Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5051 0.1873 0.3236 0.2024 0.6410 0.4292 0.3003 0.6944 0.4739 0.2786 0.2299 0.3205 0.2066 0.2571 0.2096 0.6623 0.2959
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5405 1.0373 0.2169 0.3390 0.2433 0.6452 0.4566 0.3731 0.7634 0.2451 0.5348 0.2625 0.3584 0.2101 0.3311 0.2028 0.8065 0.3236
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5376 1.1494 0.2096 0.2950 0.2212 0.7246 0.4608 0.3086 0.6757 0.2331 0.5181 0.2681 0.2232 0.3436 0.2146 0.2933 0.2092 0.6803 0.3311
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5208 1.1074 0.2058 0.2674 0.2597 0.7042 0.4739 0.3636 0.7246 0.2463 0.5714 0.2433 0.2416 0.3390 0.1876 0.2924 0.1880 0.7092 0.3068
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5291 1.1751 0.1972 0.2833 0.2105 0.6944 0.4546 0.3165 0.8000 0.2375 0.5587 0.2646 0.2375 0.3690 0.1953 0.3096 0.2198 0.6711 0.3367
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5263 1.1161 0.2169 0.2801 0.2513 0.6803 0.4484 0.3984 0.7752 0.2571 0.6211 0.2703 0.2611 0.4000 0.1969 0.3135 0.2174 0.6993 0.3185
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5319 1.2005 0.2096 0.3040 0.2222 0.6757 0.4444 0.3356 0.7353 0.2359 0.5587 0.2985 0.2222 0.3484 0.2008 0.3390 0.2268 0.6897 0.3378
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5051 1.1299 0.2252 0.2959 0.2179 0.6711 0.4405 0.3731 0.7143 0.2342 0.6329 0.2841 0.2611 0.3597 0.2083 0.3311 0.2174 0.6993 0.3279
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5435 1.0604 0.2062 0.3378 0.2101 0.6623 0.4525 0.3300 0.7519 0.2262 0.5128 0.2660 0.2155 0.3704 0.2208 0.2762 0.1919 0.7143 0.3571
RVH-421 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.5556 1.0406 0.2315 0.3322 0.2392 0.6711 0.4525 0.4310 0.8065 0.2545 0.5682 0.2976 0.2695 0.3731 0.2304 0.3571 0.2288 0.3378
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5465 1.3263 0.2041 0.2817 0.7299 0.4785 0.3300 0.8475 0.2364 0.5917 0.3058 0.2342 0.3690 0.1972 0.2041 0.6803 0.3322
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 1.1696 0.1862 0.2967 0.2457 0.6944 0.4608 0.3185 0.7246 0.2469 0.5988 0.2849 0.2469 0.3861 0.1934 0.3096 0.2404 0.6667 0.3322
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5495 0.9804 0.2096 0.3077 0.2165 0.6757 0.4546 0.3279 0.7407 0.2278 0.5405 0.2632 0.2217 0.3759 0.2237 0.2778 0.1773 0.6667 0.3378
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5556 1.2300 0.2075 0.2985 0.2416 0.6536 0.4651 0.3460 0.7299 0.2370 0.5917 0.3195 0.2618 0.3704 0.2227 0.2967 0.2232 0.6944 0.3300
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5650 1.0834 0.1980 0.2916 0.2331 0.6623 0.4348 0.2985 0.7246 0.2404 0.5587 0.2710 0.2353 0.1949 0.2959 0.2028 0.6369
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5525 1.2853 0.2141 0.2801 0.2315 0.5988 0.4546 0.3215 0.6757 0.2584 0.5848 0.3356 0.2451 0.3597 0.2012 0.2985 0.2203 0.6849 0.3096
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.5405 0.9709 0.1946 0.3175 0.2008 0.6024 0.4184 0.3125 0.7813 0.2326 0.4785 0.2591 0.2310 0.2141 0.2674 0.1992 0.6623 0.3195
RVH-421 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 1.1834 0.2174 0.2967 0.2336 0.6098 0.4566 0.3425 0.8621 0.2506 0.5319 0.3115 0.2558 0.3718 0.2088 0.2786 0.2618 0.6897 0.3257
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5263 1.2077 0.2000 0.3185 0.2398 0.7407 0.4975 0.3226 0.6849 0.2865 0.5236 0.2681 0.2532 0.3984 0.2066 0.3096 0.2096 0.7463 0.3300
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5465 1.2048 0.3436 0.3058 0.7246 0.4808 0.3623 0.6667 0.3012 0.5495 0.3125 0.2591 0.4082 0.2169 0.3003 0.2500 0.7143 0.3534
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5495 1.0965 0.2012 0.3677 0.2326 0.7042 0.4951 0.3268 0.7519 0.2571 0.5780 0.2817 0.2551 0.3984 0.2353 0.2882 0.1961 0.7092 0.3135
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5682 1.1507 0.2132 0.3460 0.2513 0.6849 0.4951 0.3650 0.8130 0.2740 0.5848 0.3195 0.2577 0.4032 0.2294 0.3021 0.2169 0.7692 0.3195
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5714 1.1481 0.1984 0.3610 0.2604 0.7299 0.4739 0.3135 0.6849 0.2564 0.5376 0.2907 0.2591 0.3534 0.2299 0.3571 0.2288 0.7143 0.3311
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5435 1.1820 0.2203 0.3636 0.2959 0.7143 0.3497 0.6757 0.2667 0.5814 0.2907 0.3571 0.2336 0.3077 0.2500 0.6849 0.3401
RVH-421 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.5291 1.0846 0.2092 0.3559 0.2212 0.6944 0.4587 0.3460 0.7519 0.2571 0.5435 0.2933 0.2463 0.3448 0.2375 0.3333 0.2092 0.7519 0.3012