Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0654 0.3165 0.0034 0.0018 0.0119 0.0513 0.0657 0.0096 0.0120 0.0729 0.0131 0.0183 0.0402 0.0093 0.0074 0.0025 0.0084 0.0172 0.0035 0.0140 0.0138
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0458 0.2959 0.0024 0.0018 0.0076 0.0449 0.0711 0.0107 0.0113 0.0589 0.0112 0.0153 0.0391 0.0052 0.0056 0.0031 0.0101 0.0137 0.0029 0.0141 0.0089
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0527 0.3068 0.0023 0.0019 0.0067 0.0397 0.0534 0.0075 0.0091 0.0883 0.0097 0.0134 0.0373 0.0057 0.0054 0.0026 0.0084 0.0123 0.0028 0.0126 0.0099
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0695 0.3030 0.0025 0.0020 0.0112 0.0671 0.0678 0.0119 0.0120 0.0670 0.0159 0.0193 0.0532 0.0080 0.0054 0.0036 0.0114 0.0187 0.0032 0.0158 0.0166
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0680 0.3448 0.0023 0.0021 0.0103 0.0577 0.0510 0.0096 0.0110 0.0845 0.0143 0.0176 0.0451 0.0080 0.0061 0.0026 0.0081 0.0167 0.0030 0.0154 0.0114
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0481 0.3356 0.0028 0.0017 0.0071 0.0423 0.0781 0.0093 0.0092 0.0742 0.0102 0.0131 0.0333 0.0056 0.0064 0.0026 0.0091 0.0110 0.0031 0.0134 0.0155
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0497 0.3484 0.0029 0.0022 0.0072 0.0450 0.0663 0.0085 0.0097 0.0991 0.0089 0.0143 0.0315 0.0054 0.0055 0.0024 0.0096 0.0127 0.0026 0.0129 0.0178
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0841 0.2857 0.0031 0.0023 0.0094 0.0887 0.0775 0.0135 0.0707 0.0219 0.0306 0.0664 0.0090 0.0076 0.0024 0.0087 0.0285 0.0030 0.0193 0.0176
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0772 0.3534 0.0023 0.0015 0.0109 0.0744 0.0661 0.0127 0.0159 0.0709 0.0185 0.0323 0.0595 0.0063 0.0072 0.0021 0.0100 0.0229 0.0194 0.0108
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0534 0.2770 0.0024 0.0022 0.0047 0.0417 0.0443 0.0113 0.0080 0.0847 0.0104 0.0153 0.0326 0.0046 0.0052 0.0026 0.0067 0.0112 0.0025 0.0104 0.0116
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0462 0.3436 0.0024 0.0022 0.0057 0.0432 0.0618 0.0106 0.0089 0.0847 0.0108 0.0143 0.0333 0.0042 0.0061 0.0031 0.0082 0.0109 0.0032 0.0127 0.0072
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0966 0.3205 0.0026 0.0023 0.0136 0.0821 0.0649 0.0153 0.0131 0.0835 0.0174 0.0294 0.0582 0.0101 0.0083 0.0023 0.0080 0.0245 0.0028 0.0138 0.0139
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0767 0.3663 0.0029 0.0022 0.0125 0.0753 0.0607 0.0148 0.0140 0.0906 0.0165 0.0280 0.0571 0.0068 0.0075 0.0028 0.0093 0.0155 0.0061 0.0176 0.0086
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0456 0.3077 0.0026 0.0017 0.0041 0.0445 0.0532 0.0109 0.0086 0.0876 0.0090 0.0167 0.0343 0.0035 0.0062 0.0025 0.0071 0.0095 0.0025 0.0102 0.0124
WM-115 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0470 0.3484 0.0028 0.0021 0.0057 0.0502 0.0633 0.0097 0.0092 0.0881 0.0089 0.0157 0.0355 0.0044 0.0057 0.0026 0.0085 0.0111 0.0025 0.0129 0.0098
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0861 0.2725 0.0024 0.0018 0.0115 0.0855 0.0125 0.0182 0.0750 0.0129 0.0345 0.0563 0.0092 0.0094 0.0024 0.0103 0.0028 0.0206 0.0127
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0804 0.3155 0.0029 0.0019 0.0094 0.0758 0.0529 0.0108 0.0168 0.0820 0.0129 0.0315 0.0584 0.0066 0.0078 0.0023 0.0097 0.0172 0.0216 0.0104
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0431 0.2660 0.0017 0.0020 0.0049 0.0428 0.0438 0.0108 0.0090 0.0833 0.0071 0.0148 0.0298 0.0043 0.0081 0.0024 0.0069 0.0102 0.0022 0.0086 0.0114
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0428 0.3096 0.0026 0.0018 0.0055 0.0414 0.0444 0.0101 0.0083 0.0943 0.0072 0.0140 0.0311 0.0041 0.0067 0.0023 0.0076 0.0107 0.0022 0.0097 0.0085
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0555 0.2695 0.0017 0.0021 0.0095 0.0554 0.0490 0.0105 0.0106 0.0873 0.0135 0.0188 0.0415 0.0062 0.0056 0.0069 0.0144 0.0021 0.0100 0.0099
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0558 0.3030 0.0026 0.0014 0.0091 0.0547 0.0401 0.0087 0.0097 0.0932 0.0141 0.0164 0.0394 0.0049 0.0054 0.0019 0.0075 0.0116 0.0020 0.0113 0.0084
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0440 0.2801 0.0019 0.0020 0.0030 0.0353 0.0539 0.0084 0.0082 0.0769 0.0074 0.0124 0.0278 0.0035 0.0053 0.0021 0.0074 0.0058 0.0026 0.0085 0.0144
WM-115 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0426 0.3345 0.0027 0.0015 0.0047 0.0388 0.0556 0.0086 0.0084 0.0894 0.0080 0.0115 0.0289 0.0041 0.0050 0.0023 0.0105 0.0069 0.0023 0.0099 0.0130
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0554 0.2732 0.0023 0.0021 0.0072 0.0489 0.0553 0.0683 0.0111 0.0182 0.0373 0.0064 0.0059 0.0033 0.0068 0.0156 0.0022 0.0148 0.0129
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0558 0.2985 0.0025 0.0015 0.0059 0.0431 0.0605 0.0075 0.0095 0.0794 0.0100 0.0162 0.0360 0.0047 0.0068 0.0023 0.0084 0.0120 0.0022 0.0147 0.0084