Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.3356 0.0032 0.0069 0.0640 0.0298 0.0742 0.0236 0.0229 0.0667 0.0282 0.0138 0.0143 0.0227 0.0397 0.0162
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0434 0.3484 0.0066 0.0094 0.0873 0.0378 0.0991 0.0216 0.0290 0.0814 0.0319 0.0152 0.0162 0.0274 0.0294 0.0189
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0452 0.2857 0.0038 0.0088 0.0768 0.0277 0.0707 0.0143 0.0212 0.0804 0.0225 0.0141 0.0187 0.0305 0.0270
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0545 0.3534 0.0046 0.0116 0.0765 0.0281 0.0709 0.0138 0.0260 0.0637 0.0255 0.0144 0.0220 0.0225
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0495 0.2770 0.0037 0.0081 0.0306 0.0847 0.0152 0.0223 0.0773 0.0257 0.0133 0.0157 0.0327 0.0400 0.0165
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0533 0.3436 0.0060 0.0078 0.0743 0.0355 0.0847 0.0175 0.0228 0.0706 0.0250 0.0144 0.0168 0.0278 0.0268 0.0149
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0523 0.3205 0.0043 0.0164 0.0779 0.0281 0.0835 0.0147 0.0239 0.0906 0.0228 0.0190 0.0236 0.0322 0.0458 0.0155
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0519 0.3663 0.0058 0.0130 0.0845 0.0330 0.0906 0.0155 0.0275 0.0775 0.0264 0.0182 0.0187 0.0309 0.0276 0.0149
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0460 0.3077 0.0052 0.0094 0.0715 0.0315 0.0876 0.0139 0.0205 0.0644 0.0296 0.0159 0.0182 0.0262 0.0266 0.0138
WM-115 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.0503 0.3484 0.0063 0.0089 0.0786 0.0306 0.0881 0.0154 0.0210 0.0631 0.0232 0.0172 0.0206 0.0223 0.0392 0.0150
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0473 0.2725 0.0095 0.0666 0.0264 0.0750 0.0108 0.0793 0.0246 0.0324 0.0200 0.0294 0.0249
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0538 0.3155 0.0041 0.0091 0.0796 0.0287 0.0820 0.0120 0.0226 0.0686 0.0235 0.0164 0.0117 0.0309 0.0331 0.0101
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0462 0.2660 0.0042 0.0092 0.0657 0.0299 0.0833 0.0106 0.0241 0.0691 0.0239 0.0304 0.0174 0.0306 0.0377 0.0226
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0433 0.3096 0.0058 0.0093 0.0829 0.0343 0.0943 0.0140 0.0250 0.0658 0.0264 0.0192 0.0188 0.0253 0.0303 0.0135
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0523 0.2695 0.0038 0.0672 0.0289 0.0873 0.0158 0.0241 0.0792 0.0243 0.0275 0.0203 0.0299 0.0463 0.0229
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0463 0.3030 0.0043 0.0121 0.0827 0.0361 0.0932 0.0171 0.0249 0.0749 0.0277 0.0150 0.0173 0.0277 0.0341 0.0129
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0587 0.2801 0.0042 0.0081 0.0696 0.0240 0.0769 0.0133 0.0227 0.0621 0.0242 0.0233 0.0131 0.0269 0.0222 0.0155
WM-115 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0481 0.3345 0.0053 0.0079 0.0806 0.0347 0.0894 0.0182 0.0253 0.0636 0.0271 0.0139 0.0190 0.0258 0.0277 0.0152
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0424 0.2732 0.0040 0.0072 0.0567 0.0310 0.0683 0.0140 0.0224 0.0688 0.0237 0.0262 0.0169 0.0244 0.0454
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0479 0.2985 0.0046 0.0085 0.0754 0.0295 0.0794 0.0214 0.0250 0.0737 0.0291 0.0200 0.0145 0.0267 0.0288 0.0131
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0433 0.2674 0.0035 0.0087 0.0593 0.0271 0.0723 0.0174 0.0222 0.0577 0.0280 0.0225 0.0176 0.0223 0.0385 0.0140
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0450 0.2976 0.0074 0.0086 0.0753 0.0328 0.0817 0.0176 0.0240 0.0622 0.0323 0.0172 0.0194 0.0226 0.0275 0.0151
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0402 0.2625 0.0049 0.0153 0.0651 0.0287 0.0805 0.0154 0.0215 0.0692 0.0274 0.0255 0.0203 0.0250 0.0457 0.0135
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0434 0.3058 0.0130 0.0818 0.0328 0.0918 0.0169 0.0232 0.0741 0.0315 0.0155 0.0187 0.0260 0.0244 0.0148
WM-115 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0529 0.2755 0.0039 0.0095 0.0628 0.0233 0.0710 0.0212 0.0198 0.0589 0.0235 0.0132 0.0123 0.0238 0.0211 0.0129