Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0867 0.3226 0.0043 0.0035 0.0209 0.1873 0.1996 0.0240 0.0376 0.0927 0.0318 0.0421 0.0473 0.0093 0.0073 0.0045 0.0167 0.0497 0.0055 0.0553 0.0215
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0631 0.3077 0.0028 0.0032 0.0074 0.0683 0.0868 0.0193 0.0135 0.1044 0.0115 0.0233 0.0282 0.0059 0.0056 0.0035 0.0116 0.0161 0.0035 0.0275 0.0235
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0477 0.3521 0.0035 0.0024 0.0087 0.0637 0.0844 0.0169 0.0212 0.1188 0.0121 0.0259 0.0264 0.0061 0.0052 0.0040 0.0134 0.0179 0.0033 0.0402 0.0181
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1125 0.3906 0.0041 0.0029 0.0153 0.1572 0.1164 0.0281 0.0222 0.1401 0.0258 0.0373 0.0382 0.0084 0.0075 0.0043 0.0136 0.0303 0.0040 0.0364 0.0203
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0912 0.4546 0.0041 0.0027 0.0141 0.1263 0.1026 0.0214 0.0232 0.1488 0.0195 0.0392 0.0371 0.0068 0.0066 0.0039 0.0130 0.0177 0.0030 0.0479 0.0157
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0617 0.3268 0.0030 0.0027 0.0045 0.0621 0.0773 0.0199 0.0129 0.1309 0.0110 0.0225 0.0270 0.0044 0.0056 0.0033 0.0116 0.0116 0.0033 0.0307 0.0224
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0577 0.3861 0.0033 0.0026 0.0065 0.0693 0.0894 0.0182 0.0155 0.1393 0.0108 0.0212 0.0262 0.0044 0.0059 0.0034 0.0151 0.0116 0.0029 0.0400 0.0263
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0878 0.3472 0.0028 0.0025 0.0130 0.1416 0.0816 0.0194 0.0215 0.0867 0.0179 0.0361 0.0351 0.0078 0.0061 0.0032 0.0116 0.0281 0.0037 0.0508 0.0162
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0736 0.3068 0.0028 0.0025 0.0128 0.1174 0.0649 0.0232 0.0203 0.0977 0.0169 0.0339 0.0341 0.0055 0.0053 0.0033 0.0108 0.0249 0.0040 0.0435 0.0221
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0406 0.3115 0.0020 0.0021 0.0047 0.0589 0.0514 0.0177 0.0127 0.1041 0.0092 0.0157 0.0223 0.0035 0.0056 0.0028 0.0131 0.0091 0.0024 0.0230 0.0221
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0387 0.3460 0.0027 0.0024 0.0059 0.0565 0.0569 0.0168 0.0103 0.1167 0.0099 0.0147 0.0208 0.0037 0.0047 0.0029 0.0116 0.0114 0.0024 0.0223 0.0212
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0702 0.3436 0.0025 0.0020 0.0110 0.1011 0.0825 0.0214 0.0165 0.1185 0.0165 0.0282 0.0327 0.0077 0.0058 0.0027 0.0108 0.0207 0.0027 0.0300 0.0138
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0679 0.3745 0.0027 0.0021 0.0113 0.0894 0.0491 0.0148 0.0165 0.1319 0.0144 0.0228 0.0255 0.0050 0.0050 0.0028 0.0095 0.0152 0.0026 0.0337 0.0169
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0498 0.3155 0.0023 0.0022 0.0036 0.0506 0.0528 0.0150 0.0124 0.1339 0.0089 0.0164 0.0221 0.0035 0.0040 0.0028 0.0096 0.0084 0.0026 0.0212 0.0123
WM-115 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0509 0.3718 0.0030 0.0021 0.0051 0.0567 0.0527 0.0143 0.0118 0.1355 0.0087 0.0167 0.0214 0.0043 0.0047 0.0030 0.0104 0.0099 0.0026 0.0242 0.0138
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0840 0.2976 0.0025 0.0024 0.0152 0.1152 0.0721 0.0161 0.0198 0.1376 0.0192 0.0302 0.0302 0.0063 0.0058 0.0036 0.0115 0.0216 0.0030 0.0494 0.0152
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0786 0.3049 0.0029 0.0026 0.0120 0.1319 0.0894 0.0139 0.0179 0.1199 0.0203 0.0302 0.0345 0.0058 0.0060 0.0055 0.0143 0.0029 0.0411 0.0128
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0581 0.3125 0.0023 0.0024 0.0056 0.0515 0.0578 0.0161 0.0125 0.1226 0.0094 0.0162 0.0222 0.0042 0.0050 0.0028 0.0124 0.0126 0.0032 0.0224 0.0197
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0557 0.3436 0.0030 0.0026 0.0056 0.0608 0.0576 0.0125 0.0104 0.1312 0.0097 0.0178 0.0248 0.0046 0.0044 0.0028 0.0133 0.0126 0.0028 0.0261 0.0142
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0901 0.3345 0.0031 0.0148 0.1460 0.0911 0.0226 0.1277 0.0223 0.0377 0.0393 0.0082 0.0063 0.0054 0.0136 0.0278 0.0031 0.0527 0.0213
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1001 0.3650 0.0035 0.0025 0.0134 0.1202 0.0610 0.0201 0.0212 0.1319 0.0187 0.0341 0.0435 0.0068 0.0052 0.0035 0.0141 0.0207 0.0033 0.0627 0.0233
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0618 0.2899 0.0025 0.0023 0.0042 0.0610 0.0555 0.0160 0.0132 0.1122 0.0094 0.0156 0.0242 0.0039 0.0050 0.0027 0.0117 0.0078 0.0027 0.0336 0.0234
WM-115 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0647 0.3650 0.0030 0.0025 0.0058 0.0629 0.0610 0.0133 0.0124 0.1391 0.0095 0.0184 0.0267 0.0047 0.0047 0.0031 0.0133 0.0110 0.0026 0.0325 0.0270
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0549 0.3106 0.0026 0.0026 0.0102 0.0676 0.0732 0.0179 0.0139 0.1393 0.0128 0.0198 0.0240 0.0058 0.0053 0.0027 0.0106 0.0134 0.0032 0.0245 0.0165
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0603 0.3058 0.0026 0.0019 0.0076 0.0625 0.0537 0.0120 0.0123 0.1227 0.0097 0.0165 0.0243 0.0057 0.0046 0.0027 0.0091 0.0094 0.0030 0.0317 0.0152