Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.1761 0.8000 0.0059 0.0045 0.0057 0.0097 0.0085 0.2066 0.0071 0.3484 0.0166 0.0109 0.0052 0.0101 0.0053 0.0176 0.0073 0.0043 0.0941 0.0882
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.1927 0.6944 0.0056 0.0044 0.0068 0.0104 0.0098 0.1504 0.0097 0.4878 0.0157 0.0094 0.0052 0.0097 0.0042 0.0128 0.0077 0.0058 0.0804 0.0448
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.2075 0.7813 0.0073 0.0060 0.0071 0.0137 0.1610 0.0092 0.3690 0.0179 0.0107 0.0110 0.0056 0.0147 0.0097 0.0054 0.0818 0.0622
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.2012 0.4717 0.0039 0.0041 0.0065 0.0081 0.0095 0.2500 0.0092 0.2740 0.0167 0.0091 0.0080 0.0073 0.0198 0.0069 0.0067 0.0728 0.0409
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.2237 0.5587 0.0056 0.0059 0.0053 0.0106 0.0094 0.2227 0.0069 0.3021 0.0090 0.0052 0.0090 0.0073 0.0249 0.0088 0.0050 0.0780 0.0455
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1087 0.5682 0.0104 0.0083 0.0103 0.0110 0.0170 0.1316 0.0180 0.1773 0.0134 0.0094 0.0042 0.0083 0.0034 0.0160 0.0113 0.0060 0.0624 0.0293
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1282 0.5952 0.0117 0.0094 0.0089 0.0143 0.0178 0.1425 0.0167 0.1992 0.0140 0.0044 0.0080 0.0047 0.0161 0.0109 0.0037 0.0657 0.0284
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1502 0.6536 0.0050 0.0052 0.0065 0.0085 0.0098 0.1639 0.0108 0.2770 0.0175 0.0093 0.0042 0.0089 0.0042 0.0141 0.0071 0.0059 0.0687 0.1135
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1704 0.6667 0.0076 0.0072 0.0067 0.0110 0.0114 0.1595 0.0106 0.2725 0.0197 0.0097 0.0053 0.0066 0.0151 0.0087 0.0052 0.0692 0.1307
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1220 0.5465 0.0052 0.0049 0.0057 0.0077 0.0096 0.1482 0.0086 0.1287 0.0124 0.0085 0.0041 0.0080 0.0031 0.0109 0.0062 0.0047 0.0482 0.0429
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1236 0.5682 0.0060 0.0056 0.0053 0.0101 0.0094 0.1374 0.0088 0.1399 0.0134 0.0087 0.0049 0.0083 0.0042 0.0090 0.0065 0.0037 0.0463 0.0474
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.1894 0.5291 0.0059 0.0037 0.0056 0.0088 0.0083 0.0070 0.2079 0.0177 0.0079 0.0039 0.0082 0.0048 0.0139 0.0055 0.0037 0.0536 0.0546
RVH-421 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.2299 0.5882 0.0073 0.0064 0.0063 0.0117 0.0112 0.1876 0.0096 0.2208 0.0192 0.0094 0.0048 0.0085 0.0065 0.0156 0.0091 0.0063 0.0533 0.0487
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.2049 0.4405 0.0109 0.0130 0.0105 0.0131 0.0206 0.1482 0.0232 0.2924 0.0280 0.0069 0.0038 0.0061 0.0061 0.0399 0.0272 0.1248 0.0617
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.2174 0.4608 0.0150 0.0125 0.0078 0.0169 0.0252 0.1471 0.0265 0.3378 0.0283 0.0074 0.0043 0.0061 0.0091 0.0440 0.0183 0.0069 0.1220 0.0622
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.1355 0.6289 0.0111 0.0100 0.0097 0.0130 0.0188 0.1339 0.0231 0.2053 0.0170 0.0081 0.0041 0.0084 0.0036 0.0129 0.0235 0.0049 0.0529 0.0647
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.1353 0.6410 0.0173 0.0150 0.0094 0.0167 0.0236 0.1299 0.0271 0.2053 0.0196 0.0095 0.0054 0.0093 0.0064 0.0122 0.0148 0.0041 0.0575 0.0540
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.1094 0.5348 0.0063 0.0059 0.0068 0.0087 0.0095 0.1629 0.0113 0.1445 0.0134 0.0090 0.0038 0.0072 0.0031 0.0143 0.0111 0.0050 0.0575 0.0327
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.1242 0.5714 0.0072 0.0062 0.0060 0.0106 0.0109 0.1482 0.0134 0.1592 0.0129 0.0092 0.0051 0.0076 0.0086 0.0149 0.0086 0.0033 0.0620 0.0491
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
RVH-421 0.0000
uM
 5
hr
 3
InnoScan/Mapix
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.2208 0.4566 0.0137 0.0124 0.0159 0.0318 0.1439 0.0276 0.3185 0.0341 0.0071 0.0041 0.0062 0.0075 0.0351 0.0288 0.0098 0.0923 0.0502
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.2445 0.4444 0.0161 0.0108 0.0086 0.0183 0.0283 0.1357 0.0329 0.3236 0.0329 0.0073 0.0044 0.0062 0.0096 0.0396 0.0327 0.0026 0.1138 0.1025