Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5650 1.0834 0.2114 0.3096 0.2457 0.6944 0.4831 0.3731 0.7463 0.2695 0.6061 0.2618 0.3597 0.2179 0.3195 0.2188 0.8265 0.3356
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5587 1.1891 0.2066 0.3175 0.2268 0.6803 0.4405 0.3279 0.6250 0.2506 0.4854 0.2625 0.2445 0.4525 0.2020 0.3077 0.2151 0.6849 0.3106
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5618 1.0989 0.1934 0.3311 0.2469 0.6897 0.4651 0.2950 0.6061 0.2331 0.5405 0.2725 0.2252 0.4386 0.2169 0.2755 0.2037 0.6993 0.3077
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5348 1.0438 0.1727 0.2924 0.2193 0.6711 0.4505 0.4274 0.6667 0.2433 0.4808 0.2584 0.2160 0.4167 0.2110 0.2874 0.1859 0.6711 0.3115
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5405 1.0638 0.2049 0.2639 0.2212 0.6667 0.4831 0.3268 0.6623 0.2353 0.5682 0.2591 0.2381 0.4587 0.2066 0.3125 0.2123 0.6803 0.3195
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5208 1.1682 0.2237 0.3058 0.2336 0.6623 0.4717 0.2924 0.6623 0.2506 0.4975 0.2762 0.2545 0.3546 0.1972 0.3610 0.2049 0.6993 0.3145
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5263 1.1025 0.2268 0.3356 0.2584 0.6329 0.3546 0.6667 0.2315 0.5952 0.2747 0.2488 0.3460 0.2000 0.3322 0.2179 0.7143 0.3106
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5319 1.0246 0.1828 0.3077 0.2577 0.6369 0.4132 0.3145 0.6897 0.2193 0.4566 0.2899 0.2198 0.3247 0.2179 0.3058 0.1835 0.6494
RVH-421 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.5465 1.0560 0.2041 0.2801 0.2681 0.6452 0.4367 0.3731 0.6757 0.2404 0.5435 0.2916 0.2564 0.3610 0.2273 0.3279 0.2247 0.7463 0.3040
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5556 1.0363 0.2169 0.3268 0.2179 0.6849 0.4184 0.2916 0.6024 0.2326 0.4902 0.2558 0.2326 0.4000 0.2174 0.2941 0.1938 0.6849 0.3185
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5465 0.9901 0.2053 0.2916 0.2320 0.6803 0.4854 0.3378 0.5682 0.2155 0.5405 0.2611 0.2217 0.3861 0.2198 0.2597 0.1866 0.6993 0.3279
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5587 1.0121 0.1866 0.3247 0.2079 0.6623 0.4505 0.2985 0.6579 0.2353 0.5076 0.2710 0.2475 0.3906 0.2212 0.2667 0.1862 0.6623 0.3413
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5405 0.9804 0.1883 0.2959 0.2326 0.6579 0.4329 0.3636 0.6711 0.2257 0.5435 0.2770 0.2451 0.4149 0.2237 0.2849 0.1919 0.7194 0.3356
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5291 1.1416 0.2141 0.3165 0.2387 0.6623 0.4587 0.3236 0.6711 0.5128 0.3012 0.2370 0.3345 0.2066 0.3345 0.2053 0.6993 0.3367
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5236 1.0989 0.1942 0.2941 0.2433 0.6711 0.4587 0.3704 0.6667 0.2439 0.5747 0.2899 0.2336 0.3367 0.2155 0.3012 0.2174 0.7407 0.3096
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5618 1.0582 0.2020 0.3436 0.2165 0.6711 0.4348 0.3472 0.7407 0.2288 0.5181 0.2825 0.2364 0.3484 0.2381 0.2695 0.1942 0.6944 0.3333
RVH-421 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.5714 1.0395 0.2208 0.3534 0.2294 0.6944 0.4367 0.4115 0.7692 0.2375 0.5650 0.3021 0.3663 0.2278 0.3247 0.2169 0.7634 0.3367
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5405 0.9434 0.1730 0.3012 0.1980 0.5714 0.3788 0.2950 0.7813 0.2132 0.4367 0.2494 0.2075 0.4274 0.2146 0.2994 0.1748 0.5952 0.3333
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5263 0.8929 0.1721 0.2899 0.2105 0.5587 0.4167 0.2959 0.6757 0.2160 0.4608 0.2203 0.2165 0.4348 0.2179 0.2725 0.1767 0.6061 0.3215
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5882 0.9091 0.1957 0.3448 0.1949 0.5952 0.4032 0.3135 0.6494 0.2110 0.4132 0.2618 0.2273 0.4386 0.2364 0.2513 0.1770 0.6410 0.3425
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.6024 1.0638 0.1842 0.3049 0.2062 0.6061 0.4255 0.3247 0.7143 0.2439 0.4386 0.3012 0.2564 0.4587 0.2688 0.2364 0.1862 0.6944 0.3534
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5952 0.9709 0.2105 0.3636 0.2155 0.6369 0.4167 0.3745 0.8130 0.2404 0.5102 0.2933 0.2392 0.3817 0.2439 0.2695 0.1835 0.6849 0.3846
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5848 1.0194 0.2037 0.3559 0.2299 0.6289 0.4425 0.3745 0.7407 0.2558 0.5405 0.3279 0.2532 0.3623 0.2347 0.2703 0.2037 0.7143 0.3521
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5618 0.8929 0.1890 0.3521 0.2179 0.6173 0.4310 0.3460 0.7194 0.2123 0.4310 0.2890 0.2320 0.3367 0.2433 0.2506 0.1887 0.7194 0.3290
RVH-421 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.5917 1.0549 0.1996 0.3268 0.2268 0.6173 0.4608 0.3636 0.7519 0.2433 0.4546 0.3257 0.2564 0.3472 0.2304 0.2793 0.2105 0.7299 0.3333