Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6494 1.1507 0.1381 0.1642 0.1473 0.2604 0.1707 0.2681 0.9615 0.3953 0.2778 0.1908 0.2262 0.1764 0.3534 0.0529 0.0935 0.4325
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6711 1.1136 0.1159 0.1414 0.1323 0.2809 0.1859 0.2950 0.8850 0.3401 0.2967 0.1873 0.2310 0.3953 0.1046 0.0781 0.5347
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.7194 2.0284 0.1211 0.2288 0.1412 0.4405 0.1672 0.2833 0.8403 0.4032 0.3597 0.1550 0.1658 0.1484 0.3650 0.0877 0.0990 0.5618 0.5678
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5618 1.3947 0.1372 0.2114 0.1522 0.3636 0.1701 0.3205 0.6579 0.3175 0.3817 0.1458 0.1704 0.1560 0.3497 0.0794 0.0709 0.6579 0.3978
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5848 1.1962 0.1460 0.2088 0.1965 0.3115 0.1923 0.3425 0.7092 0.3021 0.5076 0.1873 0.1869 0.1799 0.3968 0.0806 0.0901 0.6250 0.4230
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.6024 1.1249 0.1484 0.2237 0.2119 0.3049 0.2174 0.3937 0.6098 0.3086 0.4808 0.1876 0.1934 0.1802 0.4367 0.1326 0.0719 0.6452 0.4170
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.7634 2.0367 0.1226 0.1828 0.1258 0.4464 0.1479 0.2262 0.8197 0.4082 0.3484 0.1407 0.1718 0.1431 0.3401 0.0725 0.1142 0.5155 0.5696
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5952 1.4006 0.1156 0.1832 0.1421 0.3401 0.1686 0.2632 0.6536 0.3125 0.3195 0.1414 0.1764 0.1543 0.3401 0.0769 0.0794 0.5814 0.4122
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5952 1.1136 0.1541 0.2364 0.2288 0.3040 0.2387 0.3436 0.6452 0.3012 0.3378 0.2137 0.2359 0.1912 0.4016 0.1715 0.0885 0.6849 0.4211
COLO 858 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.6061 1.0823 0.1585 0.2457 0.2304 0.2941 0.2132 0.3571 0.6452 0.3077 0.3636 0.2119 0.2439 0.1876 0.4219 0.1799 0.0971 0.6849 0.3901
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.9174 2.3310 0.1357 0.2398 0.1355 0.4951 0.1407 0.2551 0.8929 0.4484 0.4348 0.1445 0.1779 0.1692 0.3236 0.0532 0.1103 0.5525 0.6148
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6135 1.2821 0.1316 0.2208 0.1284 0.3378 0.1656 0.2688 0.5848 0.2882 0.4367 0.1381 0.1916 0.1923 0.3367 0.0990 0.0741 0.6757 0.4124
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6173 1.3123 0.1236 0.1949 0.1661 0.3345 0.1718 0.2849 0.6211 0.2950 0.3460 0.1473 0.1730 0.1531 0.3802 0.0559 0.0758 0.6289 0.4041
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6024 1.0707 0.1416 0.2008 0.1623 0.2933 0.1712 0.3155 0.6289 0.2639 0.3311 0.1637 0.1808 0.1701 0.3846 0.0870 0.0730 0.7042 0.3645
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.8929 2.3256 0.1111 0.1802 0.1429 0.4608 0.1473 0.2304 1.0000 0.4219 0.3058 0.1407 0.1653 0.1412 0.3345 0.0412 0.1174 0.5208 0.5680
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5682 1.2771 0.1131 0.1855 0.1534 0.3268 0.1506 0.2632 0.7042 0.2513 0.3068 0.1425 0.1721 0.1495 0.3401 0.0704 0.0645 0.6250 0.4037
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6211 1.3228 0.1366 0.2058 0.1786 0.3247 0.1919 0.3040 0.6944 0.2941 0.4032 0.1585 0.1709 0.1613 0.3861 0.0395 0.0730 0.6452 0.4282
COLO 858 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5556 1.1086 0.1412 0.2141 0.1587 0.2667 0.1792 0.3236 0.7874 0.2717 0.3584 0.1751 0.1815 0.1597 0.3831 0.0840 0.0775 0.6849 0.3657
COLO 858 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8850 2.6525 0.1733 0.2762 0.1159 0.4975 0.1838 0.3311 1.2077 0.4132 0.5000 0.1439 0.2632 0.2049 0.3745 0.0847 0.1339 0.6173 0.6404
COLO 858 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.6897 1.7065 0.1421 0.2762 0.1405 0.3984 0.1730 0.3534 0.9174 0.3058 0.5181 0.1678 0.2475 0.2208 0.4082 0.1178 0.0769 0.4866
COLO 858 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8130 2.1786 0.1115 0.1795 0.1047 0.4348 0.1439 0.2611 0.9615 0.3984 0.3650 0.1328 0.1859 0.1425 0.3003 0.0495 0.1511 0.5051 0.5350
COLO 858 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5780 1.0672 0.1351 0.1996 0.1208 0.2950 0.1515 0.2786 0.6579 0.2660 0.3546 0.1391 0.1901 0.1642 0.3425 0.0870 0.0769 0.6173 0.3502
COLO 858 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8547 2.6178 0.1092 0.1852 0.0926 0.4695 0.1284 0.2310 1.2610 0.4274 0.3135 0.1383 0.1629 0.1435 0.2985 0.0699 0.1235 0.5405 0.6368