Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0515 0.3774 0.0030 0.0020 0.0045 0.0235 0.0490 0.0109 0.0095 0.0995 0.0085 0.0158 0.0209 0.0036 0.0027 0.0107 0.0077 0.0023 0.0139 0.0169
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0659 0.2985 0.0022 0.0018 0.0048 0.0308 0.0513 0.0113 0.0099 0.0914 0.0113 0.0175 0.0195 0.0036 0.0073 0.0023 0.0114 0.0023 0.0149 0.0159
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0562 0.3290 0.0024 0.0015 0.0034 0.0257 0.0371 0.0108 0.0087 0.1053 0.0110 0.0131 0.0196 0.0029 0.0068 0.0020 0.0089 0.0066 0.0024 0.0146 0.0155
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0478 0.2841 0.0021 0.0017 0.0033 0.0221 0.0495 0.0115 0.0111 0.1016 0.0096 0.0122 0.0195 0.0036 0.0072 0.0024 0.0091 0.0066 0.0027 0.0124 0.0161
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0500 0.3401 0.0026 0.0016 0.0032 0.0217 0.0492 0.0100 0.0108 0.1179 0.0091 0.0116 0.0191 0.0042 0.0022 0.0092 0.0067 0.0021 0.0137 0.0197
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0524 0.2967 0.0025 0.0023 0.0042 0.0252 0.0473 0.0121 0.0116 0.1006 0.0114 0.0157 0.0245 0.0055 0.0076 0.0031 0.0100 0.0112 0.0028 0.0170 0.0221
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0482 0.3472 0.0026 0.0017 0.0037 0.0257 0.0377 0.0108 0.0089 0.1046 0.0115 0.0166 0.0243 0.0050 0.0074 0.0029 0.0097 0.0110 0.0021 0.0162 0.0172
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0516 0.2933 0.0026 0.0022 0.0028 0.0215 0.0433 0.0118 0.0094 0.0992 0.0090 0.0188 0.0217 0.0033 0.0072 0.0024 0.0101 0.0060 0.0022 0.0201 0.0212
WM-115 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0571 0.3571 0.0029 0.0020 0.0041 0.0227 0.0493 0.0109 0.0091 0.1131 0.0093 0.0150 0.0230 0.0035 0.0027 0.0109 0.0076 0.0022 0.0174
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0621 0.2833 0.0025 0.0022 0.0042 0.0265 0.0711 0.0164 0.0101 0.0568 0.0100 0.0168 0.0224 0.0039 0.0091 0.0025 0.0123 0.0074 0.0025 0.0149 0.0165
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0653 0.3096 0.0030 0.0021 0.0042 0.0213 0.0586 0.0116 0.0087 0.0633 0.0091 0.0126 0.0216 0.0035 0.0093 0.0025 0.0111 0.0048 0.0026 0.0167 0.0188
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0628 0.2545 0.0031 0.0023 0.0035 0.0205 0.0773 0.0155 0.0142 0.0610 0.0083 0.0099 0.0187 0.0037 0.0084 0.0027 0.0112 0.0055 0.0031 0.0145
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0538 0.2890 0.0023 0.0023 0.0041 0.0240 0.0680 0.0129 0.0102 0.0841 0.0082 0.0105 0.0259 0.0039 0.0058 0.0029 0.0120 0.0065 0.0025 0.0208 0.0131
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0649 0.3155 0.0030 0.0020 0.0036 0.0214 0.0444 0.0085 0.0094 0.0918 0.0079 0.0106 0.0222 0.0037 0.0062 0.0028 0.0086 0.0067 0.0030 0.0212 0.0133
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
WM-115 0.0000
uM
 10
hr
 8
InnoScan/Mapix
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0078 0.3610 0.0025 0.0023 0.0051 0.0499 0.0757 0.0122 0.0069 0.1028 0.0135 0.0158 0.0601 0.0052 0.0066 0.0033 0.0085 0.0168 0.0055 0.0071 0.0403
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0086 0.3356 0.0031 0.0026 0.0062 0.0416 0.0732 0.0093 0.0070 0.1026 0.0113 0.0127 0.0668 0.0057 0.0053 0.0033 0.0115 0.0149 0.0048 0.0083 0.0370
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0082 0.3096 0.0023 0.0021 0.0040 0.0399 0.0770 0.0098 0.0086 0.0850 0.0102 0.0160 0.0650 0.0053 0.0051 0.0030 0.0103 0.0148 0.0050 0.0093 0.0327
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0078 0.3436 0.0027 0.0026 0.0063 0.0411 0.0769 0.0101 0.0076 0.0914 0.0109 0.0116 0.0710 0.0052 0.0050 0.0036 0.0119 0.0169 0.0045 0.0120 0.0474
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0985 0.3509 0.0036 0.0032 0.0083 0.0808 0.0822 0.0218 0.0193 0.1488 0.0129 0.0272 0.0332 0.0063 0.0066 0.0041 0.0104 0.0145 0.0036 0.0317 0.0202
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0763 0.4149 0.0042 0.0031 0.0073 0.0836 0.0891 0.0198 0.0186 0.1508 0.0131 0.0276 0.0351 0.0057 0.0068 0.0043 0.0112 0.0113 0.0031 0.0386 0.0171
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0698 0.3175 0.0033 0.0029 0.0040 0.0612 0.0817 0.0234 0.0155 0.1230 0.0089 0.0213 0.0302 0.0044 0.0068 0.0033 0.0160 0.0082 0.0034 0.0279 0.0329
WM-115 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0735 0.3953 0.0043 0.0036 0.0059 0.0725 0.0991 0.0224 0.0159 0.1290 0.0092 0.0166 0.0320 0.0044 0.0066 0.0038 0.0163 0.0109 0.0031 0.0318 0.0236