 0.2288 0.0041 0.0055 0.0420 0.0310 0.0597 0.0124 0.0175 0.0493 0.0206 0.0111 0.0076 0.0279 0.0323 0.0188
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0583 0.2611 0.0066 0.0466 0.0343 0.0640 0.0172 0.0199 0.0416 0.0247 0.0128 0.0103 0.0270 0.0254 0.0173
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0383 0.1883 0.0037 0.0059 0.0364 0.0230 0.0632 0.0134 0.0170 0.0645 0.0170 0.0133 0.0136 0.0289 0.0174 0.0159
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0422 0.2169 0.0064 0.0063 0.0428 0.0297 0.0780 0.0157 0.0193 0.0548 0.0240 0.0120 0.0118 0.0239 0.0206 0.0161
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0344 0.1466 0.0041 0.0050 0.0349 0.0245 0.0579 0.0130 0.0136 0.0456 0.0244 0.0081 0.0064 0.0215 0.0256 0.0189
WM-115 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0368 0.1681 0.0053 0.0070 0.0378 0.0269 0.0546 0.0138 0.0165 0.0468 0.0207 0.0100 0.0139 0.0218 0.0464 0.0208
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0404 0.2770 0.0044 0.0072 0.0456 0.0222 0.0704 0.0126 0.0585 0.0221 0.0268 0.0194 0.0302 0.0444 0.0225
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0442 0.3125 0.0042 0.0076 0.0556 0.0272 0.0821 0.0127 0.0265 0.0549 0.0264 0.0157 0.0144 0.0241 0.0402 0.0133
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0466 0.2841 0.0047 0.0078 0.0478 0.0287 0.0791 0.0125 0.0237 0.0507 0.0251 0.0184 0.0176 0.0245 0.0298 0.0153
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0399 0.3448 0.0054 0.0090 0.0553 0.0352 0.0785 0.0170 0.0272 0.0469 0.0271 0.0181 0.0187 0.0229 0.0378 0.0126
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0441 0.2088 0.0041 0.0065 0.0411 0.0232 0.0812 0.0127 0.0210 0.0690 0.0233 0.0269 0.0157 0.0311 0.0206 0.0264
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0451 0.2475 0.0044 0.0054 0.0465 0.0286 0.0892 0.0172 0.0201 0.0590 0.0226 0.0139 0.0160 0.0249 0.0270 0.0138
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
WM-115 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0426 0.2347 0.0049 0.0064 0.0448 0.0301 0.0909 0.0169 0.0209 0.0493 0.0200 0.0115 0.0150 0.0239 0.0349 0.0200